Identification of Bacterial Isolates and Their Antimicrobial Susceptibility Pattern from Wound/Pus Sample in a Tertiary Care Hospital, Gwalior, India

Objective: The goal of this investigation was to look at the frequency and dispersal of bacteria isolated from pus/wound, as well as their susceptibility patterns. Materials and Methods?A study was conducted on 175 patients who provided pus and/or wound discharge samples in different wards (outpatient department or inpatient department). MacConkey agar and blood agar plates were immediately inoculated with samples and incubated at 37°C for 24?hours. The Gram stain and biochemical tests were used to identify all isolates after incubation. Kirby–Bauer's disc diffusion method was used to perform sensitivity tests on Mueller–Hinton agar plates. Results?This study covered 175 patients, with a bacterial isolation rate of 102 (58.28%). Males outnumbered females in the samples (M:F?=?1.8:1), with a median age of 45 years as majority were in the age group of 40 to 60 years which was 41 (40.20%). Total 90.1% samples showed monomicrobial infection, whereas 9.8% showed polymicrobial infection, and total 112 bacterial strains were isolated. Conclusion?Escherichia coli was the most prevalent isolate in present investigation, followed by Pseudomonas aeruginosa. Chloramphenicol is the only antibiotic which is effective for both gram-negative bacilli and gram-positive cocci. This report's susceptibility statistic may be worth considering for developing empiric treatment regimens for pyogenic infections.

A comprehensive update: gastrointestinal microflora, gastric cancer and gastric premalignant condition, and intervention by traditional Chinese medicine / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

With the recent upsurge of studies in the field of microbiology, we have learned more about the complexity of the gastrointestinal microecosystem. More than 30 genera and 1000 species of gastrointestinal microflora have been found. The structure of the normal microflora is relatively stable, and is in an interdependent and restricted dynamic equilibrium with the body. In recent years, studies have shown that there is a potential relationship between gastrointestinal microflora imbalance and gastric cancer (GC) and precancerous lesions. So, restoring the balance of gastrointestinal microflora is of great significance. Moreover, intervention in gastric premalignant condition (GPC), also known as precancerous lesion of gastric cancer (PLGC), has been the focus of current clinical studies. The holistic view of traditional Chinese medicine (TCM) is consistent with the microecology concept, and oral TCM can play a two-way regulatory role directly with the microflora in the digestive tract, restoring the homeostasis of gastrointestinal microflora to prevent canceration. However, large gaps in knowledge remain to be addressed. This review aims to provide new ideas and a reference for clinical practice.

Prospect of Clinical Application of Glypican-3 Targeted Therapy for Hepatocellular Carcinoma / 中国生物化学与分子生物学报

Glypican-3 (GPC3) is an oncofetal protein anchored on the plasma membrane and highly expressed in hepatocellular carcinoma (HCC).GPC3 could he used as a biomarker for the diagnosis of HCC and the serum levels of GPC3 in liver cancer patients has a significant role for their prognosis.Moreover, GPC3 in HCC cells is immunoreactive, rendering it a suitable target for the treatment of HCC.Nowadays, several clinical trials targeting GPC3 for HCC therapy have already been conducted: new anti- GPC3 antibodies are generated; the clinical trials about its combination administration with other targeted medicines are being in progress; (tP(3-targeted TRAB, GPC3 vaccines and GPC3-based chimeric antigen receptor T-cells (CAR-T) therapy are under investigation.In this review, we briefly discuss the structure of GPC3, its role in HCC pathogenesis and summarize the recent development in the clinical application of GPC3.We firmly believe that GPC3 would be a promising target for HCC therapy.And further studies focusing on GPC3 should provide us more solid evidence.

