RESUMO
OBJECTIVE: To establish a rapid approach for quality evaluation of Huoxiang Zhengqi Tincture. METHODS: Fingerprint was established by ultra performance liquid chromatography (UPLC) method and method evaluation was performed. By comparing with reference substances, reference drugs and reference extractives, the characteristic peaks and their ascriptions were investigated. Twenty-eight batches of samples from 13 manufactures were determined and their fingerprints were compared. Then two dimensional hierarchical cluster analysis (2D-HCA) and principal analysis (PCA)were applied in pattern recognition. RESULTS: Eighteen characteristic peaks were mainly related to Magnoliae Officinalis Cortex, Citri Pericarpium Reticulatae, Angelicae Dahuricae Radix, Extractum Glycyrrhizae, Atractylodis Rhizoma and patchouli oil. The UPLC fingerprints of samples from different manufacturers differed a lot. The results of 2D-HCA and PCA were consistent, which indicated that the fingerprints of the samples from the same manufacturer could cluster to their own group and the characteristic peaks of honokiol, magnolol and hesperidin showed the greatest impact on the fingerprints. CONCLUSION: UPLC fingerprinting with aid of chemometric analysis is rapid, simple, objective and easy to realize digitalization, thus providing a novel way for quality evaluation of Huoxiang Zhengqi Tincture.
RESUMO
OBJECTIVE: To investigate a rapid approach for quality evaluation of Huoxiang Zhengqi Tincture. METHODS: Hesperidin, glycyrrhizic acid, imperatorin, honokiol, isoimperatorin, magnolol and atractylodin in Huoxiang Zhengqi Tincture were determined by ultra performance liquid chromatography (UPLC) method combined with wavelength switching detection technology. The sample was injected directly without preprocessing. The separation was performed on an ACQUITY UPLC BEH C18 (2.1 mm×100 mm, 1.8 μm) and the column temperature was maitained at 40℃. The mobile phase was composed of acetonitrile and 0.1% phosphoric acid with gradient elution at a flaw rate of 0.3 mL·min-1. Hesperidin was detected at 284 nm; glycyrrhizic acid was detected at 250 nm; imperatorin, honokiol, isoimperatorin and magnolol were detected at 300 nm; atractylodin was detected at 340 nm. RESULTS: The calibration curves of the seven components showed good linearity within their test ranges. The average recoveries for hesperidin, glycyrrhizic acid, imperatorin, honokiol, isoimperatorin, magnolol and atractylodin were 99.3%, 99.5%, 101.5%, 99.3%, 100.6%, 99.0% and 99.6%, respectively. And there were great variations among the contents of glycyrrhizic acid, imperatorin, isoimperatorin and atractylodin in 28 batches of samples from 13 manufactures. CONCLUSION: The proposed method is accurate, simple and rapid, thus providing basis for comprehensive quality control of Huoxiang Zhengqi Tincture.
RESUMO
Objective A headspace-gas chromatography method was established for determination of methanol in Huox-iang Zhengqi Tincture. Methods Headspace-gas chromatography was performed on a HP-INNOWAX capillary col-umn, with nitrogen as carrier gas and FID as detector. The injector temperature was at 150℃ and the detector tempera-ture was at 200℃ , and temperature programming and split stream sampling were used. Results A good linearity was obtained within the designed range, the average recovery was 98.89 % with the RSD of 1.36 %, which met the correla-tive criterion of China Pharmacopoeia. Conclusion This method is proved to be accurate, and it has a good reproducibil-ity and can be used to determinate the residual methanol in Huoxiang Zhengqi Tincture.