Bone regeneration of the fluoridated hydroxyapatite and the bio-glass in the rabbit cranium defect model

INTRODUCTION: Hydroxyapatite (Ca10(PO4)6(OH)2, HA) is the main inorganic phase of human hard tissue that is used widely as the repair material for bones. When HA is applied to a bony defect, however, it can be encapsulated with fibrous tissue and float in the implanted area due to a lack of consolidation. Bioceramics as allogenic graft materials are added to HA to improve the rate and bone healing capacity. Fluoridated hydroxyapatite (Ca10(PO4)6(OH,F)2, FHA), where F- partially replaces the OH- in hydroxyapatite, is considered a good alternative material for bone repair owing to its solubility and biocompatibility. MATERIALS AND METHODS: This study was designed to determine the bone healing capacity of FHA newly produced as a nanoscale fiber in the laboratory. HA and FHA with bioglass was implanted in a rabbit cranium defect and the specimen was analysed histologically. RESULTS: 1. At 4 weeks, fibrous connective tissue and little bone formation was observed around the materials of the experimental group I implanted HA and bioglass. Newly formed bone was observed around the materials in the experimental group II implanted FHA and bioglass. 2. At 8 weeks, the amount of newly formed and matured bone was higher in experimental group II than in experimental group I and the control group. CONCLUSION: These results suggest that FHA and bioglass is a relatively favorable bone substitute with biocompatibility and better bone healing capacity than pure HA and bioglass.

Preenchimentos faciais - parte um / Dermal fillers - part one

Nos últimos anos, presenciamos o aparecimento de grande variedade de preenchimentos dérmicos. Eles permitiram que aumentasse nossa variedade de escolha, nunca tida antes. Neste artigo abordaremos algumas novas opções de preenchedores.

Application of Hard Tissue Filler for Chin Contouring

With hightened interest in bone contouring surgeries, newer and lesser invasive treatment modalities for bone augmentation surgeries are being introduced. Bone augmentation effects with long lasting injectable tissue fillers were studied. Between June, 2006 and November, 2008, 33 cases of small chin patients underwent chin augmentation via Royamid(R) and Radiesse(R) injections, 9 and 24 cases respectively. No complications were observed through the follow up period. Twenty six patients showed satisfaction with aesthetic results at 6 months follow up. In 7 cases, temporary swelling and erythema were observed. The augmentation effect lasted more than 6 momths in both Royamid(R) hard and Radiesse(R) groups. In 7 cases of Royamid(R) hard group, the aesthetic effects lasted more than one year. Long lasting tissue filler seems to be a good alternative treatment modality for bone augmentation surgery if approached with strict protocol indications and careful patient selection.

A Trial of Radiesse(R) for Facial Soft Tissue Augmentation

Ideal charateristics of soft tissue fillers include longevity, biocompatibility, low adverse event profile and a reasonable cost. Radiesse(R) have many advantage as the desirable properties of facial filler. Authors introduce Radiesse(R) was recently introduced as a facial filler agent. Radiesse(R) was injected to 81 male and female patients in the study, using 27G fine needle. The age range of the patients was 22 to 48 years. The injected areas were nasal root, tip, nasolabial folds and jaw. We measured patient satisfaction scale during the period of follow up 2 to 6 months. The scale of satisfaction was good to excellent in 69 cases of 81 cases and fair in 9 cases of 81 cases. Patients satisfaction was poor in only 3 cases. Patients were observed no systemic effects but minimal adverse events which are leakage, nodule and subclinical inflammation. Radiesse(R) was highly effective, long lasting material for facial soft tissue augmentation. If a precise injection technique were applied, Radiesse(R) can be a very good option for real volumetric facial soft tissue augmentation.

The study on constructing tissue-engineered bone by seeding rat marrow stromal stem cells onto three-dimensional porous nano-hydroxylapatite / 医学研究生学报

Objective:To investigate the feasibility of using marrow stromal cells (MSC) as seed cells and three-dimensional porous nano-hydroxylapatite as scaffolds for constructing tissue-engineered bone.Methods:MSC from rat were cultured and induced to differentiate into osteoblasts in vitro. And then these induced cells were identified and seeded onto three-dimensional porous nano-hydroxylapatite scaffolds, cultured for 15 days in vitro. Scanning electron microscopy was used to evaluate the growth of these induced cells on three-dimensional porous nano-hydroxylapatite scaffolds. Results: The activity of alkaline phosphatase (ALP) and the secretion of osteocalcin of MSC from rat appeared and were increased gradually along the culture. The cells seeded on three-dimensional porous nano-hydroxylapatite scaffolds could adhere and proliferate well, and come into being many tiny calcium nodules and collagenous fibers. Conclusion:The data demonstrated that the new developed culture method is conducive to MSC’s differentiating and proliferating into osteoblasts that have a fine activity and the three-dimensional porous nano-hydroxylapatite may be considered as a suitable scaffold for the seeded cells. Using marrow stromal cells as seed cells and three-dimensional porous nano-hydroxylapatite as scaffolds is advantageous for constructing tissue-engineered bone.

