RESUMO
@#The information of biomechanical properties is crucial in the study of biological tissue and its clinical relevance. 3mm x 3mm free gingival human tissues was taken using disposable punch biopsy (Accu sharp blade, India) and stored in 0°C Freezer. The sample was sectioned to a thickness of 10μm using high profile microtome blade (Leica 818, Germany) and cryostat (Leica CM1850UV, United Kingdom). The sample was analysed using Atomic Force Microscope (Nanowizard® 3, JPK Instruments, Germany) at room atmosphere. The collagen fibrils of the free gingival tissues appeared to be stacked in basket weave like structure. The mean value of free gingival collagen fibrils width and the length of D-band were 106.71±11.18nm and 65.82 ± 3.04nm respectively. The Young’s modulus of collagen fibrils for human free gingival tissue at overlap region was 212.88 ± 242.58 MPa, whereas at the gap region was 207.00 ± 230.71 MPa. Within the limitation of the study, the collagen fibrils appeared to be stacked in basket weave-like structure. The length and width of the collagen fibril were similar to the values investigated using other techniques. There was significant linear relationship between Young’s modulus of overlap and gap regions.
RESUMO
Despite high cure rates, approximately 20% of patients with acute lymphoblastic leukemia (ALL) have disease relapse. Isolated recurrence in oral cavity is extremely unusual. The aim of this paper is to report a case of an isolated relapse occurred in a child with T-lineage ALL. Clinical picture included swelling and pain in the right upper gingiva of the oral cavity, with no other clinical or hematological alterations. Diagnosis was confirmed by biopsy and immunohistochemical staining. Bone marrow aspiration was normal. Five months later leukemic infiltration of the bone marrow was detected and systemic chemotherapy was reintroduced. This case report highlights the relevance of dental care during and after chemotherapy, not only to treat lesions in the oral cavity resulting from the disease itself or from treatment side effects, but also to detect unusual sites of ALL relapse.
Apesar dos altos índices de cura, cerca de 20% dos pacientes com leucemia linfóide aguda (LLA) apresentam recidiva da doença. Recidiva isolada na cavidade oral é extremamente incomum. O objetivo deste trabalho é relatar um caso de recidiva isolada em criança com LLA de linhagem T. A apresentação clínica foi quadro de edema e dor na cavidade oral, na região superior da gengiva à direita, sem outras alterações clínicas ou hematológicas. O diagnóstico foi confirmado por meio de biópsia e imuno-histoquímica. O mielograma era normal. Cinco meses após a manifestação inicial na cavidade oral, foi detectada infiltração leucêmica na medula óssea. O tratamento com quimioterapia sistêmica foi reintroduzido. Este relato de caso ressalta a importância do acompanhamento clínico e odontológico durante e após o tratamento quimioterápico, não somente com o objetivo de tratar as alterações na cavidade oral decorrentes da própria doença ou dos efeitos adversos do tratamento, mas para que sejam detectadas apresentações incomuns de recidiva na LLA.