Preparation of anti-GPC3 single chain antibody for targeted detection of hepatocellular carcinoma / 生物工程学报

Glypican-3 (GPC3) is a key member of Glypican family and plays an important role in the development, angiogenesis and metastasis of hepatocellular carcinoma (HCC). Most HCC overexpresses GPC3, but GPC3 is hardly detected in normal adult liver and benign liver lesions, so it is regarded as a highly specific diagnostic marker and an ideal therapeutic target for HCC. In this study, we cloned the heavy and light chain variable region gene from the monoclonal antibody targeted to GPC3 screened in the previous stage, and linked it with a segment of flexible peptide (Linker) to obtain the single chain antibody against GPC3. The single chain antibody gene was cloned into vector for prokaryotic expression and purified to obtain high purity protein. Detection shows that the single-chain antibody produced by us has the same binding activity with the full-length antibody, and can accurately target the tumor site of Huh7 tumor-bearing model mice after coupling Cy5.5 fluorescence, suggesting that the single-chain antibody has the potential to realize multi-directional liver cancer precise surgical navigation under the guidance of a probe.

Correlation Between Expression Levels of Copper Chaperone for Superoxide Dismutase and Malignancy Related Biomarkers in Hepatocellular Carcinoma / 中山大学学报(医学科学版)

@#【Objective】To explore the correlation between the expression levels of copper chaperone for superoxide dismutase（CCS）and the malignancy related biomarkers in hepatocellular carcinoma（HCC）.【Methods】From January to December 2018，we obtained fresh samples of surgically dissected HCC paired with para-carcinoma normal tissues from 10 HCC patients and collected their clinical and pathological data. Western blotting（WB）was performed to examine the expression of CCS in HCC tissues and adjacent noncancerous tissues. Immunohistochemical staining（IHC）was employed to detect the expression of Ki67，CD34，vimentin and glypican-3（GPC3）. The correlation between the expression levels of CCS and biomarkers was analyzed by using Wilcoxon rank sum test. The association between CCS expression and clinical pathological characteristics of HCC patients was investigated by using Fisher′s exact probability test.【Results】In 7 of the 10 HCC cases，the expression level of CCS in HCC tissue samples was lower than that in adjacent noncancerous tissues，and in other 3 HCC cases，CCS expression higher. In the group with low CCS expression，compared with those in the group with high CCS expression，the expression levels of Ki67，vimentin and GPC3 were higher （Z=- 2.400，P=0.016；Z=- 2.423，P=0.015；Z=- 2.400，P=0.016），while the expression level of CD34 lower（Z=- 2.423，P=0.015）. There was no statistically significant difference in clinical and pathological variables including gender ，age ，hepatitis B virus infection，liver cirrhosis，preoperative serum AFP level，tumor size，Edmondson-Steiner grade and microvascular invasion between two groups with high and low CCS expression.【Conclusions】The results revealed that in most of HCC patients，the expression level of CCS in HCC tissues was lower than that in adjacent noncancerous tissues. Additionally ， higher expression levels of Ki67，vimentin and GPC3 in HCC tissues with low CCS expression indicated that low expression level of CCS correlated with malignant biological behaviors such as HCC proliferation ，invasion and metastasis. The mechanism that the expression level of CD34 appeared lower in HCC tissues with low CCS expression，however，needs further study. These findings suggest that compared with that in normal liver tissues，CCS expression is decreased in a majority of the cases，and it may serve as a promising therapeutic and prognostic biomarker for HCC.

Clinicopathological characteristics of gastric cancer with enteroblastic differentiation / 中国肿瘤临床

Objective: To investigate the clinicopathological features and molecular phenotypes of gastric cancer with enteroblastic dif-ferentiation (GCED). Methods: A retrospective analysis of 337 patients with gastric adenocarcinoma diagnosed by the pathology de-partment of the First Affiliated Hospital of Zhejiang University in March 2013-2017 was conducted. Of them, 8 patients were diag-nosed with gastric carcinoma with intestinal blastocyte differentiation. All the patients were elderly, including 6 men and 2 women. The onset age was 68-83 years (mean 76.6 years). Two cases had serum AFP≥200 μg/L before treatment. According to the histopatho-logical morphology, the immunophenotype was analyzed by immunohistochemistry, the SALL4 gene was detected using reverse tran-scription-polymerase chain reaction (RT-PCR), and the relevant literature was reviewed. Results: Microscopically, all cases had primi-tive enteroid structures, consisting of cubic or columnar cells with clear cytoplasm, and immunohistochemical staining showed positivi-ty for either AFP and GPC3 or SALL4. The expression of SALL4 mRNA was significantly increased by RT-PCR. Follow-up from 1 to 5 years showed that 5 patients had liver and other organ metastases, 2 patients died, and 1 patient survived without a tumor. Conclusions:GCED is a rare invasive gastric adenocarcinoma with a worse prognosis than that of normal intestinal adenocarcinoma. The treatment of general intestinal adenocarcinoma has little effect. There are some characteristic changes in histology. It would be helpful for diag-nosis and differential diagnosis if clinicians are familiar with the tumor spectrum and genetic characteristics. Target therapy for an origi-nal marker, such as SALL4, has a bright future.

