Development Of Bioanalytical Standardization Parameters Of Alocasia Indica Tuber By Hptlc Technique

Alocasia indica is perennial herb growing widely and used as traditional medicine in India, China and Bangladesh. The divine herb has potent medicinal values for the treatment of different type of illnesses. The HPTLC techniques were used to separate active components from ethanolic extract of tuber part of A. indica. This examination was intended to designed a HPTLC fingerprint profile of crude extract of the plant in ethanol. A HPTLC method for the isolation of various active constituents in A. indica ethanolic extract have been developed and solvent system for quercetin the mobile phase used was toluene: ethyl acetate: formic acid (5:2:1) and for analysis of β-sitosterol the mobile phase used was chloroform: ethyl acetate: formic acid (6:4:1) . In the present investigation, HPTLC fingerprint of extract of dried tuber part of A. indica have been performed and the results demonstrated that important information for standardization. The HPTLC system for routine quality control of present species can be used for ethanolic extract and serve in qualitative, quantitative and was appropriate for standardization of the plant.

Pharmacognostic characterization of Myrica esculenta leaves

Background: Myrica esculenta (Family: Myricaceae) commonly known as Kaiphala or Katphala is a widelyused medicinal plant in Ayurveda. In spite of its numerous medicinal attributes, no published work isavailable till date on pharmacognostical characterization and HPTLC analysis of its leaves.Objectives: To investigate the pharmacognostical, physicochemical, and HPTLC profiles of M. esculentaleaves.Materials and methods: The measures taken for pharmacognostical characterization were organolepticstudy, macroscopy, microscopy, powder microscopy, leaf constant, fluorescence analysis, preliminaryphytochemical screening and HPTLC spectra profile.Results: Organoleptic and macroscopic studies found that leaves are lancoelate, thin, spirally arranged,dark green in color, with an astringent taste and acute apex. In transverse section, cuticularisedepidermis having polygonal cells were found. Mesophyll cells were differentiated into single layeredpalisade cells on each surface and 2e3 layered spongy parenchyma, unicellular and uniseriate hollowtrichomes, anomocytic stomata and bowl shaped vascular bundle in mid rib portion containing xylemand phloem tissues. Alkaloids, carbohydrates, flavonoids, glycosides, phenolic compounds and tanninswere found present. Analysis on the leaf constants, powder microscopy, fluorescence characteristics andphysical parameters resulted a valuable data to establish standards for the plant. HPTLC profile providesnumber of constituents present in the extracts with their respective Retention Factor (Rf).Conclusion: Present report on pharmacognostical characterization and HPTLC analysis of M. esculentaleaves provides a vital diagnostic tool for identification, authentication and development of quality parameters of the species. Data obtained by present study may be considered as standard for future studies.© 2017 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Publishing Services byElsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

HPTLC finger print analysis of phytocompounds and in vitro antioxidant activity of Eugenia singampattiana Bedd.

This paper has reported the preliminary phytochemical screening, HPTLC analysis of phytocompounds and in vitro antioxidant activities of ethanol extract of Eugenia singampattiana leaves. This is the first report on the antioxidant activity of this plant. The preliminary phytochemical analysis showed the presence of alkaloids, coumarin, catechin, steroids, flavonoids, saponins, phenols, glycosides and terpenoids. HPTLC analysis also confirmed the presence of alkaloids, steroids, flavonoids, saponins, phenols, glycosides and terpenoids. The antioxidant activities of the leaves in ethanol extract are assessed using different models like DPPH, superoxide radical, hydroxyl radical and ABTS+ cation radical and reducing power at different concentrations. The ethanol extract at 800μg/ml showed maximum scavenging activity. Results obtained revealed that, ethanol extract of leaves of E. singampattiana possess high antioxidant activity. Thus this study suggests that, E. singampattiana plant can be used as a potent source of natural antioxidant.

Biochemical study of caralluma species to understand species homology.

Demarcation of species is the prime endeavor of Biosystematics. conventional morphological parameters and the use of biochemical components in solving taxonomic disputes are gaining importance with the help bioactive phenolic compound as a genetic marker to assess species affinity. In present study methanolic extracts of six different Caralluma species were carried out through phytochemical screening and HPLTC analysis to ascertain relative relationship between active compounds., High Performance TLC on silica gel 60 F254 TLC aluminum sheet was used to separate the active compounds present in the extracts and scanned at 254 nm. The results revealed the presence of alkaloids, flavonoids, saponins and steroids. A partial biochemical profile for selected constituents of species was compared with that of other species in Caralluma by HPTLC to ascertain their relative phylogenetic position. From this phytochemical analysis, the paired affinity, group affinity and isolation value of each of these pair of species of Caralluma are expressed in percentage to study the species homology.