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Objective:To investigate the significance of expression of serum soluble HLA-G (sHLA-G), alpha-hydroxybutyrate dehydrogenase (α-HBDH) and signal transducer and activator of transcription 1 (STAT1) in acute myeloid leukemia (AML) and their relationship with prognosis.Methods:A total of 107 patients with AML who received treatment in Xiaoshan Hospital, Wenzhou Medical University, from June 2016 to June 2019 were included in the AML group. An additional 100 healthy controls who concurrently received physical health examination in the same hospital were included in the control group. Serum samples were collected from both patients with AML and healthy controls. Serum concentration of sHLA-G concentration was determined using the double-antibody sandwich enzyme-linked immunosorbent assay. Serum α-HBDH concentration was determined using the rate method. Peripheral blood was collected from patients with AML and healthy controls. The relative expression of STAT1 was determined by quantitative real-time polymerase chain reaction.Results:sHLA-G and α-HBDH concentrations in the AML group were (13.26 ± 2.19) μg/L and (362.17 ± 38.47) U/L, respectively, which were significantly higher than those in the control group [(5.08 ± 0.43) μg/L, (108.94 ± 12.19) U/L, t = 36.69, 62.93, both P < 0.05]. The relative expression of STAT1 in the AML group was significantly higher than that in the control group [(3.17 ± 0.25) vs. (1.01 ± 0.01), t = 86.32, P < 0.05] Among patients with Hb ≤ 80 g/L, the number of patients with high sHLA-G expression was higher than that of patients with low sHLA-G expression ( χ2 = 7.15, P < 0.05). The number of patients with white blood cells > 10 × 10 9/L was significantly higher than that of patients with low sHLA-G expression ( χ2 = 17.31, P < 0.05). Among patients with Hb ≤ 80 g/L, the number of patients with high α-HBDH expression was significantly higher than that of patients with low α-HBDH expression ( χ2 = 10.76, P < 0.05). The number of patients with white blood cells > 10 × 10 9/L was significantly higher than that with low α-HBDH expression ( χ2 = 13.17, P < 0.05). Among patients with Hb ≤ 80 g/L, the number of patients with high STAT1 expression was significantly higher than that of patients with low STAT1 expression ( χ2 = 18.14, P < 0.05). The number of patients with white blood cells > 10 × 10 9/L was significantly higher than that of patients with low STAT1 expression ( χ2 = 18.51, P < 0.05). The overall survival time in patients with high sHLA-G, α-HBDH expression and STAT1 expression was (17.92 ± 1.72) months, (19.34 ± 1.57) months, and (16.36 ± 2.08) months, respectively, which were significantly lower than those in patients with low sHLA-G, α-HBDH and STAT1 expression [(28.93 ± 1.46) months, (27.53 ± 1.89) months, (30.48 ± 1.12) months, t = 35.08, 24.07, 38.32, all P < 0.05]. Conclusion:sHLA-G, α-HBDH and STAT1 are abnormally expressed in patients with AML. Higher sHLA-G, α-HBDH and STAT1 expression indicates poorer prognosis of AML. Therefore, sHLA-G, α-HBDH and STAT1 can be used as the potential targets for AML treatment and prognosis prediction. Findings from this study are highly innovative and scientific.
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Objective This study analyzed the expression of alpha-hydroxybutyrate dehydrogenase (α-HBDH) in serum and explored its predicative value in the diagnosis of ovarian cancer (OC). Methods A retrospective study was conducted on 319 OC patients (OC group), 400 patients with benign lesions (benign group), and 400 healthy controls (normal group). These subjects were treated or received physical examination in Women's Hospital, School of Medicine, Zhejiang University from January 2014 to August 2018. Each group was further stratified by menopausal status. The expression levels ofα-HBDH and carbohydrate antigen125 (CA125) and their associations with clinic-pathological characteristics of OC were evaluated, and their diagnostic efficacy in OC were explored. Wilcoxon Rank Test and Kruskal-Wallis H test were used for group comparison. Receiver operating curve(ROC) was plotted to evaluate the diagnostic capability of α-HBDH and CA125 in OC. Results The median of α-HBDH level was 134.0 U/L in OC group, 120.0 U/L in benign group, and 110.0 U/L in normal group. OC group was significantly different from other two groups (H=129.5, P<0.001). Serumα-HBDH was also correlated with the menopausal status, lymph node metastasis, and clinical stage of OC patients significantly(Z=-5.2, H=31.5,Z=-3.2,all P<0.001). In the ROC analysis in terms of OC risk, the area under curve (AUC) ofα-HBDH was lower than AUC of CA125 [premenopausal group (α-HBDH: AUC=0.685; CA125: AUC=0.796;menomenopausal group (α-HBDH:AUC=0.749;CA125:AUC=0.915)];and in the stage I of premenopausal group, α-HBDH performed similar to CA125, but with obviously higher sensitivity than CA125 (α-HBDH:AUC:=0.646, Se=79.41％, SP=41.61％;CA125:AUC=0.691, Se=58.82％, Sp=74.71％). Conclusions The expression level of serum α-HBDH level was increased in OC patients, and it was associated with menopausal stage, lymph node metastasis, and clinical stage of OC. In addition, α-HBDH showed higher sensitivity than CA125 in stage I premenopausal group, which was potentially beneficial for the diagnosis of stage I OC in premenopausal women.
