RESUMO
Red-tail coral snake (Micrurus mipartitus) is a long and thin bicolor coral snake widely distributed in Colombia and is the coral that causes the majority of accidents in the Andean region, so it is important to keep this species in captivity for anti-venom production and research. However, maintaining this species in captivity is very difficult because it refuses to feed, in addition to the high mortality rate due to maladaptation syndrome. In this study a force feeding diet, diverse substrates for maintenance and a milking technique were evaluated. Additionally, individual variability of the venom was determined by High Performance Liquid Chromatography (HPLC), Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coagulant, Anticoagulant and Hemolytic activities. The results of this study demonstrate that it was possible to increase the survival rate of this species in captivity and to determine some of the important factors in the maintenance. As to the individual variability of the venom, we found differences in number and intensity of peaks recovered by chromatography and also displayed variations in some of its biological activities.
La coral "rabo de ají" es una coral bicolor larga y delgada. Esta especie está ampliamente distribuida en Colombia y es la coral que causa el mayor número de accidentes en la región Andina, por esto es importante mantener esta especie en cautiverio con fines de producción de antivenenos e investigación. No obstante, el mantenimiento de esta especie en cautiverio es difícil, debido a que se rehúsan a alimentarse voluntariamente y a que presentan alta mortalidad por el denominado síndrome de mal adaptación. En este estudio se evaluaron varios sustratos para el mantenimiento, además de una dieta forzada y una técnica de ordeño. Adicionalmente, se evaluó la variabilidad individual del veneno a través de cromatografía liquida de alta eficiencia (HPLC), electroforesis (SDS-PAGE) y las actividades coagulante, anticoagulante y hemolítica indirecta. Los resultados de este estudio demostraron que fue posible incrementar la sobrevivencia de esta especie en cautiverio, así como determinar algunos factores importantes en su mantenimiento. A partir de la evaluación del veneno se encontraron diferencias en el número y en la intensidad de picos en la cromatografía, así como en algunas de sus actividades biológicas.
RESUMO
El problema ético de la huelga de hambre ha dado cabida a soluciones contrapuestas, incluso en el contexto de teorías éticas que rechazan el suicidio. Algunos piensan que la sola honestidad del fin subjetivo basta para justificar la acción del huelguista. Otros, aunque son minoría, sostienen que la huelga de hambre es un acto reprobable per se, porque implica un atentado directo contra la vida o salud del sujeto. En este trabajo se defiende esta última interpretación. A juicio de los autores, la huelga de hambre es un caso de suicidio intencional. Luego, nunca es lícito, bajo ningún aspecto, llevar adelante una acción de esta naturaleza.
The ethical problem of hunger strikes has accommodated competing solutions, even in the context of ethical theories that reject suicide. Some believe the honesty of the subjective goal is enough in itself to justify the hunger striker's action. Others, although they are a minority, argue a hunger strike is a reprehensible act per se, because it implies a direct attempt on the person's life or health. This paper defends the second interpretation. According to the authors, a hunger strike is a case of intentional suicide. Therefore, it is never in any way licit to perform an act of this nature.
O problema ético da greve de fome tem proporcionado soluções contraditórias, inclusive no contexto das teorias éticas que rejeitam o suicídio. Alguns pensam que somente a honestidade do fim subjetivo basta para justificar a ação de quem faz a greve. Outros, embora sejam minoria, sustentam que a greve de fome é um ato reprovável por si porque implica um atentado direto contra a vida ou saúde do sujeito. Neste trabalho, defende-se esta última interpretação. No entender dos autores, a greve de fome é um caso de suicídio intencional. Portanto, nunca é lícito, sob nenhum aspecto, levar adiante uma ação dessa natureza.
Assuntos
Humanos , Suicídio , Saúde , Jejum , Vida , JulgamentoRESUMO
Objective To investigate intestinal bacteria genera distribution between normal weight and overweight/obesity school-age children in Yili region of Xinjiang.Methods Selecting eligible 150 fecal samples from school-age children (aged from 7 to 13 years old),all samples were divided into normal weight group and overweight/obesity group according to the body mass index (BMI),and each group contained 75 samples.Fecal samples were collected and DNA was extracted,then 6 types of intestinal bacteria genera were detected by the quantitative real-time polymerase chain reaction.Results The distribution of age,gender,ethnicity and hip circumference between 2 groups had no statistically significance (all P ＞ 0.05),except that of BMI and waist circumference (t ＝ 20.740,8.533,all P ＜ 0.01).The concentration of Lactobacillus,Clostridium and Enterococcus were significantly higher in the obese/overweight children (t =9.735,9.681,26.070,all P ＜ 0.01),whereas no significant differences were found in the concentration of Escherichia,Bifidobacterium and Bacteroides between 2 groups.According to the stratified analysis of ethnicity,gender and age,there was no significant difference among Han,Kazakh and Uyghur children.While stratified by gender,Bifidobactcrium was significantly lower in girls than that of boys (t =4.931,P ＜ 0.05).The distribution of the 6 types of intestinal bacteria genera was no statistically significant among different ethnicity,gender and age groups.Conclusions The intestinal bacteria genera number distribution in different ethnicity,gender and age groups makes no sense.The 16SrRNA type number of the Lactobacillus,Clostridium and Enterococcus may be associated with childhood obesity.