RESUMO
OBJECTIVE: To explore the effect of phase transition process of compound L-carnitine plastics on the characters of transdermal absorption, provide a theoretical evidence for the penetration enhancing effect produced from phase transition process. METHODS: The accumulated transdermal percentage of capsaicin and L-carnitine were used as measurement indicators, the experiment was finished by improved Franz diffuser and disembodied rabbit skin, the content of different components in transdermal receiving solution and in skin that received in different time was determined by HPLC, the curve of transdermal was drawn been drawn, the rule and the characters of transdermal were analyzed. RESULTS: The transdermal dynamics curve before and after the phase transition of compound L-carnitine plastics is a kind of continuous function, while it become a kind of non-continuous function during the process of the phase transition. The 95% confidence interval of the parameters of transdermal velocity and amount between sample A with transdermal enhancer and sample B without transdermal enhancer shows overlap, the difference shows no statistical significance between sample A and B. It was showed that the sample A have the same transdermal rate and amount as B. While 95% confidence interval of the parameters of transdermal velocity and amount among sample A,B and C (without film-former materials) shows no overlap, the difference shows statistical significance among sample A,B and C. When the capsaicin was used as indicators, the transdermal rate of sample A and B respectively are 1.459 times and 1.450 times of C, the transdermal amount of sample A and B respectively are 2.53 times and 1.907 times of C. When the capsaicin was used as indicators, the transdermal rate of sample A and B respectively are 1.153 times and 1.169 times of C, the transdermal amount of sample A and B respectively are 1.722 times and 1.692 times of C. CONCLUSION: Phase transition process can significantly increase both transdermal rate and transdermal amount of compound L-carnitine plastics, the time increased by phase transition process correspond to film-former time of plastics, the penetration enhancing effect produced from phase transition process is far greater than the penetration enhancing effect produced from transdermal absorption penetration enhancers(P0.05).
RESUMO
OBJECTIVE: To provide a theoretical basis for industrialized production of compound L-carnitine coating agent by optimizing the prescription. METHODS: Using the time of coating formation as the evaluation index, Central composite design was used to optimize the ratio of film-former and plasticizer on the basis of single factor experiments. RESULTS: The film-former is 15.4% acrylic resin II, and the plasticizer is 10.4% glycerin. CONCLUSION: In this paper, the optimal formulation and dosage of compound L-carnitine coating agent was screened. This formulation can provide reference for the molding process of the preparation and the experiment of percutaneous absorption.