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Objective To explore the influence of the serum liver function,α-glutathione S-transferase(α-GST) and α-hydroxybutyrate dehydrogenase(α-HBDH) on pregnancy outcome in patients with intrahepatic cholestasis of pregnancy(ICP).Methods Retrospective analysis was conducted on the clinical data of 80 cases with ICP who admitted in our hospital from January 2013 to December 2015.The patients were divided into mild group and severe group.Biochemical indicators and the indicators reflecting poor pregnancy outcomes were analyzed.ResultsThe levels of alanine transferase(ALT),aspartate transferase(AST),total bile acid(TBA) and direct bilirubin(DB) in the severe group were significantly higher than those in the mild group(t=4.07,3.45,3.50,2.11,P=0.04,P＜0.01).The incidence rate of fetal distress,premature delivery and pollution of amniotic fluid above II degree was significantly higher in the severe group(χ2=8.352,all P＜0.01).There were no significant differences in gestational weeks,cesarean section rate,the amount of postpartum hemorrhage and neonatal birth weight between the two groups (χ2=4.178,P=0.892).Logistic regression analysis showed that gestational weeks,neonatal birth weight and the levels of TBA,AST,ALT,α-GST and α-HBDH were associated with the severity of ICP(all P＜0.01).α-GST had higher sensitivity and specificity in predicting adverse perinatal outcomes,such as amniotic fluid,premature rupture of membranes,preterm birth,low birth weight and fetal distress.Conclusion The perinatal outcomes of pregnant women with severe ICP can be improved by grading management according to their serum biochemical indicators and active clinical intervention treatment.α-GST and α-HBDH can be used to evaluate the severity of ICP,which have higher clinical value in predicting poor pregnancy outcomes.
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Objective:To study the curative efficacy of decitabine combine with CAG regimen in the treatment of acute myeloid leukemia and its effects on the serum Interferon-γ (IFN-γ),alpha hydroxybutyrate dehydrogenase (HBDH) and lactate dehydrogenase (LDH) levels.Methods:70 cases of patients with acute myeloid leukemia who were treated from February 2013 to October 2016 in our hospital were selected as research objects.According to the random number table,the patients were divided into the observation group (n=35) and the control group (n=35).Both groups of patients were treated conventional treatment.The control group was treated with CAG scheme,intravenous injection of aclarubicin,20 mg each time,granulocyte colony stimulating factor,300 μg each time,subcutaneous intravenous cytarabine,10～15 mg/m2 each time,while the observation group was treated with intravenous drip of decitabine on the basis of control group,15 mg/m2 each time.Then the therapeutic effect,serum interferon-γ (IFN-γ),alpha hydroxybutyrate dehydrogenase (HBDH),lactate dehydrogenase (LDH) levels before and after treatment,incidence of adverse reactions were compared between two groups.Results:After treatment,the total effective of observation group was significantly higher than that of the control group [91.42％(32/35)vs68.57％(24/35)] (P ＜0.05);the serum IFN-γ,HBDH,LDH levels were significantly higher than those of the control group [(3.21± 1.01)pg/ml vs.(5.13 ± 1.90)pg/mL,(103.62± 26.39)U/L vs.(118.80± 28.60)U/L,(101.36± 27.32)U/L vs.(123.08 ± 30.59)U/L] (P ＜0.05);the incidence rate of adverse reactions was significantly lower than that of the control group [20.00％(7/35) vs.42.85％(15/35)(P ＜0.05)].Conclusion:Decitabine combined with CAG regimen was more effective for acute myeloid leukemia than CAG regimen alone,which might be related to reduce the serum levels of IFN-γ, HBDH and LDH.
