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Objective To explore the physical/chemical properties and effect of photodynamic therapy (PDT) of iminodiacetic acid modified tetraphenylporphyrin on mice bearing H22 liver cancer.Methods The UV absorbance spectrum,singlet oxygen yield and lipid-water partition coefficient were measured by UV spectrophotometry.Mice bearing H22 liver cancer as animal model were divided into control group (no drug,no light),light treated group (no drug,only light),drug treated group (only drug,no light),experimental once group (with drug and light,once) and experimental twice group (with drug and light,twice,PDT twice).Tumor inhibition rate and index of liver,spleen,lung,thymus of the mice were calculated to evaluate the antitumor activity of iminodiacetic acid modified tetraphenyl porphyrin-PDT.Results The iminodiacetic acid modified tetraphenylporphyrin had absorption at 650 nm,while its singlet oxygen yield and lipid-water partition coefficient were 2.30 and 0.52,respectively.No antitumor effect on mice bearing H22liver cancer for light treated group and drug treated group respectively.However,significant antitumor effect (P＜0.001)was found at experimental group.The maximum tumor inhibition rate could be 95.15％ for PDT twice group.No significant differences were observed on weight gain and liver,lung,spleen,thymus index in each group.Conclusion Iminodiacetic acid modified tetraphenylporphyrin with high singlet oxygen yield,low toxicity and significant in vivo anti-tumor activity,is suitable to be developed as an anti-tumor drug candidate for photodynamic therapy.
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[Objective]To study the inhibitory effect of the extract of Rhizoma Amorphophallus,we set up the Kunming mices which were vaccinated with H22 tumor as experimental model,and explore the possibility mechanism of them.[Method]We adopted the mices vaccinated with H22 tumor as experimental model to observe the influence of different extracts on the antitumor ratio,and the cooperating antitumor effect together with 5-Fu.Using flow cytometry to detect the effect of petroleum ether extract on apoptotic cells.Using immunohistochemical method to observe the influence of this extract to the expression of Survivin and Bax(Bcl-2 associated protein x).[Result]The extract of petroleum ether of Rhizoma Amorphophalli had inhibitory effect of the growth on mice vaccinated with H22 tumor,and the possible mechanism was the downregulation of Survivin expression and upregulation of Bax expression to induce apoptosis of tumor cell.
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Objective To observe the anti-tumor effect of ethaselen on transplanted liver cancer(H22)and Lewis lung cancer mice.Methods The transplanted liver cancer(H22)and Lewis lung cancer mice models were established.Each genus consisted of 60 mice,which were divided into control,CTX,and three ethaselen dose groups,respectively,with 12 mice in each.Intraperitoneal injection(ip.)of three dosages was performed once a day through the abdominal wall separately,from the second to the eighth day after cancer was transplanted.On the eleventh day,six mice in each group were killed to calculate the tumor inhibition ratio and observe the cell cycle by flow cytometry,and the others were observed for the life extension ratio.Results When ethaselen was given at the dosage of 25,12.5 and 6.3 mg/kg,the tumor inhibition ratio was 52.5%,43.6% and 30.2%,and the life extension ratio was 56.6%,38.7% and 14.2% in the transplanted liver cancer(H22)mice,respectively.The tumor inhibition ratio was 43.8%,29.6% and 18.9%,and the life extension ratio was 47.3%,17.9% and 6.3% in the transplanted Lewis lung cancer mice,respectively.Compared with that of the control,the apoptosis ratio was obviously increased in each group,and there was obviously induced S phase arrest in 25.0 mg/kg(P