Analysis of results of genotyping and screening for infertile subjects with alpha-thalassemia in Guangxi / 实用医学杂志

Objective Investigation of the carrier rate, genotyping and genotype frequency of alpha-thalassemia among infertile subjects in Guangxi, and analysis of the relationship between the results of hemoglobin electrophoresis and hematologic parameters among patients with three phenotypes of alpha-thalassemia and subjects with non-thalassemia. Methods The preliminarily patients who were diagnosed as beta-thalassemia via HbA2>3.5％ and/or HbF> 2％ were excluded. Alpha-thalassemia genes of 10 020 infertile subjects were detected in our center from 2017 to 2018, and the results of hemoglobin electrophoresis and hematologic parameters in patients with three phenotypes of alpha-thalassemia were compared. Results 624 patients with alpha-thalassemia were confirmed via gene diagnostic technique, including 19 genotypes, total carrier rate for 6.23％, 275 (2.74％) patients with silent alpha-thalassemia, 326 (3.25％) patients with alpha-thalassemia trait and 23 (0.23％) patients with HbH disease. The most common genotype was--SEA/αα, followed by-α3.7/αα and α, CSα/αα. The parameters of MCV, MCH, MCHC, Hb, HCT, HbA2 were lower, but the value of RBC were higher (both P < 0.05) , in patients with three phenotypes of alpha-thalassemia than subjects with non-thalassemia. The parameters mentioned above excluding HbA2 in patients with alpha-thalassemia showed the following regularity : silent alpha-thalassemia> potential alpha-thalassemia> HbH disease. RBC value tended to increase gradually in patients with silent alpha-thalassemia, potential alpha-thalassemia and HbH disease. Conclusion Infertility with alpha-thalassemia is very popular in Guangxi, especially--SEA/αα which is the most common genotype. The value of MCV, MCH, MCHC, Hb, RBC and HCT are conducive to screening for infertile subjects with alpha-thalassemia, and have certain clinical value for differentiating three phenotypes of alpha-thalassemia.

Suplementação com imunoestimulante em cadelas com neoplasia mamária maligna: aspectos hematológicos e bioquímicos / Immunostimulant supplementation for bitches with malignant mammary tumor: hematological and biochemical aspects

Os alimentos funcionais têm sido empregados como adjuvantes no tratamento do câncer de mama. Neste estudo avaliaram-se as respostas hematológicas e bioquímicas clínicas à ação de um alimento funcional administrado a cadelas com diagnóstico de neoplasia mamária maligna. Após a mastectomia, 16 cadelas foram divididas em dois grupos: suplementadas (S) e não suplementadas (NS) com um composto comercial contendo Saccharomyces cerevisiae, mananoligossacarídeos e nutracêuticos. Ambos grupos receberam tratamento quimioterápico com doxorrubicina e carboplatina, alternadamente, em intervalos de 21 dias, por oito sessões, totalizando 168 dias de tratamento. As avaliações clínicas e laboratoriais foram realizadas nos momentos de aplicação do tratamento. Os resultados dos perfis hematológico (hemograma, leucograma e plaquetograma) e bioquímico sérico (ureia, creatinina, albumina, bilirrubina total e direta, alanina aminotransferase, fosfatase alcalina e gama glutamiltransferase - GGT) foram analisados pelo teste de Kruskall Wallis. No grupo S comprovou-se elevação do peso corporal e não foram observados transtornos gastrointestinais ou outros sinais de alteração clínica ao longo do tratamento. Diferentemente no grupo NS, ocorreu perda de peso e alterações clínicas, como diarreia e vômito. No quadro hematológico, constatou-se leucopenia por linfopenia no grupo de cadelas NS e preservação do valores dentro dos parâmetros considerados normais para a espécie no grupo S. Dentre todas as variáveis da bioquímica clínica, constatou-se apenas a elevação da atividade sérica da GGT nos animais do grupo NS, sem alterações no grupo S. Conclui-se que cadelas com neoplasia mamária quando suplementadas com com alimento funcional imunoestimulante apresentam melhor condição clínica, hematológica e dos níveis bioquímicos, particularmente da GGT.(AU)

Functional foods have been used as adjuvant for breast cancer treatment of bitches. The aim of the present study was to evaluate hematological and clinical biochemistry response in female dogs diagnosed with malignant mammary tumors and supplemented with functional food. After the mastectomy, 16 bitches were divided into two groups: supplemented (S) and none supplemented (NS) with a commercial product of Saccharomyces cerevisiae, mannanoligosaccharides and nutraceuticals. Chemotherapy with doxorubicin and carboplatin was performed alternately at intervals of 21 days for eight sessions during 168 days of treatment. Clinical and laboratorial assessments was made at the treatment moments. The results of the hemogram (erythrogram, leukogram and platelet count) and serum biochemistry (urea, creatinine, albumin, total and direct bilirubin, alanine aminotransferase, alkaline phosphatase and gamma glutamyltransferase - GGT) were analyzed by Kruskal Wallis test. In the S group, increase body weight was observed, but gastrointestinal disorders or other clinical disorders were not detected over the treatment. In the NS group, loss of weight and clinical disorders were observed. All hematology parameters were normal in the S group; however, leukopenia and lymphopenia were detected in the bitches of the NS group. Among all the clinical biochemistry parameters tested, only serum GGT was increased in the NS group, with no changes in the S group. In conclusion, female dogs with mammary tumor supplemented with immunostimulant functional food have better clinical condition, they demonstrate normal levels of hematological and biochemical exams, particularly GGT.(AU)

