Formación de la cultura metrológica desde las potencialidades del deporte y la Educación Física

Este artículo trata la formación de la cultura metrológica, en los estudiantes de la Educación Preuniversitaria, desde las potencialidades del deporte y las clases de Educación Física, como uno de los elementos que debe ser priorizado en el proceso de enseñanza-aprendizaje de la Física y como parte de la preparación de los estudiantes para la vida. El objetivo de este trabajo consistió en diseñar un sistema de tareas docentes integradoras que permita aprovechar las potencialidades del deporte, en general, y las clases de Educación Física, en particular para la formación de la cultura metrológica en los estudiantes de décimo grado. Para el desarrollo de la investigación se utilizaron los métodos: analítico-sintético, inductivo-deductivo, modelación, observación, análisis documental, encuesta, entrevista y estadística descriptiva que permitieron procesar la información referente al tema objeto de investigación; así como determinar las causas de las insuficiencias que se presentan en el proceso de enseñanza-aprendizaje de la Física, para la formación de la cultura metrológica de los estudiantes de décimo grado. El sistema de tareas docentes integradoras propuesto se caracterizó por armonizar núcleos conceptuales básicos para el tratamiento a los contenidos metrológicos como son la estimación y medición de magnitudes y la conversión de unidades de medida; además de aprovechar las potencialidades del deporte y las clases de Educación Física.

Este artigo trata da formação da cultura metrológica em alunos da Educação Pré-Universitária, a partir das potencialidades das aulas de esportes e de Educação Física, como um dos elementos que devem ser priorizados no processo de ensino-aprendizagem de Física e como parte da preparação dos alunos para a vida. O objetivo deste trabalho foi conceber um sistema de tarefas didáticas integradoras que permita aproveitar as potencialidades do esporte, em geral, e das aulas de Educação Física, em particular, para a formação da cultura metrológica em alunos do décimo ano. Para desenvolver a pesquisa foram utilizados os seguintes métodos: analítico-sintético, indutivo-dedutivo, modelagem, observação, análise documental, levantamento, entrevista e estatística descritiva que permitiram o tratamento das informações referentes ao tema sob investigação; bem como determinar as causas das insuficiências que surgem no processo de ensino-aprendizagem de Física, para a formação da cultura metrológica dos alunos do décimo ano. O sistema de tarefas de ensino integrativo proposto caracterizou-se por harmonizar núcleos conceituais básicos para o tratamento de conteúdos metrológicos como a estimativa e medição de grandezas e a conversão de unidades de medida; além de aproveitar o potencial das aulas de esportes e Educação Física.

This article deals with the formation of metrological culture in Pre-University Education students, from the potential of sports and Physical Education classes, as one of the elements that must be prioritized in the teaching-learning process of Physics and as part of preparing students for life. The objective of this work was to design a system of integrative teaching tasks that allows taking advantage of the potential of sport, in general, and Physical Education classes, in particular, for the formation of metrological culture in tenth grade students. To develop the research, the following methods were used: analytical-synthetic, inductive-deductive, modeling, observation, documentary analysis, survey, interview and descriptive statistics that allowed the processing of information regarding the topic under investigation; as well as determine the causes of the insufficiencies that arise in the teaching-learning process of Physics, for the formation of the metrological culture of tenth grade students. The proposed system of integrative teaching tasks was characterized by harmonizing basic conceptual cores for the treatment of metrological content such as the estimation and measurement of magnitudes and the conversion of measurement units; in addition to taking advantage of potential of the sports and Physical Education classes.

Stimulation of mouse hematopoietic stem cells by angiogenin and DNA preparations

Immature hematopoietic progenitors are a constant source for renewal of hemocyte populations and the basic component of the tissue and cell repair apparatus. A unique property of these cells of internalizing extracellular double-stranded DNA has been previously shown. The leukostimulatory effect demonstrated in our pioneering studies was considered to be due to the feature of this cell. In the present research, we have analyzed the effects of DNA genome reconstructor preparation (DNAgr), DNAmix, and human recombinant angiogenin on both hematopoietic stem cells and multipotent progenitors. Treatment with bone marrow cells of experimental mice with these preparations stimulates colony formation by hematopoietic stem cells and proliferation of multipotent descendants. The main lineage responsible for this is the granulocyte-macrophage hematopoietic lineage. Using fluorescent microscopy as well as FACS assay, co-localization of primitive c-Kit- and Sca-1-positive progenitors and the TAMRA-labeled double-stranded DNA has been shown. Human recombinant angiogenin was used as a reference agent. Cells with specific markers were quantified in intact bone marrow and colonies grown in the presence of inducers. Quantitative analysis revealed that a total of 14,000 fragment copies of 500 bp, which is 0.2% of the haploid genome, can be delivered into early progenitors. Extracellular double-stranded DNA fragments stimulated the colony formation in early hematopoietic progenitors from the bone marrow, which assumed their effect on cells in G0. The observed number of Sca1+/c-Kit+ cells in colonies testifies to the possibility of both symmetrical and asymmetrical division of the initial hematopoietic stem cell and its progeny.

