RESUMO
OBJECTIVE: To identify the main chemical constituents in the extract of deep fried strychnas by UPLC-Q-TOF-MS. METHODS: Agilent SB-C18(2.1 mm×100 mm, 1.8 μm) column was used. Gradient elution was conducted with mobile phase consisting of 0.1% formic acid solution (A) and acetonitrile (B)at a flow rate of 0.2 mL•min-1. The column temperature was maitained at 35 ℃. MS analysis was based on information associated mode (IDA) that positive and negative ions were respectively collected. RESULTS: A total of 31 compounds were identified in deep fried strychnas, of which eight had not been reported as Semen Strychni,and four new compounds were found in the positive and negative ion mode. The main chemical constituents included alkaloids, organic acids, glycosides, etc. CONCLUSION: The method is accurate, reliable, and efficient, and is suitable for rapid identification of the ingredients in deep fried strychnas, which provides a reference for the development and utilization of processed products of Semen Strychni and clarification of its efficacy and material basis.
RESUMO
From theYellow Emperorand other representative Chinese classics of the Tang and its previous dynasties we assorted the contents of extracting solvent, boiling time, boiling heat and special frying methods. By studying these contents, we found that immersion can reduce side effects and improve efficacy;boiling solvents include water, vinegar and so on;6-8 times the amount of water than the drug is preferred, boiling times is usually once;special frying methods include decoction first, decoction later, decocted medicine water used in place of water as solvent, infused decoction, etc. Decoctions were generally not allowed to stand overnight. TCM decocting methods had great impacts on its effectiveness. It can provide more medication ideas for doctors and provide boiling guidance to patients.