RESUMO
OBJECTIVE: To establish a quantitative method for determining the contents of uracil, cytidine, hypoxanthine, xanthine, uridine, inosine, and guanosine in Holotrichia diomphalia Larvae by HPLC. METHODS: The HPLC analysis was performed on Waters HSS T3 column(4.6 mm×250 mm, 5 μm). The mobile phase was composed of acetonitrile(A) and water(B), and gradient elution was carried out. The flow rate was 1.0 mL•min-1. The column temperature was maintained at 30 ℃. The detection wavelength was 260 nm. RESULTS: The correlation coefficients of the seven components ranged from 0.999 1 to 1.000 0. The average recovery rates(n=6) were between 91.2% and 97.4%, and the RSDs were between 1.5% and 2.3%. CONCLUSION: The proposed method is simple, accurate and reliable, thus providing basis for comprehensive quality control of Holotrichia diomphalia Larvae.
RESUMO
Objective To analyze the chemical components of the liposoluble constituent of Holotrichia diomphalia lar-vae .Methods Essential liposoluble compounds were extracted from H .diomphalia through ethylacetate .GC-MS analysis was used to identify its main components .Results 33 peaks were separated and 33 compounds were identified from ethylacetate-ex-tracted H .diomphalia .The main compounds were pentadecylic acid ,Z-11-Hexadecenoic acid ,hexadecanoic acid ,heptade-canoic acid ,oleic acid and octadecanoic acid .Conclusion This work will contribute to the study of medicinal animals and con-firmation of active compounds in H .diomphalia .