Curcumin plays an anti-osteoporosis role by inhibiting NF-κB signaling pathway to reduce oxidative stress damage to osteogenesis / 中国药理学通报

Aim To investigate the mechanism of curcumin inhibition of oxidative stress on osteogenic differentiation and its dose-dependent anti-osteoporosis effect. Methods Cellular oxidative stress models were used, different concentrations of curcumin were added to determinethebone formation markers, and the potential signaling pathways involvedwere detected. Meanwhile, the mouse model of osteoporosis ( ovariecto- mized, 0VX) was used to confirm its effect against osteoporosis. Results In vitro experiments found that low concentrations of curcumin (1-10 μmol · L

The lysine methyltransferase SMYD2 facilitates neointimal hyperplasia by regulating the HDAC3-SRF axis

Coronary restenosis is an important cause of poor long-term prognosis in patients with coronary heart disease. Here, we show that lysine methyltransferase SMYD2 expression in the nucleus is significantly elevated in serum- and PDGF-BB-induced vascular smooth muscle cells (VSMCs), and in tissues of carotid artery injury-induced neointimal hyperplasia. Smyd2 overexpression in VSMCs (Smyd2-vTg) facilitates, but treatment with its specific inhibitor LLY-507 or SMYD2 knockdown significantly inhibits VSMC phenotypic switching and carotid artery injury-induced neointima formation in mice. Transcriptome sequencing revealed that SMYD2 knockdown represses the expression of serum response factor (SRF) target genes and that SRF overexpression largely reverses the inhibitory effect of SMYD2 knockdown on VSMC proliferation. HDAC3 directly interacts with and deacetylates SRF, which enhances SRF transcriptional activity in VSMCs. Moreover, SMYD2 promotes HDAC3 expression via tri-methylation of H3K36 at its promoter. RGFP966, a specific inhibitor of HDAC3, not only counteracts the pro-proliferation effect of SMYD2 overexpression on VSMCs, but also inhibits carotid artery injury-induced neointima formation in mice. HDAC3 partially abolishes the inhibitory effect of SMYD2 knockdown on VSMC proliferation in a deacetylase activity-dependent manner. Our results reveal that the SMYD2-HDAC3-SRF axis constitutes a novel and critical epigenetic mechanism that regulates VSMC phenotypic switching and neointimal hyperplasia.

Effect of N-acetyl cysteine, rifampicin, and ozone on biofilm formation in pan-resistant Klebsiella pneumoniae: an experimental study

ABSTRACT BACKGROUND: To the best of our knowledge, this is the first study to evaluate the effectiveness of specific concentrations of antibiofilm agents, such as N-acetyl cysteine (NAC), rifampicin, and ozone, for the treatment of pan-resistant Klebsiella pneumoniae (PRKp). OBJECTIVES: We evaluated the effectiveness of antibiofilm agents, such as NAC, rifampicin, and ozone, on biofilm formation in PRKp at 2, 6, 24, and 72 h. DESIGN AND SETTING: This single-center experimental study was conducted on June 15, 2017, and July 15, 2018, at Istanbul Faculty of Medicine, Istanbul University, Turkey. METHODS: Biofilm formation and the efficacy of these agents on the biofilm layer were demonstrated using colony counting and laser-screened confocal microscopy. RESULTS: NAC at a final concentration of 2 μg/mL was administered to bacteria that formed biofilms (24 h), and no significant decrease was detected in the bacterial counts of all isolates (all P > 0.05). Rifampicin with a final concentration of 0.1 μg/mL was administered to bacteria that formed biofilm (24 h), and no significant decrease was detected in bacterial count (all P > 0.05). Notably, ozonated water of even 4.78 mg/L concentration for 72 h decreased the bacterial count by ≥ 2 log10. CONCLUSION: Different approaches are needed for treating PRKp isolates. We demonstrate that PRKp isolates can be successfully treated with higher concentrations of ozone.

