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Aim To explore how MCP-1 induces neu-rodisorder by determing the effects of MCP-1 on excita-tory postsynaptic current(EPSCs) in the CA1 region of rat hippocampal brain slices .Methods EPSCs, the AMPA receptor-mediated EPSC (EPSCAMPAR ), NMDA receptor mediated EPSCs(EPSCNMDAR) and NR2BR re-ceptor-mediated EPSC ( EPSCNR2BR ) were recorded u-sing whole-cell patch recording techniques to observe the effects of 2.3 nmol· L-1 MCP-1 on pyramidal neu-rons in hippocampal CA1 region.Microtubule-associat-ed protein-2 ( MAP-2 ) staining was used to study whether MCP-1 induced dendritic injuries in hippocam-pal CA1 region and whether NMDAR , AMPAR or CCR2 receptor antagonists had protective effects a-gainst dendritic damage caused by MCP-1.Results ① Bath application of MCP-1 produced a significant enhancement of the amplitudes of EPSCs , EPSCAMPAR and EPSCNMDAR .②Further studies revealed that MCP-1 potentiated EPSC NR2BR; ③ The MCP-1-associated dendritic injuries were blocked by NMDAR , AMPAR and CCR2R antagonists respectively .Conclusions Our results suggest a potential role of MCP-1 which may play in neuroexcitotoxicity and neural injury via NMDA receptor(especially NMDAR subtype NR2BR) and CCR2 receptor .The antagonists of these receptors may have potential therapeutic effect for neurodegener-ation.
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AIM: To study the effects of huperzine A on the theta-rhythm and long-term potentiation in CA1 pyramidal neurons in adult rat hippocampal slices and to get insight into the cell electrophysiological mechanisms underlying the potentiation of learning and memory by Huperzine A. METHODS: The intracellular recordings from CA1 pyramidal neurons in hippocampal slices related to learning and memory were made to analyze the mechanisms of Hup-A action. RESULTS: Power of frequencies ranged from 4 to 10 Hz was not significantly difference between pre-membrane potential oscillations (MPOs) and post-MPOs in control group, but it was significantly (P
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Objective:To explor the effect of gp120mAb on synaptic transmission and plasticity changed by gp120 in the CA 1 region of rat hippocampal slices.Methods:We recorded the excitatory postsynaptic potentials (EPSP) and investigated effect of gp120mAb on long-term potentiation inhibited by gp120 in rat hippocampus in vitro.Results:The results showed that HIV-1 gp120 can inhibit the LTP induced by high-frequence stimulation schaffer in CA 1,gp120 had no effect on basal synaptic transmission.HIV-1 gp120(200 pmol/L) can inhibit the LTP maintance,gp120mAb of gp120-specific antibodies can reverse the inhibition effect,But IgG can't reverse it.Conclusion:The reverse effect of gp120-specific antibody gp120mAb on long-term potentiation inhibited by HIV-1 gp120 may contribute to the HAD pathogenesis.