Evaluación de la calidad de las guías de práctica clínica utilizadas en la rehabilitación del accidente cerebrovascular / Evaluation of the quality of the clinical practice guidelines used in the rehabilitation of stroke

Contexto: las guías de práctica clínica (GPC) proporcionan recomendaciones sistemáticas para facilitar la toma de decisiones en base a la mejor evidencia científica. Objetivo: evaluar la calidad de guías de práctica clínica relacionadas a rehabilitación del accidente cerebrovascular (AVC). Métodos: se realizó una búsqueda en línea de GPC sobre rehabilitación del ACV en base al instrumento AGREE II que garantiza la calidad académica; al ser una herramienta informática, valora el rigor metodológico y la transparencia en el desarrollo de las GPC. Resultados: mediante buscadores, se seleccionaron 63 guías de manejo del ACV para evaluación, descartándose documentos en idioma anglosajón (n=13) y documentos no técnicos (n=29)- Luego del primer tamizaje, se eliminaron 18 guías adicionales por no sujetarse a los criterios de inclusión: a) idioma español, b) año de publicación entre enero de 2012 a marzo de 2017, c) texto completo, d) acceso libre a texto completo y e) vinculadas a la rehabilitación del ACV. Apenas tres guías cumplieron la totalidad de criterios de inclusión. Los resultados del AGREE II variaron ampliamente entre los distintos dominios otorgándose el mayor puntaje a una guía que cumplió el 60% de cada uno de los seis dominios valorados. Conclusión: existe variabilidad en la calidad de las guías evaluadas; por calidad metodológica fue seleccionada la guía publicada por el Ministerio de Salud y Protección Social COLCIENCIA de Colombia. Se incrementarse la rigurosidad al momento de desarrollar guías de manejo, bajo parámetros de escritura, metodología y evidencia científica. (AU)

Context: clinical practice guidelines (CPG) provide systematic recommendations to facilitate decision making based on the best scientific evidence. Objective: to evaluate the quality of clinical practice guidelines related to stroke rehabilitation (CVA). Methods: an online CPG search was conducted on ACV rehabilitation based on the AGREE II instrument that guarantees academic quality; being a computer tool, it values methodological rigor and transparency in the development of CPGs. Results: through search engines, 63 guidelines were selected for evaluation, discarding documents in the Anglo-Saxon language (n = 13) and non-technical documents (n = 29) - After the first screening, 18 additional guides were eliminated due to not being subject to the inclusion criteria: a) Spanish language, b) year of publication between January 2012 to March 2017, c) full text, d) free access to full text and e) related to the rehabilitation of the ACV. Only three guides met all the inclusion criteria. The results of AGREE II varied widely among the different domains, with the highest score being given to a guide that met 60% of each of the six domains assessed. Conclusion: there is variability in the quality of the evaluated guides; the guide published by the Ministry of Health and Social Protection COLCIENCIA of Colombia was selected for methodological quality. Increased rigor at the time of developing management guides, under writing parameters, methodology and scientific evidence. (AU)

Roles of exosomes in the diagnosis and treatment of patients with pancreatic cancer / 中华肝胆外科杂志

Exosomes are small membrane bound vesicles typically 30 ～ 150 nm in size which are widely distributed in the body fluids.The cytosol is rich in proteins and nucleic acids which mediates communication between different cells and transporting substances.Exosomes are involved in pancreatic cancer initiation and progression by promoting the formation of tumor microenvironment,enhancing gemcitabine resistance.GPC1 + circulating exosomes and high exosome levels of microRNA-10b serve as potertial non-invasive diagnostic tools to detect pancreatic cancer in the early stage.Meanwhile,exosomes could successfully deliver genetic drugs,anticancer drugs to target cells.In this article,we provide a review on the recent advances on exosomes in the diagnosis and treatment of pancreatic cancer.