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The objectives of this study are as follows:1) to explore the expression level of alpha hydroxybutyric acid dehydrogenase (α-HBDH) in patients with non-Hodgkin's lymphoma (NHL) and its prognostic significance;and 2) to analyze the relationship amongα-HBDH, lactate dehydrogenase (LDH), and beta 2-microglobulin (β2-MG), so as to evaluate their diagnostic and prognostic sig-nificance. Methods:The expression levels of serumα-HBDH, LDH, andβ2-MG were examined in 104 patients with NHL before and after treatment. The relations among the levels of serum LDH,α-HBDH, andβ2-MG, as well as their connection with the patients' age, gender, disease stage, and pathological type, were explored. Results:Serumα-HBDH level increased in 35%of the patients and showed a significant correlation with LDH andβ2-MG. Significant differences were observed forα-HBDH andβ2-MG at different stages but not for LDH. Significant differences were observed betweenα-HBDH and LDH for different pathological types, but none was found inβ2-MG. The three serum enzymes did not exhibit any significant difference for different ages and genders. Levels of serum LDH andα-HBDH showed considerable difference between pre-treatment and post-treatment of patients. Serumβ2-MG level did not show any significant change after two or three cycles of chemotherapy. Conclusion:The expression level of serumα-HBDH increases in patients with NHL, is positively correlated with the levels of LDH andβ2-MG, and is highly relevant to disease stage and pathological type, regardless of the patients' age and gender. Serumα-HBDH is expected to be a new NHL indicator for tumor load, disease severity, and prognosis.
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Objective To analyze the clinical characteristic of autoimmune gastritis (AIG).Methods From January 1990 to April 2010,the clinical data of 55 AIG patients were retrospectively analyzed,which included hemoglobin,lactate dehydrogenase (LDH),α-hydroxybutyrate dehydrogenase (α-HBDH),gastrin,intrinsic factor antibody (IFA),parietal cell antibody (PCA),gastrointestinal endoscopy examination and 24-hour esophageal pH recording.Another 31 megaloblastic anemia (MA) patients were selected as control.Statistical analysis was performed by independent-samples t test.Results Among 55 AIG patients,49 patients were associated with MA,and three out of four cases were identified of IFA.About 43.8％ (21/48) patients were PCA positive.Before treatment,the levels of LDH and α-HBDH of AIG patients with MA were (1045.50±853.46)U/L and (853.71±824.23) U/L which significantly increased,than those of patients without MA [(166.67±41.03) U/L,(133.67±27.90) U/L],the differences were statistically significant (t=-4.665,-2.120,both P＜0.05),however there was no significant difference when compared with the control group [(1047.52±1028.31) U/L,(1050.23±1264.37) U/L,both P＞0.05)].A total of 46 patients underwent gastroendoscopy examination,63.0％ (29/46) patients had gastric body atrophy while gastric antrum not involved; 34.8％ (16/46) patients had neither gastric body nor antrum atrophy; seven patients gastric mucosal showed intestinal metaplasia and one patient showed intestinal metaplasia with atypical hyperplasia and 2.2％ (1/46) presented both the antrum and the body atrophy.Conclusions The levels of LDH and α-HBDH increased in AIG patients might be related with MA caused marrow in-situ hemolysis.IFA is recommended as a routine test for AIG.There is still some limitations of AIG diagnosis according to histopathological features of gastric endoscopy specimen.The clinical features should be taken into consideration.
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ObjectiveTo investigate the changes of serum myocardial enzymes and high density lipoprotein cholesterol(HDL-C) in children with septic shock.MethodsFifty-two children with septic shock in our hospital from Jan 2006 to Mar 2011 were divided into severe septic shock group( n =27 ) and mild septic shock group ( n =25 ).The serum creatine kinase-MB ( CK-MB ),α-hydroxybutyrate dehydrogenase (α-HBDH),lactic dehydrogenase(LDH) and HDL-C were analysed in both two groups on 1,2,4,7,10,15 d,and compared with 32 normal children as control group.Meanwhile,the children's condition,death cases and cured time were recorded.ResultsThe levels of serum CK-MB [ ( 32.084 ± 4.595 ) U/L,( 61.481 ±5.639) U/L],α-HBDH/LDH(0.694 ±0.080,0.884 ±0.079) in mild and severe septic shock groups were higher than those of normal control group[ (21.675 ± 3.453) U/L ] and (0.443 ± 0.065 ) ( P ＜ 0.01 ) ;the levels of serum HDL-C[ (0.646 ±0.067) mmol/L,(0.310 ± 0.124) mmol/L ] in the two septic shock groups were decreased significantly than that of control group[ ( 1.012 ±0.156) mmol/L] (P ＜0.01 ).Five cases died in the severe septic shock group while no cases died in mild septic shock group.The times of CK-MB recovered[ (9.82 ±1.76) d vs (4.68 ± 1.22) d],α-HBDH/LDH recovered[ (7.23 ± 1.38) d vs (3.76 ± 0.83 ) d] and HDL recovered[ ( 12.14 ± 2.21 ) d vs ( 6.48 ± 1.33 ) d ] were more slowly in severe septic shock group than those of mild septic shock group( P ＜ 0.01 ).ConclusionThe higher of the serum CK-MB and α-HBDH/LDH,and the lower of HDL-C in septic shock children,the disease is more severe,the recovery time is longer and a worse prognosis may occur.