Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders / 神经科学通报·英文版

Neuroplastin 65 (Np65) is an immunoglobulin superfamily cell adhesion molecule involved in synaptic formation and plasticity. Our recent study showed that Np65-knockout (KO) mice exhibit abnormal cognition and emotional disorders. However, the underlying mechanisms remain unclear. In this study, we found 588 differentially-expressed genes in Np65-KO mice by microarray analysis. RT-PCR analysis also revealed the altered expression of genes associated with development and synaptic structure, such as Cdh1, Htr3a, and Kcnj9. In addition, the expression of Wnt-3, a Wnt protein involved in development, was decreased in Np65-KO mice as evidenced by western blotting. Surprisingly, MRI and DAPI staining showed a significant reduction in the lateral ventricular volume of Np65-KO mice. Together, these findings suggest that ablation of Np65 influences gene expression, which may contribute to abnormal brain development. These results provide clues to the mechanisms underlying the altered brain functions of Np65-deficient mice.

Association between diarrhea-predominant irritable bowel syndrome and HTR3A, HTR3E gene polymorphism in Yangzhou, Jiangsu province, China / 中华流行病学杂志

Objective To investigate the relationship between diarrhea-predominant irritable bowel syndrome (D-IBS) and HTR3A,HTR3E gene polymorphism in Yangzhou,Jiangsu province.Methods Polymerase chain reaction (PCR) amplification and restriction fragment length pdymorphism (RFLP) technique were used to detect the 5′-UTR variant c.-42C＞T of HTR3A and 3′-UTR variant c.* 76G＞A of HTR3E on 300 healthy subjects and 450 patients with D-IBS.Results There was significant difference noticed between the D-IBS patients and the controls in the genotype of c.-42C＞T sites (P＜0.05),while the frequency of T allele was significantly higher in both female and male patients than that in the controls (P＜0.05).The frequencies of G/A genotype and A allele of c.*76G＞A sites were significantly higher in the female-patient group than that in the controls (P＜0.05),however,there was no significant difference between those male patients and controls (P＞0.05).When comparing to the CC genetype of rs1062613 site and GG genetype of rs62625044 site,the TT and GA genotype were closely related to the D-IBS,with the risk coefficients as 0.29 (95％ CI:0.14-0.61) and 0.62 (95％ CI:0.39-0.99),with statistically significant differences.Conclusion T allele and GA genetype might respectively serve as the predisposing factors of D-IBS and on the female D-IBS patients.