Effect of targeted ultrasound microbubble carrying target gene on the proliferation of HepG2 cells in human hepatocellular carcinoma / 重庆医学

Objective To prepare a targeted ultrasound micro bubble,which carried the HSV-TK gene,and investigate the in vitro target searching ability of the micro bubbles and inhibitory effect on the proliferation of HepG2 cells.Methods Ultrasonic micro bubbles were prepared by mechanical vibration method,construction of targeted HSV-TK ultrasound micro bubbles by biotin affinity bridge construction.To detect the general characteristics of ultrasound micro bubbles,and to test its effect on the proliferation of HepG2 cells in vitro.Results HSV-TK targeted ultrasound microbubbles more gathered on the surface of HepG2 cells,through detection of PCNA and MTT,it was found that the proliferation of gene targeting microbubble group was obviously decreased,cell apoptosis increased significantly,Cells invade experiments showed that the number of cells in genetic microbubble group (22.18 ± 2.01) decreased significantly compared with the control group and the nontargeted group,can effectively inhibit the proliferation and invasive ability of HepG2 cells.Conclusion Targeted ultrasound microbubble carrying target gene have better inhibitory effect on HepG2 cells in vitro.

Tecnología de información y adhesión de guías de práctica clínica en el nivel de atención asistencial primario: experiencia privada, 2013-2014 / Information technology and adherence to clinical practice guidelines in primary assistant care level: Private Experience, 2013-2014

Objetivo: Se describió la variación de la adherencia de las Guías de Práctica Clínica (GPC), luego de la implementación del módulo "terapéutica sugerida" en el sistema informático asistencial de un establecimiento de salud privado en La Libertad. Material y métodos: Se comparó el nivel de adherencia trimestral de las GPC antes y después de la intervención, mediante auditoría de la información asistencial. Resultados: El nivel de adherencia de las GPC antes de las modificaciones estuvo entre 63% y 65%, observándose un incremento hasta 75% posterior a la implementación del módulo "terapéutica sugerida". Conclusión: La implementación de módulos facilitadores de la acción asistencial en el sistema informático podría mejorar la adhesión a las GPC, lo que sugiere la necesidad de replicar otros estudios

Objective: The variation of the adhesion of the Guidelines of Clinical Practice (GPC), after the implementation of the "suggested therapeutic" module in the computer system of a private health facility in La Libertad was described. Material and methods: The level of quarterly adherence of GPC was compared before and after the intervention, by an audit of healthcare information. Results: The level of adherence of GPC before the amendments was between 63% and 65%, with an increase to 75% after the implementation of the "suggested therapeutic" module. Conclusion: Use of facilitator's modules of healthcare assistance in the information system could improve adherence to GPC, which suggest the need to replicate other studies

Guía de Práctica Clínica para el Cuidado de Enfermería a la familia del paciente pediátrico en la UCI / Clinical Practice Guideline for Nursing Care for the family of pediatric patients in the ICU

