Advances in studies on naphthoquinones from green walnut husks of Juglans mandshurica and their anticancer activities / 中草药

Green walnut husks of Juglans mandshurica is the exocarp of the immature fruit of Juglans mandshurica. It has been used for thousands of years as a traditional Chinese herbal medicine. It is mainly used for antipyretic and detoxification, antibacterial and anti-inflammatory, analgesic and anti-malarial, and so on. In recent years, its anticancer activity has attracted widespread attention from domestic and foreign scientists. Modern pharmacological studies have shown that naphthoquinones are the main anticancer active ingredients of green walnut husks of J. mandshurica. In this review, we combed the naphthoquinone chemical components extracted from green walnut husks of J. mandshurica and described the chemical constituents of naphthoquinones with strong anticancer activity. It provides an important scientific basis for clarifying the pharmacodynamic basis of the anti-cancer effect of Chinese medicine green walnut husks of J. mandshurica.

Chemical constituents of n-butanol fraction from green walnut husks of Juglans mandshurica / 中草药

Objective To study the chemical constituents of the n-butanol part which extracted from green walnut husks of Juglans mandshurica with antitumor activity. Methods The chemical constituents from 95% EtOH extract of green walnut husks of J. mandshurica at room temperature were isolated and prepared by silica gel column, reversed-phase ODS, and pre-HPLC after n-butanol extraction, and their structures were elucidated by a variety of spectral and spectroscopic techniques. Results Eight compounds were isolated and identified as 4-hydroxy-4-(3’-hydroxyphenol) butanoic acid-4-O-β-D-glucopyranoside ethyl ester (1), 4-hydroxy- 4-(3’-hydroxyphenyl) butyric acid-4-O-β-D-glucopyranoside methyl ester (2), 1,4,8-trihydroxynaphthalene-1-O-β-D-glucopyranoside (3), 1,4,8-trihydroxy-3-naphthoic acid ethyl ester-1-O-β-D-glucopyranoside (4), (4S)-4,5,8-trihydroxy-α-tetralone-4-O-β-D- glucopyranoside (5), (4S)-4,5,8-trihydroxy-α-tetralone-5-O-β-D-[6’-O-(3″,4″,5″-trihydroxybenzoyl)] glucopyranoside (6), (4S)-4- hydroxy-α-tetralone-4-O-β-D-(6’-O-4’’-hydroxylbenzoyl)-glucopyranoside (7), and (4S)-4,5-dihydroxy-α-tetralone-4-O-β-D-(6’-O- 4’’-hydroxylbenzoyl)-glucopyranoside (8). Conclusion Compound 1 named juglanoside P and compound 2 namded juglanoside Q are both new compounds.