RESUMO
Objective:To develop a method for the isolation and purification of suavissimoside R1 from Herb Rubi Parvifolii. Methods:Macroporous adsorption resin and preparative RP-HPLC,the water extract sedimentated by ethanol was pre-isolated by adsorption resin,and then fractionated by column chromatography on silica gel ( 100-200 mesh )using chloroform-methanol-water-acetic acid(40:10:1:0.4%).The collected fraction was purified for suavissimoside R1 by preparative RP-HPLC were combined as pre- pareation method.Results:Suavissimoside R1 was identified by mass spectrometry(MS),infrared spectrometry(IR),ultraviolet spectrometry(UV),nuclear magnetic resonance (NMR) and high performance liquid chromatography(HPLC),and compared with the relevant literature.The final purity of the compound was more than 98%.Conclusion:The developed method is simple,and reproducible.The solvent is cheap,low in boiling point and easily recovered.The product of suavissimoside R1 can be used as reference substance for analysis.