RESUMO
Objective: To observe the expressions of NMDAR2 and GLAST in the hippocampal gyrus and supraoptic nucleus(SON) under sleep deprivation in young rats.Methods: The changes in expressions of NMDAR2 and GLAST in SON and hippocampal gyrus under sleep deprivation in younger rats were observed by immunohistochemistry method. Results:The expressions of NMDAR2 and GLAST in SON and the hippocampal gyrus were significantly increased on the 3rd day of sleep deprivation,even more sigificantly on the 5th day,decreased gradually on 7th day,and became similar to those of control group on the 14th day.Conclusion: Sleep deprivation can affect the expressions of NMDAR2 and GLAST in the hippocampal gyrus and supraoptic nucleus,but the effect diminisshes gradually with prolonged time,which may be associated with self-regulation and self-protection of the central nervous system.
RESUMO
Objective To observe the effect of electric activity in the nucleus of solitary tract and the expression of NMDAR2 and GLAST in the zone of hippocampal gyrus after sleep deprivation in younger rats, in order to provide some theoretical basis for clinical therapeutie strategy. Methods Synchronous experiment was conducted with electrophysiological method. The changes in electric-activity of nucleus of solitary tract (NTS) were determined. Immunohistochemical method was employed to detected the expression of NMDAR2 and Glast in the zone of hippocampal gyrus in younger rats with sleep deprivation. Results The electric activity in nucleus of solitary tract (NTS) increased gradually along with sleep deprivation for 3 days, and the electric activity was much increased on the 7th day of sleep deprivation. In the second week of sleep deprivation, the electric activity in NTS was restrained. In the zone of hippocampal gyrus, the expression of both NMDAR2 and GLAST was increased significantly with 3-day sleep deprivation, and on the 5th day, the expression was much increased, while on the 7th day the expression decreased gradually. However, the expression of both NMDAR2 and GLAST in the zone of hippocampal gyrus in the second week of sleep deprivation was similar to that of the control group. Conclusion Sleep deprivation can exert a marked influence on the electric activity of the nucleus of solitary tract (NTS), and may interfere with the ability of study and memory in younger rats.