Buprenorphine fentanyl transdermal system combined gone.love Buna needle in artificial knee joint replacement long-acting analgesic effect / 中国生化药物杂志

Objective To study the transdermal buprenorphine Finland combined needle in patients in Buner after total knee arthroplasty long-acting analgesic effect. Methods 60 cases of total knee arthroplasty in Zhoushan Guang'an Hospital from February 2015 to August 2016 were selected as the study subjects, and were randomly divided into the control group and the experimental group, each group had 30 cases. The postoperative patients in control group were given Bouna needle, given buprenorphine finland transdermal patch on the basis of the experimental group. If the visual analogue scale of the two patients was greater than 5, tramadol hydrochloride sustained release tablets was given. The analgesic effects of the experimental group and the control group were compared and analyzed. Results After seventh days of treatment, the resting pain score of the experimental group was (1.11±0.43), significantly lower than that of the control group (2.50±0.68), with statistical significance (P<0.05). Seventh days after treatment, the daily dose of tramadol in the experimental group was (210.09±20.90) mg, significantly less than that of the control group (1123.90±60.90) mg, with statistical significance (P<0.05). There were no obvious adverse reactions in the two groups, and there was no significant difference. Conclusion The application of buprenorphine transdermal patches combined with finland gone.love Buna needle in artificial knee joint replacement surgery, the analgesic effect is obvious, can to some extent reduce the dosage of analgesic, side effects may still need to observe.

Buprenorphine fentanyl transdermal system combined gone.love Buna needle in artificial knee joint replacement long-acting analgesic effect / 中国生化药物杂志

Objective To study the transdermal buprenorphine Finland combined needle in patients in Buner after total knee arthroplasty long-acting analgesic effect. Methods 60 cases of total knee arthroplasty in Zhoushan Guang'an Hospital from February 2015 to August 2016 were selected as the study subjects, and were randomly divided into the control group and the experimental group, each group had 30 cases. The postoperative patients in control group were given Bouna needle, given buprenorphine finland transdermal patch on the basis of the experimental group. If the visual analogue scale of the two patients was greater than 5, tramadol hydrochloride sustained release tablets was given. The analgesic effects of the experimental group and the control group were compared and analyzed. Results After seventh days of treatment, the resting pain score of the experimental group was (1.11±0.43), significantly lower than that of the control group (2.50±0.68), with statistical significance (P<0.05). Seventh days after treatment, the daily dose of tramadol in the experimental group was (210.09±20.90) mg, significantly less than that of the control group (1123.90±60.90) mg, with statistical significance (P<0.05). There were no obvious adverse reactions in the two groups, and there was no significant difference. Conclusion The application of buprenorphine transdermal patches combined with finland gone.love Buna needle in artificial knee joint replacement surgery, the analgesic effect is obvious, can to some extent reduce the dosage of analgesic, side effects may still need to observe.

Osteoblast differentiation and mature gene regulation inside femoral head with steroid-induced osteonecrosis in response to mechanical stress stimulation / 中华创伤杂志

Objective To study dynamic changes of gene expressions and protein synthesis of Runx2 (runt-related transcription factor-2), Osterix, osteocalcin and AJ18 inside the femoral head with steroid-induced osteonecrosis after mechanical stress stimulation in rats. Methods A total of 50 Wistar rats (half male in sex) weighing 250-270 g (mean 260 g) were involved in this study and randomly divided into experimental group (40 rats) and normal group (10 rats). The rats in experimental group were injected with dexamethasone (20 mg/kg) via bilateral gluteus maximus alternatively once a week and then trained on laboratory animal treadmill twice weekly to make rat model of femoral head necrosis. After identifying the successfully induced model by Hematoxylin and eosin stain, glucocorticoid injection was ceased and the experimental group was randomly divided into model control group, 4 weeks, 6 weeks, 8 weeks groups after hormone training stopped. Then, total RNA and total protein were extracted from femoral head for detecting dynamic changes of genes expressions and proteins synthesis of Runx2, Osterix, osteocalcin and A J18 after mechanical stress stimulation inside the femoral head with steroid-induced osteonecrosis by means of real-time quantitative PCR and Western blot assay. Results In 4 weeks, 6 weeks, 8 weeks groups after hormone training stopped, the gene expressions and proteins synthesis of Runx2, Osterix and osteocalein were reduced more significantly compared with model control group, mBNA expression values of Runx2, Osterix and osteoealcin were 0. 1809, 0. 1639, 0. 1374 and 0. 4219, 0. 3026, 0.2652 and 0. 2857, 0.2027, 0. 1583 times of those in model control group. The expressions of Runx2, Osterix and osteocalcin showed a downward trend with time. The mBNA expression and protein synthesis of AJ18 at 4th, 6th and 8th weeks after hormone training stopped were 2.6391,4. 2718 and 5. 3165 times of model control group. Conclusions In addition to hormonal factors, inappropriate mechanical stress inhibits expressions and proteins synthesis of Runx2, Osterix and osteocalcin, while the expression and protein synthesis of AJ18 are upgraded in early steroid-induced femoral head necrosis in rats.