Age estimation through the development of third molars in a case of multiple dismemberment / Estimación de la edad mediante el desarrollo de terceros molares en un caso de desmembramiento múltiple

El crimen organizado se ha convertido en un flagelo a nivel internacional conformado por grupos al margen de la ley que realizan todo tipo de actividades que involucran desde tráfico de personas, secuestros, extorsiones, narcotráfico y muchos otros delitos. Producto de este fenómeno, la desaparición y ejecución de personas es cada día más frecuente, en muchos casos los cuerpos son quemados o desmembrados para impedir o hacer más difícil la identificación. La odontología forense se ha convertido en una disciplina transcendental en la identificación de cadáveres y restos óseos, además de contar con múltiples métodos para estimar la edad aproximada de una persona. Se presenta el caso de un descuartizamiento múltiple de tres individuos masculinos donde era indispensable identificar si alguno correspondía a una persona menor de 18 años.

Organized crime has become an international scourge made up of outlaw groups that carry out all kinds of activities ranging from human trafficking, kidnapping, extortion, drug trafficking and many more. As a result of this phenomenon, the disappearance and execution of people is becoming more frequent every day, in many cases the bodies are burned or dismembered to prevent or make identification more difficult. Forensic odontology has become a transcendental discipline in the identification of corpses and skeletal remains, in addition to having multiple methods to estimate the approximate age of a person. The case of a multiple dismemberment of three male individuals is presented, where it was essential to identify a person under 18 years of age.

Linguagem enquanto Discurso conforme Michel Foucault: Uma revisão de literatura / El lenguaje como discurso desde Míchel foucault: una revisión delaliterarura / Language as Discourse according to Michel Foucault: Aliterature review

Este estudo se propõe a mostrar que o francês Michel Foucault, em todas as suas obras, oferece ferramentas para que sejam compreendidos, sob diferentes óticas, os problemas da sociedade por meio de interpretações sobre o viver humano. O objetivo dessa revisão de literatura é entender de que forma o discurso organiza as vozes dos sujeitos, como reelabora os discursos proferidos e os faz circular segundo Foucault. Apresenta-se conceitos básicos da Análise de Discurso iniciada na França por Michel Pêcheuxem 1969. O estudo percorre as obras em idas e vindas, com apoio em vários autores como Pêcheux e Mainguineauque ajudam na compreensãoe na aproximação entre método, teoria e prática.Conclui-se que a obra de Michel Foucault assinala que tudo o que é criado como saber oferece inúmeras possibilidades de recriação e está em permanente processo de transformação AU

Este estudio tiene como objetivo mostrar que el francés Michel Foucault, en todas sus obras, ofrece herramientas para comprender, desde diferentes perspectivas, los problemas de la sociedad a través de interpretaciones del vivir humano. El objetivo de esta revisión de la literatura comprender cómo el discurso organiza las voces de los sujetos, cómo reelabora los discursos pronunciados y los hace circular según Foucault. Presenta conceptos básicos del Análisis del Discurso iniciado en Francia por Michel Pêcheux en 1969. El estudio recorre las obras, con el apoyo de varios autores como Pêcheux y Mainguineau que ayudan a comprender y unir método, teoría y práctica. Se concluye que la obra de Michel Foucault destaca que todo lo creado como conocimiento ofrece innumerables posibilidades de recreación y se encuentra en un permanente proceso de transformación AU

This study aims to show that French Foucault, in all his works, offers tools to understand, from different perspectives, the problems of society, through interpretations of human living. The objective of this literature review is to understand how discourse organizes the subjects' voices, how it reworks the speeches given and makes them circulate according to Foucault. It presents basic concepts of Discourse Analysis initiated in France by Michel Pêcheux in 1969. The study goes back and forth through the works, with support from several authors such as Pêcheux and Mainguineau who help in understanding and bringing together method, theory and practice. It is concluded that Michel Foucault's work highlights that everything created as knowledge offers countless possibilities for recreation and is in a permanent process of transformation AU

Analysis of the concept of nurses' autonomy in intensive care units: A hybrid model / Análisis del concepto de autonomía de las Unidades de Cuidado Intensivo. Un modelo hídrido / Análise do conceito de autonomia das Unidades de Terapia Intensiva. Um modelo híbrido

