Cloning of p15~( INK4b) related gene CCRG and expressio n analysis in the renal carcinoma / 中国病理生理杂志

AIM and METHODS: To analysis the factor that involved in renal carcin ogenesis, we used the bait gene AK001518 to screen GenBank. To understand the re lationship between cell cycle related gene(CCRG) and p15, we did RT-PCR and Nort hern Blot experiments. Th en we examined CCRG expression level in renal carcinogenesis. RESULTS: Gained a function unknown gene CCRG that was 67% a mino acid identical with the gene AK00 1 518 that was regulated by p15. It was shown that the CCRG mRNA was dramatically d ecreased when p15 gene was over-expressed. CCRG expression level was much higher i n tumor tissues and cells than normal tissues and cells. CONCLUSION: The novel g ene CCRG expressed highly in the renal carcinoma, which might play a significant role in the renal carcinogenesis.

Methylation of p15INK4B gene and its mechanism in patients with myelodysplastic syndrome and leukemia / 中国病理生理杂志

AIM: To explore the relationship between hypermethylation of p15INK4B gene and the pathogenesis of hematopoietic malignances. METHODS: The expression and methylation of p15INK4B gene and the expression of DNA methyltransferase genes (DNMTs) in bone marrow cells from 54 cases with hematopoietic malignances were detected by RT-PCR, Western blot, and methylation-specific PCR. RESULTS: The p15INK4B gene was methylated more often in high-risk myelodysplastic syndrome (MDS) patients, patients at blast phase of chronic myeloid leukemia (CML-BP) and acute leukemia patients than that in low-risk MDS patients (P

Cloning and expression of Treponema pallidum specific antigens P15 and P47 for clinical application / 第二军医大学学报

Objective: To clone and express Treponema pallidum (TP) specific antigens P15 and P47 and use them in clinical examination. Methods: P15 and p47 genes synthesized were inserted into a GST fusion expression vector. The recombinant antigens were purified by affinity chromatography and then coated on microplates to establish an ELISA kit. Using this kit, national TP standards, sera of normal persons and patients with TP and other diseases were tested. Results: The synthesized p15 and p47 genes sequenced were identical with those of GenBank. National TP standards were tested by ELISA kit which coated with these recombinant antigens, and the results accorded with the standards. High sensitivity and specificity was showed when 2 recombinant antigens were used in ELISA to detect the sera of normal persons and patients with TP and other diseases. Conclusion: The recombinant TP specific antigen P15 and P47 are suitable for establishment of ELISA kit in clinical examination.