Construction of recombinant adenovirus vector with HSV-TK controlled by hTERT promoter and experimental study of anti-hepatoma / 肿瘤研究与临床

Objective To construct the recombinant adenovirus vector with hTERT-HSV-TK and observe the killing effect of Ad-hTERTp-HSV-TK/GCV system on hepatocellular carcinoma cells. Methods A recombinant replication defective adenoviral vector of Ad-hTERTp-HSV-TK was constructed via homologous recombination which both shuttle plasmid pSU-Tp-TK and adenovirus backbone plasmid pBHGE3 transfected into the HEK293 packaging cells. Then the Ad-hTERTp-HSV-TK was amplified and purified through PCR. The activity of the HepG2 cells and the L-02 cells were tested by methyl thiazolyl terazolium (MTT) after they were transfected by the recombinant adenovirus of different multiplicities of infection (MOI) and then were added GCV of different conc.entration. Results The recombinant replication defective adenoviral vector of Ad-hTERTp-HSV-TK were identified by PCR successfully. The viral titer was 1.5×1010 pfu/ml after amplification and purification. The HepG2 cells were targetedly suppressed by Ad-hTERTp-HSV-TK/GCV system. The survival rate of cells decreased gradually along with the increase of the MOI and the GCV' s concentration. Conclusion The recombinant replication defective adenoviral vector of Ad-hTERTp-HSV-TK can inhibit the HepG2 cells significantly, but has not influence on the L-02 cells.

Construction of Adeno-associated Virus System for Human Bone Morphogenetic Protein 7 Gene / 华中科技大学学报（医学）（英德文版）

To construct the recombinant adeno-associated virus (rAAV) vector with human bone morphogenetic protein 7 (BMP7) and observe the BMP7 mRNA expression in vitro, BMP7 CDS se- quence was cloned into expression plasmid pAAV-MCS of AAV Helper Free System. The recombi- nant plasmid was identified with enzyme digestion and sequencing. The recombinant plasmid, pAAV-RC, pHelper were co-transfected into AAV-293 cells according to the calcium phosphate-based protocol. The viral stock was collected by 4 rounds of freeze/thaw. After purified and concentrated,the recombinant virus titer was determined by dot-blot assay. HEK293 cells were transfected with the recombinant virus at different MOI, and the expression of BMP7 mRNA was detected by RT-PCR. The results showed rAAV-BMP7 was constructed and packaged successfully. The physical particle titer was 2.5×1011 vector genomes/mL. There was different expression level of BMP7 mRNA after transfecton. These data suggested that recombinant AAV mediated a stable expression of hBMP7 mRNA in 293 cells. The AAV production method may pave the way of an effective strategy for the jaw bone defection around dental implants.

Relationship between the Expression of RASSF1A Protein and Promoter Hypermethylation of RASSF1A Gene in Bladder Tumor / 华中科技大学学报（医学）（英德文版）

To investigate the relationship between the expression of RASSFIA protein and promoter hypermethylation of RASSFIA gene, RASSFIA protein expression was measured by Western blot- ting in 10 specimens of normal bladder tissues and 23 specimens of bladder transitional cell carci- noma (BTCC). The promoter methylation in BTCC and normal bladder tissues was detected by me- thylation-specific PCR (MSP). The results showed that the expression level of RASSFIA protein was significantly lower in BTCC tissues than that in normal bladder tissues. However, it was not corre- lated with its clinical stages and pathological grades. The frequency of promoter methylation of RASSF1A gene was higher in BTCC tissues than that in normal bladder tissues. In 14 patients with the aberrant promoter methylation, 13 showed loss or low expression of RASSF1A protein. It is con- cluded that RASSFIA gene promoter methylation may contribute to the low level or loss of RASSFIA protein expression, the inactivation of RASSFIA gene and the genesis of BTCC. But, it may bear no correlation with its clinical stages and pathological grades.

Preliminary study on serum levels of IL-2, IL-6 and TNF-α and their gene polymorphism in copper miners / 中华放射医学与防护杂志

Objective To study biologic effect of high radon exposure in non-uranium miners by measurements of serum levels of 1L-2, IL-6 and TNF-u and study on their gene polymorphism. Methods Serum levels of IL-2, IL-6 and TNF-αin the miners selected from a Yunnan-based copper mine were measured by ABC-ELISA. TNF-α (-308,G→A) genotypes were identified by RFLP-PCR, IL-2 (-330, T→G) genotypes by sequence analysis. Results Compared the miners with its control group, there were no statistical significance of the concentrations of serum IL-2 (F=0.71, P>0.05), IL-6 (F=1.09, P>0.05) and TNF-α(F=0.95,P>0.05). Frequencies of IL-2 (-330, T→G) genotypes (X2=0.02, P>0.05) and TNF-α(-308, G→A) genotype (x2= 2.21, P>0.05) were shown no statistical significance too. Conclusions Compared with the control group, concentrations of serum IL-2, IL-6 and TNF-n in miners working in the copper mine was lower, frequencies of genotypes of TNF-o (-308,A/A) was higher in the miners. But all the differences were not statistical significant.