La enfermedad crítica de los niños y su ingreso a la Unidad de Cuidados Intensivos (UCI) afecta de manera negativa a las familias. Enfermería tiene un papel importante frente a las necesidades de estas familias, identificándolas y valorándolas oportunamente para ser intervenidas y satisfechas. Este estudio propone una Guía de Práctica Clínica (GPC) basada en la evidencia científica con recomendaciones fiables, esenciales para el Cuidado de Enfermería a la familia del niño hospitalizado en la UCI. Para su elaboración se identificaron 12 temas de interés, a partir del consenso de expertos, agrupados en 4 categorías con un total de 29 preguntas en formato PICO. Se realizó una búsqueda de literatura en 16 bases de datos, 1 motor de búsqueda y 1 revista indexada, utilizando palabras claves en español e inglés. Se seleccionaron 43 de 279 estudios revisados, mediante el análisis y la aplicación de una lista de chequeo que evaluaba la calidad del estudio dependiendo de la metodología utilizada. Posteriormente se calificó el nivel de evidencia teniendo como referencia el sistema de clasificación de la SIGN. Las piezas investigativas, directamente relacionadas con el tema de interés, dieron respuesta a las preguntas y permitieron establecer 47 recomendaciones. En general, la evidencia científica encontrada corresponde a los niveles 1+, 2++, 2+, 3 y 4 según la clasificación SIGN y las recomendaciones generadas son grado B, C y D.

The critical illness of children and their admission to the Intensive Care Unit (ICU) negatively affects families. Nursing plays an important role in addressing the needs of these families, identifying and assessing them in a timely manner to be intervined and satisfied. This study proposes a Clinical Practice Guideline (CPG) based on the scientific evidence with reliable and essential recommendations for Nursing Care to the family of the child hospitalized in the ICU. For its elaboration, 12 topics of interest were identified, based on the consensus of experts, grouped into 4 categories with a total of 29 questions in PICO format. The literature search was carried out on 16 databases, 1 search engine and 1 indexed journal, using key words in Spanish and English. 43 of 279 studies reviewed were selected, through analysis and application of a checklist that evaluated the quality of the study depending on the methodology used. Subsequently, the level of evidence was rated based on the classification system of the SIGN. The pieces of research, directly related to the topic of interest, answered the questions and allowed 47 recommendations. In general, the evidence corresponds to levels 1+, 2++, 2+, 3 and 4 according to the SIGN classification and the recommendations generated are grade B, C and D.

Determination of the content and the molecular weight of Mussel Polysaccharide by GPC / 中国生化药物杂志

Objective To develop a gel permeation chromatography method for determination of content and molecular weight ( Mw ) of Mussel Polysaccharide.Methods Using GPC method, the sample was separated with TSK-gel GMPWXL(7.8 mm ×300 mm) chromatography column which was set at 35℃.The mobile phase was 0.05 mol/mL NaNO3(including 0.05%Na2N3) and the flow rate was 0.6 mL/min.The detector was RID-20AT. Results The average molecular weight of the polysaccharide of Mytilus coruscus was 1 261 411 and the average content was 88.6%by using of the calibration curves of dextrans.The average molecular weight of the polysaccharide of Mytilus edulis was 1 244 062 and the average content was 87.4%. Conclusion The method established in this paper is simple and rapid, accurate and reproducible, which can be used for the quality control of Mussel Polysaccharide.

Lentivirus vectors construction of SiRNA targeting interference GPC3 gene and its biological effects on liver cancer cell lines Huh-7

OBJECTIVES@#To build GPC3 gene short hairpin interference RNA (shRNA) slow virus vector, observe expression of Huh-7 GPC3 gene in human liver cell line proliferation apoptosis and the effect of GPC3 gene influencing on liver cancer cell growth, and provide theoretical basis for gene therapy of liver cancer.@*METHODS@#Hepatocellular carcinoma cell line Huh-7 was transfected by a RNA interference technique. GPC3 gene expression in a variety of liver cancer cell lines was detected by fluorescence quantitative PCR. Targeted GPC3 gene sequences of small interfering RNA (siRNA) PGC-shRNA-GPC3 were restructured. Stable expression cell lines of siRNA were screened and established with the help of liposomes (lipofectamine(TM2000)) as carrier transfection of human liver cell lines. In order to validate siRNA interference efficiency, GPC3 siRNA mRNA expression was detected after transfection by using RT-PCR and Western blot. The absorbance value of the cells of blank group, untransfection group and transfection group, the cell cycle and cell apoptosis were calculated, and effects of GPC3 gene on Huh-7 cell proliferation and apoptosis were observed.@*RESULTS@#In the liver cancer cell lines Huh-7, GPC3 gene showed high expression. PGC-shRNA-GPC3 recombinant plasmid was constructed successfully via sequencing validation. Stable recombinant plasmid transfected into liver cancer cell lines Huh-7 can obviously inhibit GPC3 mRNA expression level.@*CONCLUSIONS@#The targeted GPC3 siRNA can effectively inhibit the expression of GPC3.

