Avaliação de indicadores higienicossanitários em mortadelas fatiadas / Evaluation of sanitary hygienic indicators in the sliced bolognas

Os embutidos cárneos estão entre os produtos mais consumidos e comercializados e podem representar uma importante fonte de contaminação por micro-organismos patogênicos ou deteriorantes decorrentes de manipulação excessiva, do aumento da superfície de contato e pela maior exposição ao oxigênio atmosférico. Este trabalho avaliou a qualidade microbiológica de mortadelas fatiadas comercializadas em Fortaleza, CE. Foram coletadas 12 amostras de mortadelas, de diferentes marcas, em supermercados de Fortaleza, CE e estas foram fatiadas e embaladas no momento da compra. As amostras foram submetidas à avaliação microbiológica quanto à presença de coliformes termotolerantes, estafilococos coagulase positiva e presença/ausência de Salmonella spp. Os resultados obtidos demonstraram que todas as amostras analisadas estavam dentro dos padrões estabelecidos pela legislação brasileira. A presença de coliformes e estafilococos coagulase positiva nos alimentos, mesmo que seja em níveis inferiores aos preconizados pela legislação vigente, pode indicar condições higienicossanitárias insatisfatórias. Neste contexto, enfatiza-se a necessidade de efetuar a adoção de boas práticas em supermercados e maior fiscalização por parte dos órgãos responsáveis, com o intuito de minimizar os possíveis problemas de saúde pública.

Meat sausages are among the mostly consumed and commercialized products, and they are the important source of contamination by pathogenic or deteriorating microorganisms due to the excessive manipulation, the increased surface contact and the considerable exposure to the atmospheric contents. The objective of this study was to evaluate the microbiological quality of sliced mortadella commercialized in Fortaleza, CE. Twelve samples of mortadella from different brands were collected in supermarkets located in Fortaleza, CE, and they were sliced and packed at the time of purchase. The samples were evaluated by the microbiological analyses for detecting the occurrence of thermotolerant coliforms, coagulase positive staphylococci and presence/absence of Salmonella spp. The results from the present study showed that all of the analyzed samples were within the standards established by the Brazilian legislation. The occurrence of coliforms and coagulase-positive staphylococci in food, even at lower levels than those recommended by current legislation, they indicate the unsatisfactory sanitary hygienic conditions. Therefore, it is emphasized the need to adopt the good practices in supermarkets and a major supervision by the responsible agencies, in order to minimize the occurrence of possible public health problems.

Prevalência, epidemiologia, caracterização sorológica e molecular de Listeria monocytogenes isoladas na criação intensiva de novilhos superprecoces e em abatedouros frigoríficos no Estado de São Paulo / Prevalence, epidemiology, serology and molecular characterization of Listeria monocytogenes isolates in intensive creation of steers in São Paulo State abbatoirs

O Brasil é um dos principais exportadores de carne bovina. A maioria do gado é criada a pasto, porém uma parte já é confinada, como na criação de Novilhos Superprecoces. Este confinamento pode favorecer a contaminação por L. monocytogenes (Lm) do rebanho, bactéria responsável pela listeriose, doencia que provoca aborto, neuropatias e gastrenterites. Com isto, pesquisou-se a presença e característica sorotípicas e moleculares de Lm durante o confinamento de cinco raças de novilhos e em suas carcaças no frigorífico A, assim como amostras de carcaças, utensílios, equipamentos e ambiente em outro frigorífico(B). Foram utilizadas para tipar as cepas técnicas de sorotipagem por multiplex PCR, soroaglutinação PFGE...

Brazil is one of the most important beef producer/exporter country worldwide. The majority of the cattle is raised extensively, but some of them are feedloted. Confinement conditions can stress the animals and favor the contamination and proliferation for Listeria monocytogenes (Lm), agent of listeriosis which causes abortion, stillbirths, nervous dysfunctions and gastroenteritis. The aim of this study was to verify the presence of this microorganism and its molecular and serotype characteristics. Two groups of samples were analyzed: first, during the confinement of five different breeds of steers and on their carcasses (abattoir A). Second, at the slaughter and processing of other groups of beef cattle (abattoir B). The Lm strains were serotyped with commercial antisera and by multiplex PCR, and subtyped by PFGE. No Lm was found among the 645 samples of feces, environment, feed and water during the confinement, but 13/48 of the refrigerated carcasses were contaminated, as well as the floor and the wall of the cold room at the abattoir A. Amongst the 516 samples of slaughter and processing environments, carcasses, utensils and equipment collected from abattoir B, 27 harbored Lm, being the majority from the c1ean area. Serotype 1/2b and 4b were the most frequent Lm serotypes in the carcasses of the steers in abattoir 1, and 1/2b and 1/2c in the abattoir B. The molecular typing by PFGE resulted in 15 Ascl and 13 Apal profiles, and 21 composite profiles, resulting in seven clonal groups. In these beef production chains the most important critical point for Lm contamination is the c1ean area of meat processing. Same clonal groups could be isolated in both abattoirs and in different areas on abattoir B, demonstrating high dissemination capability of these strains. Therefore, these results could aid the development of good manufacturing practices and HACCP, to prevent or eliminate the contamination for this pathogen...