Identification of 45 Kinds of Chemical Components of Forsythia suspensa by UPLC-Q-TOF-MS / 中国药房

OBJECTIVE： To identify chemical components of Forsythia suspense. METHODS： UPLC-Q-TOF-MS technology was used for the chemical components analysis of F. suspense. The determination was performed on ACQUITY UPLC BEH C18 column with mobile phase consisted of 0.1％ formic acid aqueous solution （A）- 0.1％ formic acid acetonitrile solution （B） with  gradient elution， at the flow rate of 0.3 mL/min； the column temperature was set at 30 ℃； the sample size was 5 μL. Positive and negative ions were detected by electrospray ionization. The temperature of the ion source was 550 ℃； the atomizing gas was N2； the atomizing gas and the auxiliary pressure were 379.2 kPa； the air curtain pressure was 241.3 kPa； the decluster voltage was 80 V/－80 V； the collision energy was 35 eV/－35 eV； the mass scanning range was 80-1 500 Da. Peakview 2.0 software was used to screen the target components by the first-order mass spectrometry， and calculate the high-resolution and accurate molecular weight of each component， compare with the reference spectrum and related literature， or calculate the elemental composition of fragment ions in the second-order mass spectrometry， analyze their decomposition pathways， then infer the structure of compounds. RESULTS & CONCLUSIONS： 45 kinds of compounds were identified from F. suspense，which included 7 phenylethanol glycosides，5 lignans，5 terpenes， 12 flavonoids，7 organic acids，2 phenols，2 quinones，2 glycosides and 3 other components. There were 19 compounds identified for the first time in F. suspense. The study provides a reference for the in-depth study of the pharmacodynamic substance basis of F. suspense and the rapid qualitative and quantitative analysis of the components.

Optimization of the Molding Technology of Forsythia Suspense Leaves Healthy Instant Tea / 中国药师

Objective:To determine the optimal molding technology of Forsythia Suspense leaves healthy instant tea. Methods:The effects of the ratio of different excipients to dry extract powder and the concentration of wetting agent on the indices including dis-solubility, appearance and formability were investigated by single factor tests. The drying time was determined with moisture as the in-dex, and the final forming process was optimized. Results:The optimal molding progress was as follows:the ratio of dry extract powder to lactose was 1 :1. 5, and after mixed completely, 80% ethanol was used as the wetting agent to prepare wet granules, finally dried at 60℃ for 1. 5 h. Conclusion:The molding technology of Forsythia suspense leaves healthy instant tea is feasible, which can provide ref-erence for the comprehensive development and utilization of Forsythia Suspense leaves.

Optimization of the Molding Technology of Forsythia Suspense Leaves Healthy Instant Tea / 中国药师

Objective:To determine the optimal molding technology of Forsythia Suspense leaves healthy instant tea. Methods:The effects of the ratio of different excipients to dry extract powder and the concentration of wetting agent on the indices including dis-solubility, appearance and formability were investigated by single factor tests. The drying time was determined with moisture as the in-dex, and the final forming process was optimized. Results:The optimal molding progress was as follows:the ratio of dry extract powder to lactose was 1 :1. 5, and after mixed completely, 80% ethanol was used as the wetting agent to prepare wet granules, finally dried at 60℃ for 1. 5 h. Conclusion:The molding technology of Forsythia suspense leaves healthy instant tea is feasible, which can provide ref-erence for the comprehensive development and utilization of Forsythia Suspense leaves.