Morphology of elderly people's periodontal ligament cells attached to the three-dimensional porous nano-hydroxylapatite / 医学研究生学报

Objective:Because of the lower proliferation ability of periodontal ligament cells of elderly people,how to choose suitable scaffold materials for them becomes very important.The aim of this study was to observe the morphological manifestation of the periodontal ligament cells of elderly people seeded onto the three-dimensional porous nano-hydroxylapatite scaffolds.Methods: Periodontal ligament cells from elderly people were seeded onto the three-dimensional porous nano-hydroxylapatite scaffolds,cultured for 15 days in vitro,and observed for the morphological changes by scanning electron microscopy in comparison with those of young people.Results:Fifteen days after seeded on the three-dimensional porous nano-hydroxylapatite scaffolds,the periodontal ligament cells of the elderly people exhibited sufficient adhesive behavior,proliferated well and produced tiny calcium nodules,but the number of the nodules was smaller than that in the young people.Conclusion: The periodontal ligament cells of elderly people grow well on three-dimensional porous nano-hydroxylapatite scaffolds,and can be considered as a suitable scaffold for periodontal tissue engineering in elderly people.

A Clinical Study of the Ossiculoplasty in the Chronic Otitis Media / 대한이비인후과학회지

Reconstruction of the middle ear conduction mechanism continues to pose a significant challenge to the otolaryngologist. To investigate the suitable material and surgical method to get optimal hearing gain in the middle ear surgery, two hundred and twenty-two cases of different kind of ossiculoplasty in patients suffering from chronic middle ear disease at the Severance hospital and Soh's ENT clinic from January 1989 to December 1994 were included in this retrospective study and we reviewed the previous reports about the ossiculoplasty at the department of otolaryngology, Yonsei University College of Medicine from January 1981 to December 1988. A successful hearing gain was defined as a post-operative air-bone gap of

A Clinical Study of the Ossiculoplasty in the Chronic Otitis Media with Cholesteatoma / 대한이비인후과학회지

The purpose of this study is to investigate the middle ear condition, surgical method and suitable material for optimal hearing gain in the middle ear cholesteatoma surgery. One thousand and thirty six cases of ossiculoplasty in patients suffering from chronic otitis media with cholesteatoma at the university hospitals in Korea from November 1984 to July 1996 were included in this retrospective study. Sixty two per cent of cases were successful according to the success criterion of a post-operative air-bone gap of >or= 30dB, 33.4 percent according to >or= 20dB, and 12.6 percent according to >or= 10dB. Good prognostic factors in the middle ear condition were healthy middle ear mucosa, patent eustachian tube orifice, and presence of superstructure of stapes. Two staged ossiculoplasty showed better result than one staged ossiculoplasty. There was no statistical difference of the hearing improvement between the open cavity mastoidectomy and the intact canal wall mastoidectomy techniques. Favorable materials for ossicular reconstruction were in decending order, polycel, ossicle, cartilage, and hydroxylapatite.

Cranioplasty Using Hydroxylapatite

Repair of skull defects for brain protection and aesthetics is still a surgical problem for which there is no completely satisfactory solution. Material used for cranioplasty should ideally be incorporated into the surrounding skull bone with a blood supply. Hydroxylapitite is a dense pure ceramic material which is biocompatible, non-toxic, non-inflammatory, non-resorptive and osteoconductive. The authors had experienced good cosmetic and functional results of cranioplasty Hydroxylapatite during 19 months of follow up period.

A preliminary Investigation of 25 Cases of Saddle Nose Anaplasty with Hydroxylapatite Ceramics / 昆明医科大学学报

25 cases of saddle nose anaplasty with hydroxylaptite ceramics have been reported in this paper.Anaesthesia and the operating methods have been modified to make the operating ef- fects more satisfactory.All of the merits of the modification have been discussed in the paper.

RESEARCH OF BONE REGENERATION WITH CULTURED OSTEOBLASTS SEEDED IN POROUS CORAL-HYDROXYLAPATITE / 解放军医学杂志

Rabbit osteoblasts were isolated, expanded in vitro, and then seeded into coral-hydroxylapatite. The osteoblasts/coral-hydroxylapatite composite was were implanted into the muscles of rabbits′ back. Bone regeneration was observed 4, 8 weeks after implantation by histological and scanning electron microscopic examinations.The results showed that osteoblasts could adhere and proliferate well on the surface of coral-hydroxylapatite. 4 weeks after implantation osteoid tissue was observed, 8 weeks after implantation large amount of new bone was found. It suggested that osteoblasts/coral-hydroxylapatite composite may be used for bone tissue engineering.