Extraction of Essential Oil from a Leaf of Piper betle L. (Paan) by Hydro-distillation Method

Piper betle leaf commonly known to be a Paan in India is eaten raw and sometimes with a mixture of areca nut. The leaf is consumed usually after a lunch or dinner. Traditionally the leaf is well known for a health benefits and also chewed as a mouth freshener. A wide range of researches on a Piper betle leaf suggest excellent nutritional benefits on the health. The edible leaf is also known for its specialized essential contents. The current research attempt is carried out to extract the essential oil from the leaf of Piper betle L. by a Hydro distillation method (Clevenger method). The research successfully concludes the extraction of oil with its efficacy towards the percentage of oil achieved during an extraction process.

GC-MS Compositional Analysis of Essential Oil of Leaf and Fruit Rind of Citrus maxima (Burm.) Merr. from Coastal Karnataka, India.

Essential oils derived from plants by hydro distillation possess several medicinal properties. Citrus maxima (Burm.) Merr. fruit is nutritive, cardiotonic, refrigerant, in stomach troubles, puerperal sepsis and insanity; while leaves are useful in epilepsy, chorea, convulsive cough and stomach pain due to indigestion. Gas chromatography coupled with mass spectroscopy (GCMS) has been used for detection and identification of volatile components from the hydro-distillate from leaf and rind of Citrus maxima (Burm.) Merr. of Rutaceae. Yield of oil was more in rind (0.12%) compared to leaf (0.07%). Forty two and thirty four compounds were detected from leaf and fruit respectively, among which citronellol (28.26%) was the major component in the leaves while D-limonene (89.04%) in the rind. Volatile compounds responsible for various medicinal properties are identified from hydro-distillate of Citrus maxima (Burm.) Merr. from Dakshina Kannada using GC-MS.

Atividade antimicrobiana do óleo essencial de Cymbopogon citratus / Antimicrobial activity of Cymbopogon citratus essential oil / Actividad antimicrobiana del aceite esencial de Cymbopogon citratus

O Cymbopogon citratus é uma planta originária da Índia, pertencente à família Poaceae e facilmente adaptada em regiões tropicais como o Brasil. Este trabalho avaliou a atividade antimicrobiana do óleo essencial (OE) obtido por hidrodestilação das folhas de C. citratus. A concentração inibitória mínima (CIM) foi determinada pela técnica de microdiluição em caldo para as bactérias Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa e a levedura Candida albicans. O menor CIM foi de 7,81 µg mL-1 para S. aureus, a média de 15,62µg mL-1 para P. aeruginosa e C. albicans e a maior de 62,50µg mL-1 para E. coli. O óleo essencial de C. citratus apresentou atividade antimicrobiana para todos os micro-organismos testados. O óleo mostrou potencial aplicabilidade no controle microbiano e sugerem-se novos estudos para auxiliar na ampliação das aplicações desta planta...

Cymbopogon citratus is an Indian plant belonging to the Poaceae family, easily adapted to tropical regions such as Brazil. This study assesses the antimicrobial activity of essential oil from C. citratus leaves obtained by hydro-distillation. The minimum inhibitory concentration (MIC) was determined by broth micro-dilution technique for Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. The lowest MIC was 7.81 µg ml-1 for S. aureus, the medium was 15,62 µg mL-1 for P. aeruginosa and C. albicans and the highest was 62.50 µg mL-1 for E. coli. The C. citratus essential oil presented antimicrobial activity against all microorganisms. The essential oil showed potential applicability in microbial control and further studies are suggested to expand the application of this plant...

Cymbopogon citratus es una planta originaria de India, perteneciente a la familia Poaceae y fácilmente adaptable en las regiones tropicales como Brasil. Este estudio evaluó la actividad antimicrobiana del aceite esencial de las hojas de C. citratus obtenido por hidrodestilación. La concentración inhibidora mínima (CIM) se ha determinado por la técnica de microdilución en caldo para las bacterias Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa y la levadura Candida albicans. La CIM más baja fue 7.81 µg mL-1 para S. aureus, la media de 15.62µg mL-1 para P. aeruginosa y C. albicans y la mayor de 62.50 µg mL-1 para E.coli. El aceite esencial de C. citratus presentó actividad antimicrobiana para todos los microorganismos testados. El aceite mostró potencial de aplicabilidad en el control microbiano y se sugieren nuevos estudios para ampliar las aplicaciones de esta planta...

Comparison of volatile compounds in teucrium polium l. by headspace and hydrodistillation techniques.

Aerial parts of Teucrium polium were subjected to headspace (HS) and hydro-distillation (HD) techniques after drying, then headspace volatiles and the essential oil were analyzed by GC/MS. Forty-eight and Thirty-nine constituents were identified in hydro-distillation and CombiPAL system which represented 99.7% and 99.8% of the oils, respectively. hydro-distillation method were α-pinene (30.8%), β- pinene (12.0%), myrcene (8.9%), limonene (7.9%), (E)-caryophyllene (5.6%), Germacrene D (6.9%), Bicyclogermacrene (4.5%), α- Eudesmol (2.0), valerianol (3.4%), and 7-epi-α-Eudesmol (3.2%) and the main compounds by CombiPAL techniques were α-pinene (38.8%), β- pinene (15.5%), myrcene (21.0%), limonene (13.1%), and trans- β- ocimene (3.4 %). The Headspace Technique is a new, rapid, simple and eco-friendly method for essential oil analysis of aromatic plants.