Objective. To analyze the concept of autonomy of nurses in Intensive Care Units (ICU). Methods. The hybrid model approach proposed by Schwartz-Barcott and Kim, which includes theoretical, fieldwork and analytical phases, was used for this study. For the theoretical and fieldwork phases, the Graneheim and Lundman stages and the CORE-Q checklist were used, and the results were combined in the final analysis phase. For the theoretical phase, 46 related articles, two instruments and four books were identified after using a search strategy in 7 bibliographic databases in English and one in Persian with the terms MESH: 'nursing', 'autonomy' and 'intensive care'. The information extracted in the theoretical phase served as the basis for the design of the questions used in the semi-structured interviews in the fieldwork phase. Eight nurses with ICU experience working in hospitals affiliated to Isfahan University of Medical Sciences (Iran) participated in the fieldwork phase. Results. The antecedents of the concept of nurse autonomy in ICUs were: empowerment of the workforce, organizational platform, and social and individual views of the profession. Its attributes were professionalism and high personal capabilities. Finally, increased personal competencies, promotion of quality of care, improved attitudes towards the profession and professional outcomes were noted as consequences. Conclusion. The autonomy of nurses in the ICU can facilitate their empowerment, which translates into the promotion of their caring behaviours, followed by the improvement of patient outcomes and quality of care.

Objetivo. Analizar el concepto de autonomía entre las enfermeras de la Unidad de Cuidados Intensivos (UCI).Métodos. Para este estudio se utilizó el enfoque de modelo híbrido propuesto por Schwartz-Barcott y Kim que incluye las fases: teórica, de trabajo de campo y analítica. Para las fases teórica y de trabajo de campo se utilizaron las etapas de Graneheim y Lundman y la lista de comprobación CORE-Q, y los resultados se combinaron en la fase de análisis final. Para la fase teórica se identificaron 46 artículos relacionados, dos instrumentos y cuatro libros tras utilizar una estrategia de búsqueda en 7 bases de datos bibliográficas en inglés y otra en persa con los términos MESH: 'nursing', 'autonomy' e 'intensive care'. La información extraída en la fase teórica sirvió de base para el diseño de las preguntas que se utilizaron en las entrevistas semiestructuradas de la fase de trabajo de campo. En esta última fase participaron ocho enfermeras con experiencia en UCI que trabajaban en hospitales afiliados a la Universidad de Ciencias Médicas de Isfahan (Irán). Resultados. Los antecedentes del concepto de autonomía de las enfermeras en las Unidades de UCI fueron: el empoderamiento de la fuerza de trabajo, la plataforma organizativa y las opiniones sociales e individuales acerca de la profesión. Sus atributos fueron el profesionalismo y las grandes capacidades personales. Por último, se señalaron como consecuencias: el incremento de las competencias personales, la promoción de la calidad de los cuidados, la mejoría de las actitudes hacia la profesión y los resultados profesionales. Conclusión. La autonomía de las enfermeras en las UCI puede facilitar su empoderamiento, lo que se traduce en la promoción de sus conductas asistenciales, seguida de la mejora de los resultados de los pacientes y de la calidad asistencial.

Objetivo. Analisar o conceito de autonomia dos enfermeiros da Unidade de Terapia Intensiva (UTI). Métodos. Este estudo foi realizado utilizando a abordagem do modelo híbrido proposto por Schwartz- Barcott e Kim, que inclui as fases teórica, de campo e analítica. As etapas de Graneheim e Lundman e o checklist CORE-Q foram utilizados nas fases teórica e de trabalho de campo, e os resultados foram combinados na fase de análise final. Para a fase teórica, foram identificados 46 artigos relacionados, dois instrumentos e quatro livros após a utilização de uma estratégia de busca em 7 bases bibliográficas em inglês e outra em persa com os termos MESH: 'nursing', 'autonomy' e 'intensive care'. As informações extraídas na fase teórica serviram de base para o desenho das questões que foram utilizadas nas entrevistas semiestruturadas da fase de trabalho de campo. Participaram desta última fase, oito enfermeiras experientes em UTI trabalhando em hospitais afiliados à Universidade de Ciências Médicas de Isfahan, no Irã.Resultados. Os antecedentes do conceito de autonomia dos enfermeiros nas Unidades de Terapia Intensiva foram: o empoderamento da força de trabalho, a plataforma organizacional e as opiniões sociais e individuais sobre a profissão. Seus atributos eram profissionalismo e grandes habilidades pessoais. Por fim, foram apontadas as seguintes consequências: o aumento das competências pessoais, a promoção da qualidade dos cuidados, a melhoria das atitudes perante a profissão e os resultados profissionais. Conclusão. A autonomia dos enfermeiros na UTI pode facilitar seu empoderamento, que se traduz na promoção de seus comportamentos assistenciais, seguida da melhoria dos resultados dos pacientes e da qualidade da assistência.

