RESUMO
Leaf blight outbroke in Rehmannia glutinosa plantation in Wenxian county, Henan province in 2019. R. glutinosa plants with diseased leaves were collected from the plantation, and three strains were isolated from the diseased leaf samples. Pathogenicity test, morphological observation, and phylogenetic analysis of ITS, EF1-α, and Tub suggested that they were respectively Fusarium proliferatum, F. oxysporum, and F.acuminatum. Among them, F. acuminatum, as a pathogen of R. glutinosa leaf disease, had never been reported. To clarify the biological characteristics of F. acuminatum, this study tested the influence of light, pH, temperature, medium, carbon source, and nitrogen source on the mycelial growth rate of the pathogen during a 5-day culture period, and explored the lethal temperature. The results showed that the mycelia grew well under the photoperiod of 12 h light/12 h darkness, at 5-40 ℃(optimal temperature: 25 ℃), at pH 4-11(optimal pH: 7.0), on a variety of media(optimal medium: oatmeal agar), and in the presence of diverse carbon and nitrogen sources(optimal carbon source: soluble starch; optimal nitrogen source: sodium nitrate). The lethal temperature was verified to be 51 ℃(10 min). The conclusion is expected to lay a scientific basis for diagnosis and control of R. glutinosa leaf diseases caused by F. acuminatum.
Assuntos
Carbono , Nitrogênio , Filogenia , RehmanniaRESUMO
ABSTRACT Dye stuff released to the ecosystem from textile industries cause a serious contamination and become a major environmental problem over the last few decades. As biological decolorization of textile wastewater is an important issue, Fusarium . acuminatum was used to removal of a frequently used textile dye, methyl orange. Live pellet of Fusarium acuminatum was used and decolorization studies performed in various temperatures and pH conditions with different dye concentrations. The highest decolorization rate was observed at 35ᴼC. 60 mg/L was found as the optimum initial dye concentration. In the pH range of 3-4, decolorization rate was approximately 70%. It was seen that Fusarium acuminatum have the great ability of the methyl orange removal. To our knowledge, it took place for the first time in the literature.