RESUMO
Resumen Escherichia coli 0157:H7 es una bacteria patógena reconocida por su capacidad de resistencia a diversos antibióticos; razón por la cual, se generan complicaciones en el tratamiento de infecciones producidas por esta bacteria. El péptido Ib-M1 y el bioconjugado I0NP@Ib-M1 han surgido como una nueva alternativa antimicrobiana contra E. coli 0157:H7. El mecanismo de acción de Ib-Mi e I0NP@Ib-M1 contra esta bacteria aún es desconocido; por lo tanto, el objetivo de esta investigación fue identificar el cambio en el perfil de proteínas de E. coli 0157:H7 luego del tratamiento con Ib-M1 e I0NP@ Ib-M1 como primer paso para determinar su mecanismo de acción. Para esto, se llevó a cabo la obtención de proteínas, posteriormente se realizó una electroforesis bidimensional para finalmente realizar la determinación de la variabilidad de los perfiles proteicos. Una vez obtenidos estos perfiles, se llevó a cabo un análisis de varianza (AN0VA). Se identificaron 72 proteínas expresadas diferencialmente, las cuales pueden relacionarse con el efecto sobre el crecimiento de la bacteria en presencia de Ib-M1 e I0NP@Ib-M. Estas proteínas se encuentran involucradas en procesos de transferencia de grupos acilo (proteína Yhbs), translocación de lipoproteínas (proteína LolA) y transporte de aminoácidos (proteína GpmA), entre otros.
Abstract Escherichia coli 0157: H7 is a pathogenic bacterium which is recognized for the ability to resist multiple antibiotics; accordingly, complications occur in the treatment of infections caused by this bacterium. The Ib-M1 peptide and the I0NP @ Ib-M1 bioconjugate have emerged as a new antimicrobial alternatives against E. coli 0157: H7. The mechanism of action of Ib-M1 and I0NP @ Ib-M1 against this bacterium is still unknown; therefore, the goal of this research was to identify the change in the proteins profile of E. coli 0157: H7 after treatment with Ib-M1 and I0NP @ Ib-M1 as a first step to determine its mechanism of action. For this, the proteins were obtained first, and then a two-dimensional electrophoresis was performed to finally determine the variability of the protein profiles. 0nce the protein profiles were obtained, an analysis of variance (AN0VA) was carried out. 72 differentially expressed proteins were identified, which can be connected to the effect on the bacterium's growth in the presence of Ib-M1 and I0NP @ Ib-M. These proteins are involved in acyl groups transfer processes (Yhbs protein), lipoprotein translocation (LolA protein) and amino acid transport (GpmA protein), among others.
Resumo Escherichia coli O157: H7 é uma bactéria patogênica reconhecida por sua capacidade de resistir a vários antibióticos; razão pela qual, complicações são geradas no tratamento de infecções produzidas por essa bactéria. O peptídeo Ib-M1 livre e imobilizado em nanopartículas magnéticas de óxido de ferro (IONP @ Ib-M1) surgiu como uma nova alternativa antimicrobiana contra E. coli O157: H7 e isolados clínicos desta bactéria. O mecanismo de ação de Ib-M1 e IONP @ Ib-M1 contra E. coli O157: H7 ainda é desconhecido; Portanto, o objetivo desta pesquisa foi identificar a alteração no perfil proteico de E. coli O157: H7 após o tratamento com Ib-M1 e IONP @ Ib-M1 como um primeiro passo para determinar seu mecanismo de ação. Para isso, foi realizada a obtenção das proteínas, posteriormente foi realizada uma eletroforese bidimensional para finalmente determinar a variabilidade dos perfis protéicos. Uma vez obtidos os perfis de proteínas, foi realizada uma análise de variância (ANOVA). Os resultados mostram a identificação de proteínas expressas diferencialmente e que estão envolvidas em processos de transferência de grupos acila (proteína Yhbs), translocação de lipoproteínas (proteína LolA) e transporte de aminoácidos (proteína GpmA), entre outros.