Simultaneous quantification of eight constituents in the seed of Forsythia suspense (Thunb.) Vahl by HPLC-MS/MS method / 中草药

Objective: To develop an HPLC-MS/MS method for the simultaneous determination of forsythoside A, phillyrin, forsythiaside, rutin, quercetin, isoquercitrin, ferulic acid, and hesperidin in the seed of Forsythia suspense (Thunb.) Vahl. Methods: Chromatographic separation was performed on a Diamonsil C18 column (150 mm × 4.6 mm, 5 μm) with linear gradient elution of methanol and 0.1% formic acid at a flow rate of 800 μL/min, and the injection volume was 10 μL. Multiple-reaction monitoring (MRM) was employed with switching electrospray ion source polarity in negative mode. The ion spray voltage was set at-4 500 V and the turbo spray temperature was maintained at 650℃. Results: All calibration curves showed good linearity within the test ranges. The average recoveries of the compounds ranged from 98.1% to 101.8%. The precisions (RSD) for the investigated components were less than 0.84%. The contents of forsythoside A, phillyrin, forsythiaside and rutin are higher than quercetin, isoquercitrin, ferulic acid and hesperidin. And the average contents of forsythoside A, phillyrin, forsythiaside and rutin are 1 609.78, 80.08, 86.64 and 373.86 μg/g, respectively. Conclusion: An efficient, rapid, and sensitive method is first established for the qualitation and quantification of eight major components in the seed of Forsythia suspense (Thunb.) Vahl. The satisfactory results demonstrate that the method could be applied as a reliable quality control method for the seed of Forsythia suspense (Thunb.) Vahl.

Herbal textual research of common Mongolian medicine "Du Ge Mo Nong" / 中国中药杂志

This study is aimed to provide the source for clinical medication by clarifing the common base of Mongolia medicinal materials "Du Ge Mo Nong". In this paper literature research and plant taxonomy method were adopted combined with investigation of the markets and clinical use status to clarify the origin of common traditional Mongolian medicine "Du Ge Mo Nong". The results showed that the Mongolian medicine "Du Ge Mo Nong" and Tibetan medicine "Du Mu Niu" were the same crude drug and originated from the seeds of Holarrhena antidysenteriaca of family Apocynaceae in earliest time. In Mongolian medicine clinic, the seed of Cynanchum thesioides of family Asclepiadaceae and the fruit of Forsythia suspense of family Oleaceae was used respectively about 18 century and recent time, as the substitutes of H. antidysenteriaca. In present standards on Mongolian material medicine, C. thesioides is including only, and H. antidysenterica is not used almost. In Tibetan medicine clinic, H. antidysenterica is being used so far. But there are various substitutes including the seeds, fruits or grass of many species classified to family Apocynaceae, Asclepiadaceae and Onagraceae from ancient time to the present, and C. forrestii and C. komarovii may be used at present. It's necessary to strengthen the arrangement on Mongolian medicine's varieties by the multidisciplinary methods including literature research, investigation of resources and the use state, and comparison of active substances and biological activities between the different substitutes,and so on.

Chemical constituents in fruits of Forsythia suspensa / 中草药

Objective: To study the chemical constituents from the fruits of Forsythia suspensa. Methods: Compounds were isolated and purified by silica gel chromatography, repeated recrystallization, and other methods, and their structures were identified by spectral analysis. Results: Eleven compounds were isolated from the fruits of F. suspensa and were identified as acetyloleanolic acid (1), isobauerenyl acetate (2), β-amyrin acetate (3), cleroindicin A (4), 1-methyl-β-D-glucopyranoside (5), esculentic acid (6), asiatic acid (7), forsythin (8), oleanolic acid (9), β-sitosterol (10), and β-daucosterol (11). Conclusion: Compounds 1 and 4-7 are obtained from the plant of Forsythia Vahl for the first time.