Necesidades de formación de las personas editoras de la Universidad de Costa Rica

El crecimiento de la necesidad de información veraz ha llevado a que las revistas científicas se posicionen como medios de información confiables; lo cual hace que se requiera una necesidad de la profesionalización de esta labor que garantice la confiablidad y rigurosidad de las mismas. El objetivo de este manuscrito fue identificar las necesidades de formación de profesionales a cargo de la gestión editorial de las revistas científicas de la Universidad de Costa Rica. La investigación se realizó desde enero del 2019 hasta junio del 2020, por medio de un enfoque cualitativo de tipo descriptivo, con técnicas de recolección de este mismo enfoque. Se entrevistaron a las personas editoras y directoras de las principales revistas posicionadas en los primeros diez lugares del ranking UCR índex (a la fecha 4 de noviembre del 2019), se utilizó una entrevista semiestructurada con preguntas relacionadas a la identificación de actitudes, habilidades, conocimientos, necesidades de formación y el contexto en el que se desarrollan, estas representan las categorías de análisis utilizadas para el procesamiento de información. Entre los resultados se destacaron necesidades de formación en temáticas afines a los procesos administrativos relacionados con revistas, fundamentos de la gestión editorial, herramientas tecnológicas y el posicionamiento de las revistas; además se evidenciaron las cortas jornadas laborales que poseen los editores de revistas UCR. Se concluye que es imperativo que la UCR atienda estas necesidades de formación, además de fortalecer estos medios de divulgación del conocimiento y que se analice la oportunidad de ofrecer un programa de formación continua o bien una especialidad en gestión editorial.

The growth of the need for reliable information has led scientific journals to position themselves as reliable information media, which entails a need for the professionalization of this work that guarantees the reliability and rigor of this. The objective of this manuscript was to identify the training needs of the professionals in charge of the editorial management of the scientific journals of the University of Costa Rica. The research was carried out from January 2019 to June 2020, through a descriptive qualitative approach, with qualitative collection techniques. The editors and directors of the main journals positioned in the first ten places of the UCR index ranking (November 4th, 2019) were interviewed, a semi-structured interview was used with questions related to the identification of attitudes, skills, knowledge, training needs and the context in which they are developed, these represent the categories of analysis used for the processing of information. The main results shows that, training needs in topics related to the processes were highlighted, administrative issues related to journals, fundamentals of editorial management, technological tools and the positioning of journals; in addition, the short working hours that the editors of UCR magazines have were evidenced. It is concluded that it is imperative that the UCR meets these training needs, in addition to strengthening these means of disseminating knowledge and that the opportunity to offer a continuous training program or a specialty in editorial management be analyzed.

Variables, indicadores y criterios de evaluación del servicio médico como escenario de formación de especialistas

La calidad de la educación de posgrado concebida estratégicamente como la integración de la pertinencia social y la excelencia académica, deviene esencial en la gestión de las instituciones docente-asistenciales del sector salud. Sus múltiples dimensiones deben ser contextualizadas y son susceptibles de análisis y perfeccionamiento en cada servicio médico donde se desarrolla el proceso de formación de especialistas. Se utilizaron métodos teóricos y empíricos, para la identificación de las dificultades y la elaboración de la nueva propuesta. Se obtuvieron criterios sobre los servicios médicos como escenarios de formación, en los que se identificaron los problemas relativos a la actividad de posgrado. La propuesta consideró las directrices estratégicas, la población objeto de atención y los diferentes ámbitos temporales y espaciales identificados, con lo que pretende el fortalecimiento y desarrollo de la formación de posgrado en las Universidades de Ciencias Médicas en Cuba.