Coagulopatías adquiridas en pacientes ingresados en la Unidad de Cuidados Intensivos del Hospital Clínico Quirúrgico Hermanos Ameijeiras / Acquired coagulopathies in patients admitted to the Intensive Care Unit of Hermanos Ameijeiras Clinical-Surgical Hospital

Introducción: las coagulopatías adquiridas constituyen un problema de salud serio y frecuente en pacientes de las Unidades de Cuidados Intensivos, existe falta de evidencia para su tratamiento adecuado y eficaz. Es importante el reconocimiento temprano de estas anormalidades para reducir morbilidad, estadía hospitalaria, costos y mortalidad. Objetivos: describir el comportamiento de las coagulopatías adquiridas y las concentraciones de los factores de la coagulación (I, II, V, VII y X) en pacientes ingresados en la Unidad de Cuidados Intensivos del Hospital Clínico Quirúrgico Hermanos Ameijeiras. Métodos: se realizó un estudio longitudinal prospectivo desde abril hasta junio de 2011, en 29 pacientes ingresados en la Unidad de Cuidados Intensivos del Hospital Clínico Quirúrgico Hermanos Ameijeiras. Se determinaron los parámetros hematológicos al ingreso y cada 48-72 h para detectar coagulopatías adquiridas y déficit de factores de la coagulación. Resultados: presencia de coagulopatías al ingreso 58,62 por ciento, entre las 48-72 h 44,82 por ciento y en la última evaluación 51,73 por ciento. Predominó la coagulopatía por deficiencia de factores dependientes de vitamina K. Se encontró disminución significativa de los factores II, V, VII y X en el momento del ingreso; el II, V y X a las 48-72 h y en el momento final, los factores I y II. Conclusiones: se identificó la presencia de coagulopatías en diferentes momentos de la estadía dentro de la Unidad de Cuidados Intensivos, predominó la coagulopatía tipo 3: por consumo de factores dependientes de vitamina K. Hubo disminución significativa de la concentración de los factores dependientes de vitamina K(AU)

Introduction: Acquired coagulopathies constitute a serious and frequent health problem in patients at intensive care units. There is a lack of evidence for their adequate and effective treatment. Early recognition of these abnormalities is important to reduce morbidity, hospital stay, costs, and mortality. Objectives: To describe the behavior of acquired coagulopathies and the concentrations of coagulation factors (I, II, V, VII and X) in patients admitted to the Intensive Care Unit of Hermanos Ameijeiras Clinical-Surgical Hospital. Methods: A prospective longitudinal study was carried out from April to June 2011 in 29 patients admitted to the Intensive Care Unit of Hermanos Ameijeiras Clinical-Surgical Hospital. Hematological parameters were determined at admission time and every 48-72 hours in order to detect acquired coagulopathies and coagulation factor deficits. Results: Presence of coagulopathies on the admission was 58.62 percent, between 48-72 hours it was 44.82 percent and in the last assessment it was 51.73 percent. Prevalence of coagulopathy due to deficiency of vitamin K-dependent factors. Significant decrease in factors II, V, VII and X was found at the admission time; of the factor II, V and X after 48-72 hours, and of factors I and II in the final moment. Conclusions: The presence of coagulopathies was identified at different times of hospital stay within the intensive care unit, type 3 coagulopathy predominated: consumption of vitamin K-dependent factors. There was a significant decrease in the concentration of vitamin K-dependent factors(AU)

Efeito da administração pré-natal da dexametasona em ratas sobre os perfis glicídicos e hematológicos materno e da prole / Effect of prenatal administration of dexamethasone in rats on profiles hematologic and glicidic maternal and offspring

Avaliou-se o efeito da administração de dexametasona no início e no meio da gestação de ratas, sobre os perfis glicídicos e hematológicos materno e da prole. Os animais foram submetidos aos seguintes tratamentos: dexametasona do primeiro ao sétimo dia e placebo do oitavo ao 14º dia; placebo do primeiro ao sétimo dia e dexametasona do oitavo ao 14º dia; dexametasona do primeiro ao 14º dia e placebo do primeiro ao 14º dia de gestação. A dexametasona foi administrada por via intraperitoneal, na dose de 0,8mg/kg. Foram coletadas amostras de sangue no sétimo, 14º e 21º dias de gestação, e de sangue e tecido hepático da prole no quinto, 10º e 15º dias pós-natal. Para a verificação das reservas de glicogênio hepático da prole, cortes histológicos foram corados pelo ácido periódico de Schiff. Os resultados apontam para um efeito tempo-dependente da administração de dexametasona durante a gestação, levando a alterações temporais distintas na hematologia e na concentração plasmática de carboidratos nas matrizes e na prole.

The effect of the administration of dexamethasone at the beginning and middle of the pregnancy in rats on hematological and glicidic maternal and offspring profile was evaluated. The animals underwent the following treatments: dexamethasone from the first to the seventh day and placebo from the 8th to the 14th day, placebo from the first to the seventh day and dexamethasone from the 8th to the 14th day; dexamethasone from the 1st to the 14th day, and placebo treatment from the first to the 14th day of gestation. Dexamethasone was administered intraperitoneally at a dose of 0.8 mg/kg. Blood samples were collected on the 7th, 14th and 21st days of pregnancy, and blood and liver tissue of offspring on the fifth, 10th and 15th days postnatal. For verification of the reserves of liver glycogen on the offspring, histological sections were stained with periodic acid-Schiff. The results point to a time-dependent effect of dexamethasone during pregnancy, leading to different temporal changes in hematology and plasma levels of carbohydrates in headquarters and in the offspring.