Desnutrición asociada a la parasitosis intestinal en la etnia indígena Hiwi de San Juan de Manapiare, amazonía venezolana / Malnutrition associated with intestinal parasitosis in the indigenous Hiwi ethnic group of San Juan de Manapiare, Venezuelan Amazon

Es de conocimiento común que la desnutrición afecta preferentemente a países en vías de desarrollo, donde constituye una causa muy frecuente de morbi-mortalidad en niños y adultos, con predilección en las poblaciones más desfavorecidas. El siguiente estudio descriptivo se realizó en la comunidad indígena Hiwi de San Juan De Manapiare en el Amazonas venezolano con el objetivo de establecer el tipo de parasitismo intestinal más frecuente en dicha población y su relación muy estrecha con la desnutrición y diferentes grados anemia. Se estudiaron las muestras de heces recién emitidas y se examinaron en el microscopio, asimismo se le realizo el examen físico a los participantes del estudio y se utilizaron los datos antropométricos obtenidos para hacer la pertinente comparación con diferentes métodos de referencia para estimar el grado de desnutrición de cada individuo, de igual modo se extrajeron las muestras de sangre para determinar el nivel de hematocrito, hemoglobina, la formula total y diferencial de los leucocitos. Se obtuvo como resultado principal que los helmintos son los parásitos más frecuentes en la población estudiada. El presente trabajo pretende dar una imagen real de las patologías que más afectan a las comunidades originarias en el contexto actual de los derechos indígenas con el objetivo de avivar el interés por las investigaciones en dicha población y de aportar ideas para realizar planes concretos a largo plazo para tratar de mitigar los efectos tan deletéreos que tienen en la salud las enfermedades infecciosas(AU)

It's common knowledge that malnutrition preferentially affects developing countries, where it constitutes a very frequent cause of morbidity and mortality in children and adults, with a predilection in the most disadvantaged populations. The following descriptive study was carried out in the Hiwi community of San Juan De Manapiare in the Venezuelan Amazon with the objective of establishing the most frequent type of intestinal parasitism in said population and its very close relationship with malnutrition and different degrees of anemia. Freshly passed stool samples were studied and examined under the microscope, likewise a physical examination was performed on the study participants and the anthropometric data obtained were used to make the pertinent comparison with different reference methods to estimate the degree of malnutrition of each individual, in the same way, blood samples were extracted to determine the level of hematocrit, hemoglobin, the total and differential formula of leukocytes. The main result was that helminths are the most frequent parasites in the studied population. The present work tries to give a real image of the pathologies that most affect the original communities in the current context of the rights of the indigenous with the aim of stimulating interest in research in this population and contributing ideas to carry out concrete long-term plans period to try to mitigate the deleterious effects that infectious diseases have on health(AU)

The effect of inhibition of hiwi expression by RNAi on triple negative breast cancer cells MDA-MB-231 through inducing cell apoptosis / 实用医学杂志

Objective To investigate the effect of interfering Hiwi gene on the apoptosis of MDA-MB-231 cells. Methods The mRNA and protein expression of Hiwi mRNA and its target protein were analyzed by qRT-PCR and Western Blot after transfection. MDA-MB-231 cells were divided into 6 groups according to the experimental design. Interference effects were screened as siRNA interference group (Hiwi10330 group), and then divided into 3 groups according to the experimental design: interference group, negative control group/NC, blank control group/Blank. The cell apoptosis rate was detected by flow cytometry after transfection. Results The expression of mRNA in the interference group was significantly lower than that in the siRNA group (P < 0.05), the expression of target protein of Hiwi gene was also significantly inhibited (P < 0.05). The apoptosis rate of MDA-MB-231 cells was significantly higher than that of NC and Blank groups (P<0.05). Conclusion The apoptosis rate of breast cancer cells MDA-MB-231 was significantly increased after siRNA targeting hiwi gene silencing.

Construction of recombinant adenovirus vector pAV.Ex1 d-Hiwi using Gateway technology / 吉林大学学报(医学版)

Objective To obtain the Hiwi gene encoding full-length and construct human Hiwi adenoviral vectors carrying green luorescence protein (GFP), and to establish foundation for a further study on Hiwi function and mechanism of inducing leukemia stem cell differentiation and apoptosis.Methods All coding areas of human Hiwi gene full length were amplified using method of overlapping extension PCR technology, and the full length coding aeras were inserted into the vector of Flag-IRES-hrGFP carrying GFP with Gateway technology to construct pDown-Hiwi-3 × flag-IRES-hrGFP. The cloning vector pDown-Hiwi-3 × flag-IRES-hrGFP and expression vector pAV. Des1d were used for homologous recombination reaction to obtain recombinant adenovirus vector pAV.Ex1d-Hiwi-3× flag-IRES-hrGFP.The positive clones were selected by PCR to extract the recombinant adenovirus plasmid and to pack into recombinant Ad-Hiwi-3 × flag-IRES-hrGFP adenovirus. Results The human recombinant Hiwi was successfully cloned and the recombinant adenovirus vector pAV.Ex1d-Hiwi-3×flag-IRES-hrGFP was found to be successfully constructed via restriction enzyme digestion and sequencing methods. The adenovirus vector pAV.Ex1d-Hiwi-3×flag-IRES-hrGFP was transfected into the HEK293A cells using lipofectamine mediated gene transfection method. Under fluorescence microscope, the transfected cells with green fluorescence could be observed.Conclusion The expression plasmid of adenovirus vector pAV.Ex1d-Hiwi-3 × flag-IRES-hrGFP is successfully constructed and it can express in HEK293A cells.

Influence of HIWI gene silencing in biological behavior of bladder carcinoma cells / 吉林大学学报(医学版)

Objective To investigate the influence of HIWI gene silencing in biological behavior of T24 cells and explore the possibility for HIWI gene to be used as the molecular target of inhibiting bladder carcinoma cell proliferation with gene transfection and RNA interference(RNAi) technique.Methods T24 cells were divided into transfection group with pGenesil-2-HIWI,transfection group with pGenesil-2-HIWI2263,transfection group with pGensil-2-control,and two control groups transfected with PEI only,and PBS only,respectively.T24 cells were transfected with shRNA expression vectors targeting HIWI gene by PEI,and the cell proliferation and cell cycle were measured by MTT assay and FCM.Results At the time of post-transfection 24 h,the inhibitory rate of cell proliferation in transfection groups were 32.60% and 26.09%,they were lower than that in control group(3.54%).At the time of post-transfection 48 h,the percentages of cells at S phase in transfection groups were(29.39? 3.27)% and(30.87?10.88)%,they were lower than that in control group(39.36%?2.09%)(P