The quality of postgraduate education, strategically conceived as the integration of social relevance and academic excellence, becomes essential in the management of teaching-assistance institutions in the health sector. Its multiple dimensions must be contextualized and are susceptible to analysis and improvement in each medical service where the specialist formation process takes place. Theoretical and empirical methods were used, for the identification of the difficulties and the elaboration of the new proposal. Criteria on medical services were obtained as training scenarios, in which problems related to postgraduate activity were identified. The objective of the work is to expose a proposal of variables, indicators and evaluation criteria in the service as a scenario the specialist's formation. The proposal was considered the strategic guidelines, the target population and the different temporal and spatial areas identified, with which it intends to strengthen and develop postgraduate training in the Medical Sciences Universities in Cuba.

Narrativas sobre competição esportiva: reflexões e perspectivas / Narratives about sports competition: reflections and perspectives / Narrativas sobre la competición deportiva: reflexiones y perspectivas

Analisar as concepções sobre competição esportiva em publicações acadêmicas brasileiras é o foco deste trabalho. Utilizando-se da revisão sistemática, do tipo estado da arte, foram analisados 175 artigos no recorte temporal 2010-2020 (11 anos) em 14 revistas da área da Educação Física. Há parcimônia dos/as autores/as quanto a complexidade da competição e foram salutares indicativas do rememorar a essência do tema; independente da área de conhecimento específico, foram recorrentes indicativos de um processo respeitoso e pedagógico da competição aos praticantes. Ademais, confirmam que amadores e profissionais; homens e mulheres; idosos e jovens, todos podem (e devem) ter a opção da experiência esportiva no âmbito competitivo (AU).

Analyzing the concepts about sports competition in Brazilian academic publication is the objective of this paper. Using the state-of-art systematic review, 175 articles were considered from 14 Physical Education Journals from 2010 to 2020 (11 years). There is parsimony by the authors regarding the complexity of the competition and greetings indicative of remembering the essence of the theme; regardless of the area of specific knowledge, there were recurrent indicators of a respectful and pedagogical process of the competition for practitioners. Also, it was confirmed that amateurs and professionals; men and women; elderly and young; everyone can (and should) have the possibility of sporting experience in the competitive environment (AU).

Analizar las concepciones sobre la competición deportiva en las publicaciones académicas brasileñas es el foco de este trabajo. Utilizando la revisión sistemática del tipo estado del arte, se buscarán 175 artículos en el período de 2010-2020 en 14 revistas del área de Educación Física. Hay parcimônia a la complejidad de la competición y fueron saludos indicativos de recordar la esencia del tema; independientemente del área de conocimiento específico, fueron recurrentes indicativos para un proceso respetuoso y pedagógico de la competencia por practicantes. También confirman que los atletas aficionados y profesionales; hombres y mujeres; ancianos y jóvenes, todos pueden (y deben) tener la opción de la experiencia deportiva en el entorno competitivo

Effect and mechanism of serotonin-selective reuptake inhibitor on bone metabolism / 中国药房

Serotonin-selective reuptake inhibitor （SSRI） is the first-line drug for treating depression. SSRI mainly include fluoxetine， paroxetine， sertraline， fluvoxamine， citalopram and escitalopram， etc. SSRI has dual impact on bone metabolism. Short- term use of SSRI may have a positive impact on bone， but long-term use may lead to bone problems. This article summarizes the effects and mechanisms of SSRI on bone metabolism， indicating that SSRI can affect bone formation， bone resorption， mesenchymal stem cell differentiation， and regulate the expression of inflammatory cytokines. The impact of SSRI on bone metabolism can be achieved by affecting classical signaling pathways such as cAMP/PKA/CREB， Wnt/β-catenin， BMP/Smad， OPG/RANKL/RANK， and through centrally mediated effects.

Investigation on the antifungal activity of pyranium derivatives N2 / 药学实践杂志

Objective To study the antifungal activity of N2 derivatives. Methods The anti-fungal activity of N2 compounds was investigated by micro-liquid dilution. Then the activity of N2 compounds on hyphal and biofilm formation was investigated. Results N2 compounds had significant antifungal activity against Candida albicans. It also expressed actively inhibitory effect on hyphal and biofilm formation. The mechanism of its fungicidal function was to damage the structure of candida albicans’ cell membrane and cell wall. Conclusion The results showed that N2 had obvious antifungal activity against Candida albicans., which provided a new idea for the development of antifungal drugs and the solution of antifungal drugs resistance.