Preparation and Preliminary Application of Monoclonal Antibody Against Carcinoembryonic Antigen Glypican-3 / 天津医药

Objective To prepare monoclonal antibody specifically against carcinoembryonic antigen glypican-3 (GPC3) and its preliminary application. Methods GPC3 was cloned with PCR to pET16b vector and expressed in E. co-liBL21. Spleen cells were obtained from Balb/c mice embedding immunized with purified antigen intraperitoneally, and fused with Sp2/0 cells. Hybridoma cells were screened by indirect ELISA, and identified by Western blot assay using puri-fied protein after the cell fusion. The indirect immunofluorescence method was used to detect the GPC3 expression in HepG2 cell line. Results The prokaryotic expression vector of GPC3 was successfully constructed, and GPC3 was stably expressed in E. coliBL21. A mouse hybridoma cell line secreting monoclonal antibody to GPC3 was obtained. Western blot analysis showed that monoclonal antibody specifically recognized recombinant protein. Monoclonal antibody could be used to detect GPC3 protein expression in HepG2 cell line by indirect immunofluorescence. Conclusion The monoclonal antibody against GPC3 was successfully obtained.

The second international collaborative study on molecular weight of heparin sodium reference standard / 中国药学杂志

OBJECTIVE: To apply the heparin sodium molecular weight reference standard calibrated in the first study period to GPC method and assesse the GPC method.

The first international collaborative study on molecular weight of heparin sodium reference standard / 中国药学杂志

OBJECTIVE: To calibrate the molecular weight of reference standard of heparin sodium.

Mir-520c-3p targeted GPC3 for hepatocellular carcinoma Huh-7cell proliferation, migration and invasion ability / 国际外科学杂志

Objective To research the effect of mir-520c-3p targeted GPC3 to the hepatocellular carcinoma Huh-7 cell proliferation,migration,and the influence of the attack ability and find new theoretical basis for liver hepatocellular carcinoma clinical treatment.Methods The cells were divided into three groups:not transfection of mir-520c-3p group (cell group),negative control group (Nc group),and transfection of mir-520c-3p group (treat ment group).Then used fluorescence quantitative PCR and Western Blot to detect GPC3mRNA gene and protein expression quantity.Cell proliferation of change was detected by the EDU.Made use of Transwell to detect cell invasion and migration ability of the change.Results Fluorescence quantitative PCR results showed that Cell group,NC group and treatment group were 1.13 ± 0.23,1.28 ± 0.15 and 1.05 ± 0.19 (P ＞ 0.05),mir-520-3p could not reduce the GPC3mRNA; but Western Blot detection results showed that GPC3 protein expression level reduce significantly after transfection mir-520c-3p,Cell,NC and treatment group were 2.16 ± 0.08,1.99 ± 0.04 and 0.499 ± 0.05 (P ＜ 0.01).The EDU detection results showed that hepatocellular carcinoma Huh-7 cell proliferation ability obviously inhibited after transfection mir-520c-3p,Cell group,NC group and treatment group were (90.12 ± 1.93) ％,(91.02 ± 0.35) ％ and (77.73 ± 5.88) ％ (P ＜ 0.05),and Transwell test found that hepatocellular carcinoma Huh-7cell invasion abilities were restrained,Cell group,NC group and treatment group were 0.071 ±0.008,0.105 ±0.001 and 0.048 ± 0.002 (P ＜ 0.05),in the same the cells' migration abilities were reduced,Cell group,NC group and treatment group were 0.546 ± 0.010,0.328 ± 0.002 and 0.151 ± 0.002 (P ＜0.01).Conclusions Mir-520c-3p can target GPC3 so that affect hepatocellular carcinoma Huh-7 cell proliferation,invasion and migration abilities.