The effects and mechanisms of Forsythia suspense on the expression of Foxp3 on splenocytes and level of Treg in peripheral blood in severely burnt rats / 细胞与分子免疫学杂志

AIM: To explore the immunity modulation function of aqueous of Forsythia suspense (AFS) and its possible mechanisms. METHODS: Rats of burned model group were burned with vapor under 3mpa pressure and 108℃ temperature for 8 seconds to achieve deep partialthickness bum, to make a thirty percent total body surface area (TBSA)bum. The experiment were divided into five groups: Control group: without any treatment; 8 PBH group: 8 h after burn; the rats of AFS1 guoup, AFS2 group and AFS3 group of them were given AFS 5 g/kg, 2.5 g/kg, 1.25 g/kg once a day by Po. pathway for seven days before burns, respectively. Rats were sacrificed before and 8h after burn, The percentage of Treg cells in CD4~+ T cells was detected by flow cytometry(FCM) ; the expression of Foxp3 mRNA on splenocytes were measured by RT-PCR, and the protein of Foxp3 activity was evaluated by immunohistochemistry staining. RESULTS: Compared with Control group, the expression of Foxp3mRNA and protein on the splenocytes were upregulated markedly(P <0.01), and the percentage of Treg were significantly increased (P < 0.01) in the 8PBH group. AFS1, AFS2 and AFS3 significantly attenuated these increases (P < 0.01), which was dose-dependent. CONCLUSION: AFS has immunity modulation function and mechanism of it is corrected with Foxp3 mRNA on splenocytes.

Study of Drying Methods of Forsythia Suspense Leaves / 中国中医药信息杂志

Objective Better drying methods of Forsythia suspense leaves was studied to provide some basis for the development and utilization of Forsythia suspense leaves. Methods A RP-HPLC method was used on XTerraTMRP8 (3.9 mm?150 mm, 5 ?m) column with acetonitrile-0.1% acetic acid solution (12 : 88) as mobile phase for forsythoside. The detection wavelength of forsythoside was at 328 nm and detection temperature was 30 ℃. Results The contents of forsythoside was higher when dry, microwave drying, absorbent paper drying, steaming 2～10 min, cooking 2～10 min was used to deal with Forsythia suspense leaves. The contents of forsythoside was lower when dried, drying, infrared drying, vacuum drying was used to deal with Forsythia suspense leaves. Conclusion Steaming 2～10 min, cooking 2～ 10 min is more appropriate for drying of Forsythia suspense leaves, when factors of the contents of forsythoside, the cost and ease of operation were considerd.

Observation of Antibacterial Activity of Galla Chinensis etc. 14 Kinds of Traditional Chinese Medicine against 112 Strains of Staphlococcus Aureus in Vitro / 中国中医药信息杂志

Objective To evaluate the antibacterial activity of Galla chinensis etc.14 kinds of TCM against 112 strains of Staphylococcus aureus in vitro. Method Deterimination of antibacterial activity of Galla chinensis etc.against 112 strains of Staphylococcus aureus was performed by using new agar dilution method. Results The antibacterial efficiency of Galla chinensis, Forsythia suspensa and Acacia catechu (L.) Willd against 112 strains Staphylococcus aureus was good, and the MIC90 was 0.102, 0.244, 1.19 mg/mL respectively. Conclusion Galla chinensis etc. 14 kinds of TCM had strong antibacterial activity agsinst 112 srains of Staphylococcus aureus.

STUDY ON ANTI-SENILE AND ANTI-OXIDATIVE ACTIVITIES OF FORSYTHIA SUSPENSE LEAVES TEA / 营养学报

Objective: To study the anti-senile and anti-oxidative activities of Forsythia suspense leaves tea(FSLT) in mice. Methods: 36 ICR mice(7 mo age) were randomly divided into three groups: senior control group,ig distilled water ; FSLT1 group,ig FSLT 0.3 g ?kg-1?d-1 ; and FSLT2 group,ig FSLT 0.9 g?kg-1?d-1 .The other 12 ICR mice(young control group) were given ig distilled water. MDA and MAO-B in brain and mitochondria were measured. SOD,MDA and POD in heart, liver index, thymus index and spleen index were determined. Results: Forsythia suspense leaves tea may reduce the level of MDA and MAO-B, and increase the activities of SOD and POD. Conclusion: Forsythia suspense leaves tea may have anti-senile and anti-oxidative activities.