Comparative study of endoscopic spine surgery with and without assistance of an electromagnetic navigation surgical robot in the treatment of lumbar disc herniation / 中华显微外科杂志

Objective:To compair the clinical effect of endoscopic spine surgery with and without assistance of an electromagnetic navigation surgical robot(ENSR) system in the treatment of lumbar disc herniations(LDH).Methods:From March 2021 to December 2021, 73 patients with LDH underwent endoscopic minimally invasive treatment in the Department of Spine Surgery, Limin Hospital of Weihai High District. Thirty-eight patients were treated with endoscopic spine surgery assisted by domestic ENSR system(ENSR group), and 35 patients were treated with endoscopic spine surgery alone(endoscopic group). The patients received follow up regularly by telephone or Wechat. The intraoperative fluoroscopy times, puncture times, upper facet arthroplasty times, operation time, preoperative and postoperative Visual Analogue Scale(VAS), Japanese Orthopaedic Association Scores(JOA), and the MOS item short from health survey(SF-36)were compared between the 2 groups. The early postoperative efficacy was evaluated by modified MacNab criteria at 1 week after surgery. SPSS 26.0 was used to analyse the data. P<0.05 was considered that the difference was statistically significant. Results:The age, gender, body mass index(BMI) and other general data of the 2 groups were basically the same, and there was no statistically significant difference( P>0.05). There was no significant difference in the excellent and good rate of postoperative treatment between ENSR group(97.37%) and endoscopic group(94.29%)( Z=0.90, P>0.05). In terms of operation time, intraoperative fluoroscopy times, puncture times, and plasty times, ENSR group[(67.00±17.00) min, (4±2) times, 1 time, 1 time, respectively] was compared with the endoscopic group[(82.00±16.00] min, (17±6) times, (9±5) times, (5±2) times], and the difference was statistically significant( t=3.87,12.62, 9.87, respectively, P<0.05). There were significant differences in VAS, JOA and SF-36 scores between the 2 groups between before and after surgery( F=106.42, 112.14, 26.88, respectively, P<0.05). There was no significant difference in VAS, JOA and SF-36 scores before and after surgery between the 2 groups( F=0.95, 3.54, 0.97, respectively, P>0.05). Conclusion:The endoscopic spine surgery assisted by the ENSR can achieve satisfactory clinical results and is a safe and effective surgical assistance system. For the endoscopic surgery, assisted by the ENSR has obvious advantages in reducing the times of fluoroscopy, puncture, and facet arthroplasty, and shortening the operation time.

A new target for improving osteoporosis with eldecalcitol—Mini-modeling bone formation / 中华内分泌代谢杂志

As a basic anti-osteoporosis drug, active vitamin D mainly promotes intestinal absorption of calcium and phosphorus, inhibits the release of parathyroid hormone, and maintains normal blood calcium and phosphorus levels, thus ensuring bone health. Eldecalcitol(ED-71), a novel derivative of active vitamin D, was approved for clinical treatment of osteoporosis in Japan in 2011. Eldecalcitol not only improves intestinal calcium absorption and inhibits osteoclast differentiation, but also effectively promotes "mini-modeling" bone formation-focal bone formation that is independent of osteoclastic bone resorption, elevating trabecular continuity and increasing the thickness of weak trabeculae, thereby repairing the microinjury of bone tissue.

Melatonin attenuates bone resorption induced by acute sleep deprivation in growing rats / 中华内分泌代谢杂志

Objective:To explore the changes of bone turnover markers induced by sleep deprivation (SD) and the effect of melatonin supplementation on the bone turnover status.Methods:Six-week-old Wistar male rats were divided into SD, normal control (NC), and melatonin supplementation (SD+ MT) groups. Acute SD model was established using a modified multi-level bench method. The bone turnover markers, corticosterone, and melatonin in serum as well as Cathepsin K(CTSK) mRNA expression in bone tissue were tested.Results:Acute SD disrupted the balance between bone formation and bone absorption evidenced by rapid decreased serum procollagen type Ⅰ N-terminal propeptide (PⅠNP) levels and increased β cross-linked C-telopeptide of type Ⅰ collagen (β-CTX) levels ( P=0.003) from 24 h to 72 h. The exogenous melatonin treatment decreased β-CTX [(512.4±95.8) ng/mL vs (696.0±76.5) ng/mL, P=0.004] and the osteoclast-related gene CTSK mRNA level after 72 h SD. Conclusions:Acute SD accelerates bone resorption, which could be partially alleviated by melatonin supplementation.