RESUMO
El presente estudio trata sobre una revisión documental relacionada con la predictibilidad en el diagnóstico utilizando Watson de IBM, el cual tiene como objetivo principal conocer el nivel de predictibilidad en el diagnóstico con Watson de IMB se ha utilizado un diseño no experimental de corte cualitativo donde las técnicas de recolección de datos han sido la revisión documental y el análisis de datos provenientes de estudios realizados por expertos que validan el uso de este tipo de tecnología, de acuerdo a los documentos consultados y en función de la opinión de los expertos se puede referir que la hipótesis planteada "el conocer el nivel de predictibilidad en el diagnóstico utilizando Watson de IMB contribuye a su implementación en el diagnóstico de enfermedades de orden oncológico" se cumple y como parte de algunas de las conclusiones las cuales refiere que el uso de este tipo de tecnología aporta a la prontitud en la generación de diagnósticos más certeros y con un nivel de predictibilidad media alta, al igual que es una oportunidad para el trabajo colaborativo entre profesionales de la salud y representa la necesidad imperante de actualizar sus conocimientos para proporcionar datos de calidad que faciliten una mayor predictibilidad del diagnóstico
The present study deals with a documentary review related to the predictability in the diagnosis using IBM Watson, which has as its main objective to know the level of predictability in the diagnosis with IBM Watson. a qualitative non-experimental design has been used where the data collection techniques have been the documentary review and the analysis of data from studies conducted by experts who validate the use of this type of technology, according to the documents consulted and based on the opinion of the experts, it can be stated that the hypothesis knowing the level of predictability in the diagnosis with Watson IMB contributes to its implementation in the diagnosis of oncological diseases is fulfilled and as part of some of the conclusions it is stated that the use of this type of technology contributes to the promptness in the generation of more accurate diagnoses and with a medium-high level of predictability, It is also an opportunity for collaborative work among health professionals and represents the imperative need to update their knowledge in order to provide quality data that facilitate greater diagnostic predictability
O presente estudo trata de uma revisão documental relacionada à previsibilidade no diagnóstico com o Watson da IBM, cujo objetivo principal é conhecer o nível de previsibilidade no diagnóstico com o Watson do IMB. Utilizou-se um desenho qualitativo não experimental onde foi utilizado. as técnicas de coleta de dados foram a revisão documental e a análise de dados de estudos realizados por especialistas que validam o uso desse tipo de tecnologia, de acordo com os documentos consultados e com base na opinião dos especialistas, pode-se referir que o hipótese levantada conhecer o nível de previsibilidade no diagnóstico com o Watson do IMB contribui para sua implementação no diagnóstico de doenças oncológicas é atendida e faz parte de algumas das conclusões que referem que o uso deste tipo de tecnologia contribui para a prontidão na geração de diagnósticos mais precisos e com um nível de pré ditabilidade média-alta, pois é uma oportunidade de trabalho colaborativo entre os profissionais de saúde e representa a necessidade predominante de atualização de conhecimentos para fornecer dados de qualidade que possibilitem maior previsibilidade do diagnóstico.
Assuntos
Revisão , Doença , OncologiaRESUMO
Introduction: Internet is one of the fastest growing media for Human Resources and Development (HRD). Computer has become an essential part of our Activities of Daily Livings (ADLs) at one hand and other hand, prolonged computer usage has increases the risk of occupational hazards. Prolonged Static work at computer system is a causative factor for poor postural ergonomic, structural derangements and dysfunction commonly at neck, shoulder and low back structures to develop functional limitation and progressively functional disabilities. The basic health education (HE) by means of ergonomic advises and exercise therapy may have efficacy to promote, prevent and cure for such FDs. However, internet itself can be a mode of HE system to be provided at workplace to cut the time & cost together. Purpose of Study: To find the efficacy of IBM for FDs of the computer users. Materials: Internet supported computer, web-site (www.ptmovements.com), web pages, basic health assessment form, Self Reported FD Questioners, Neck Disability Index (NDI) & Back Pain Functional Scale (BPFS), Study Design: Experimental Clinical Trial Methodology: 1256 computer users (M=867) participated online and assess for inclusive & exclusive criterions. Internet based self reported FDs questioners used to assess FDs in prior and post to two weeks of tailor made treatment program. Data Analysis: significance of FDs and impact of IBM on FDs was analyzed with SPSS -17, LOS set at 0.05 or CI 95 % Result: mild to moderate prevalence of FDs was higher neck & shoulder as compared to low back structures. The IBM has shown efficiency to reduce the level of FDs at NDI, and BPFS. Discussion: The internet based health education (IBHE) is well possible to deliver and to reduce the FDs. The advantage of time & cost effective approach in IBM has facilitated keen interest among computer users. Conclusion: The IBHE is well efficient and prospective to develop scopes of “workplace wellness” to promote, prevents, and cures for occupational diseases & disorders. E-health education has great prospectus in field of modern medicine.
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Sporadic inclusion body myositis (SIBM) is the most common idiopathic inflammatory myopathy in Caucasians over the age of 50 years. The prevalence of SIBM in the Asian population was initially thought to be very low, although the recent study showed that the prevalence of SIBM in Japan is in fact similar to the prevalence in Australia and the USA. SIBM is a refractory myositis with associated myodegenerative features mimicking the neuropathology of Alzheimer’s disease. The diagnosis of definite SIBM requires the typical clinical features and the presence of an autoaggressive inflammatory reaction, rimmed vacuoles, and congophilic deposits or tubulofilamentous inclusions. About one-fourth of patients with the typical clinical features of SIBM did not fulfill the pathological criteria of the definite SIBM. These canonical biopsy features may be absent in the early stage of the disease. Here we review the typical findings and the diagnostic pitfalls of the muscle biopsy in SIBM.