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Objective: To search for effective volatile oil of Chinese crude drug for killing demodex in vitro. Methods: The demodex mites were collected by adhesive cellophane tape technique. The killing effects of different volatile oils against Df and Db were observed by microscope. Normal saline and Xinfu Manling Ointment were used as negative and positive controls, respectively. Skin irritation tests for normal and wounded skin and acute toxicity test were carried out using rabbit skin. Results: The volatile oils of 6 Chinese crude drugs, including clore, orange fruit, manchurian wildginger, cinnamon bark, rhizoma alpinae, and pricklyash peel, were highly potent in killing Df and Db in vitro; and the volatile oil of clore and manchurian wildginger had a better killing effect against Df than Db(P< 0.05). The scores for irritation to normal and wounded rabbit skin were 0.2 and 0.3, respectively, and acute toxicity test showed no obvious toxicity. Conclusion: The volatile oils of the 6 mentioned Chinese crude drugs have satisfactory anti-mite activities in vitro. The volatile oil of clore shows a remarkable activity against demodex in vitro, with good skin safety.
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Objective To observe the effect of the volatile oil of zanthoxylum bungeanum in killing demodex in vitro.Method The mites were collected with adhesive cellophane tape technique.The killing effect of the pure volatile oil with different concentrations volatile oils on human demodex was investigated by microscope.Results The pure volatile oil was highly powerful in killing D.f and D.b in vitro,and for the D.b,the killing effect was better.With the increase of dilutus multiple,the killing effect dareased.Conclusion The pure volatile oil of zanthoxylum bungeanum is an effective component in killing demodex in vitro.
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This article summarizes the present researches on biology of human Demodex about the methods of examination,morphology of adult parasite,biological habits and characteristics,and in vitro survival.
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Objective To understand the difference of Demodex prevalence among medical students living and studying together with different classes, sexes and nationalities. \ Methods\ Demodex folliculorum and Demodex brevis were detected by using cellophane tape on the nasolabial grooves. \ Results\ The overall prevalence was 51^5% in 2 248 medical students. Prevalence in different classes and sexes was: freshman 42^6%, sophomore 49^6% and third year students 66^8%; 55^7% in males and 48^7% in females. Prevalence in students with different nationalities was: Mongolian 49^8%, Han 52^8% and other minorities 64^3%. \ Conclusion\ The Demodex prevalence in students of senior classes was higher than those from junior ones (P