Research progress on the formation mechanism of hepatocellular carcinoma with portal vein tumor thrombus / 中华肝胆外科杂志

Hepatocellular carcinoma is a common malignant disease in clinical practice, and portal vein tumor thrombosis (PVTT) is one of the important factors affecting the prognosis of hepatocellular carcinoma. PVTT has strong oncologic characteristics and is highly susceptible to extrahepatic metastasis, complicating portal hypertension, leading to gastrointestinal bleeding or liver failure and causing death. In this paper, we review the formation mechanism of hepatocellular carcinoma combined with PVTT in terms of local anatomy, hemodynamics, molecular biology and tumor microenvironment to provide effective reference for clinical treatment.

Innovative concept of following the "pain" to get the melon and its application in orthopedic trauma / 中华创伤杂志

Pain is a defensive response to body injury or potential injury, and is also the most common clinical manifestations in orthopedic trauma. Pain is critical to determine the causes of injury and therapeutic plans in the diagnosis and treatment of orthopedic trauma. Post-traumatic acute and chronic pain not only brings physical pain to patients, but also induces a variety of complications. Moreover, improper evaluation and management of pain can easily lead to missed diagnosis and misdiagnosis, thus prolonging the treatment cycle and affecting the quality of life. At present, the management of pain has no normative standard in the diagnosis and management of orthopedic trauma. Academician Zhang Yingze′s team has summarized the pain characteristics of various orthopedic trauma patients, traced back to the sources, and conduced the innovative concept of "pain" to get the melon to address issues such as insufficient pain assessment, incomplete etiology analysis and inadequate diagnostic thinking, hoping to achieve rapid and accurate diagnosis and early treatment. The authors elaborate on the connotation of the innovative concept of "pain" to get the melon and its application in clinical orthopedic trauma, aiming to explore the application value of this innovative concept, achieve rapid and accurate diagnosis of orthopedic trauma and provide a reference for formulating reasonable diagnosis and treatment programs.

Progress in risk factors for gallstone formation and prevention strategies for stony stage / 国际外科学杂志

Gallstone is a common and frequent disease and frequent incidence, secondary infection and cancer seriously affect the health of patients. Academic organizations in different regions have issued multiple guidelines and consensus to promote the normative diagnosis and treatment of gallstones. However, in clinical practice, most symptomatic gallstones are treated, while the formation and prevention process of gallstones are ignored, making the concept of treating without a disease has not been strengthened.This article reviews the risk factors and mechanisms of gallstone formation, and points out the importance of effective prevention during stone formation. In the stage of gallstone formation, the high risk factors of stone formation can be analyzed through two aspects of injury factors and protective factors, and the high risk groups of stone formation can be screened out. According to the pathophysiological progression of gallstones, personalized prevention and follow-up strategies can be developed for the stone formation stage of gallstones.

Pinpointing Synechococcus Rubisco large subunit sections involved in heterologous holoenzyme formation in Escherichia coli

Aims@#Heterologous holoenzyme formation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) has been a challenge due to a limited understanding of its biogenesis. Unlike bacterial Rubiscos, eukaryotic Rubiscos are incompatible with the Escherichia coli (E. coli) chaperone system to fold and assemble into the functional hexadecameric conformation (L8S8), which comprises eight large subunits (RbcL) and eight small subunits (RbcS). Our previous study reported three sections (residues 248-297, 348-397 and 398-447) within the RbcL of Synechococcus elongatus PCC6301, which may be important for the formation of L8S8 in E. coli. The present study further examined these three sections separately, dividing them into six sections of 25 residues (i.e., residues 248-272, 273-297, 348-372, 373-397, 398-422 and 423-447).@*Methodology and results@#Six chimeric Rubiscos with each section within the RbcL from Synechococcus replaced by their respective counterpart sequence from Chlamydomonas reinhardtii were constructed and checked for their effect on holoenzyme formation in E. coli. The present study shows that Section 1 (residues 248-272; section of Synechococcus RbcL replaced by corresponding Chlamydomonas sequence), Section 2 (residues 273-297), Section 3 (residues 348-372) and Section 6 (residues 423-447) chimeras failed to fold and assemble despite successful expression of both RbcL and RbcS. Only Section 4 (residues 373-397) and 5 (residues 398-422) chimeras could form L8S8 in E. coli.@*Conclusion, significance and impact of study@#GroEL chaperonin mediates the folding of bacterial RbcL in E. coli. Therefore, residues 248-297, 348-372 and 423-447 of Synechococcus RbcL may be important for interacting with the GroEL chaperonin for successful holoenzyme formation in E. coli.