Construction of GPC3 green fluorescent protein eukaryotic expression vector and its influence on growth promoting effect of growth factors in SK-Hep-1 cells / 第二军医大学学报

Objective: To construct a GPC3 green fluorescent protein eukaryotic expression vector pEGFP-N2-G-PC3, and analyze its effects on the growth promoting effect of growth factors (fibroblast growth factor-2, FGF2; insulin-like growth factor-2, IGF2; transforming growth factor-β1, TGF-β1; and bone morphogenetic protein-4,BMP4) in human hepatoma cell line GPC3-SK-Hep-1. Methods: A eukaryotic expression vector for GPC3 genes (pEGFP-N2-GPC3) was constructed by recombinant DNA technique and was transfected into SK-Hep-1 cells by Lipofectamine™ 2000; the cells stably expressing GPC3 were screened out by G418 (600 μg/ml). The mRNA expression of GPC3 was detected by RT-PCR method and the protein expression of GPC3 by Western blotting and fluorescence microscope. Effects of GPC3 gene on the growth promoting effects of the above growth factors were examined by MTT. Results: The recombinant plasmid was verified to be correctly constructed by restriction endonuclease analysis and DNA sequencing. The green fluorescence was detected in the transfected SK-Hep-1 cells under fluorescence microscope. RT-PCR and Western blotting both confirmed that GPC3 was successfully expressed in SK-Hep-1 cells. FGF2-induced cell proliferation was significantly decreased by GPC3 gene, whereas the growth promoting effects of IGF2, TGF-β1 and BMP4 were not altered by GPC3 gene. Conclusion: We have successfully obtained the synthetic GPC3 protein, which has the same amino acid sequence as that of human GPC3 protein. The eukaryotic expression vector pEGFP-N2-GPC3 has been correctly constructed and GPC3 protein has been successfully expressed in SK-Hep-1 cells. GPC3 may negatively modulate FGF2 signaling pathway.

The Effect of Grape Procyanidins on Hemorheology in Long Distance Runner in vitro and in vivo / 中国慢性病预防与控制

Objective To study the effects of Grape Procyanidins(GPC)on hemorheology in long distance runner.Method In vitro study: 22 long distance runners were divided into two groups randomly,the experimental group and control group.With ten days period,the experimental group was given GPC 200 mg per day;while the control group were given the capsule of starch 200 mg per day.In vivo study: the vein blood samples were taken from 5 long distance runners and every example was divided into five parts,and then treated with different concentrations of GPC or H2O2.Items of the hemorheology such as Er,HCT,Eb,Ep,PFC and VAI were tested both in vivo and vitro before and after the study.Results in vitro study: all items of the experimental group showed significant decline at then end of the study than those before the study.The contents of Er,Eb and Ep before the study(6.830?0.164,4.145?0.177,1.647?0.020,respectively) were significantly higher than those after the study(6.759?0.158,4.088?0.173,1.621?0.013,respectively)(P

Influence of glypican-3 gene on proliferation,adhesion and invasion of hepatoma carcinoma cancer cell line SK-Hep-1 / 中国肿瘤生物治疗杂志

Objective:To study the influence of GPC3 gene on the proliferation,adhesion and invasion of hepatoma cell line SK-Hep-1.Methods:SK-Hep-1 cells were transfected with pEGFP-N2-GPC3 using Lipofectamine2000.RT-PCR was used to examine the GPC3mRNA expression in GPC3-SK-Hep-1 cells.MTT assay was used to examine the proliferation and calculate the adhesion rate of SK-Hep-1 cells.Transwell system was used to assess the migration and invasion of the cells.Results:SK-Hep-1 cells were successfully transfected with pEGFP-N2-GPC palsmid.GPC3 mRNA was detected in SK-Hep-1 cells.Transfection with CPC3 significantly suppressed the growth of SK-Hep-1 cells(P