RESUMO
RESUMEN A finales del año 2019 el mundo conoció de la existencia y propagación de un nuevo coronavirus denominado SARS-CoV-2, capaz de provocar la enfermedad COVID-19. Las autoridades gubernamentales y de salud cubanas trazaron desde el principio estrategias de control epidemiológico, y fue el diagnóstico molecular por PCR en tiempo real una tarea de suma importancia para el control de la enfermedad en nuestro país. Un gran número de jóvenes profesionales y estudiantes de la Facultad de Biología de la Universidad de La Habana se sumaron a esta tarea. El presente trabajo aborda las principales actividades desarrolladas por estos últimos durante el diagnóstico molecular del SARS-CoV-2 en el Instituto de Medicina Tropical Pedro Kourí (IPK) en los primeros meses de la pandemia en nuestro país. El ejercicio de la profesión a partir de la puesta en práctica de habilidades y conocimientos teórico-prácticos, la adquisición de nuevos conocimientos, así como el fomento de valores éticos y morales como la solidaridad, el compañerismo y el trabajo mancomunado en colectivo, caracterizaron esta experiencia llena de desafíos y logros.
ABSTRACT At the end of 2019, the existence and spread of a novel coronavirus called SARS-CoV-2, responsible of the disease COVID-19 was known worldwide. From the beginning, the Cuban governmental and health authorities drawn up epidemiological control strategies, in which the molecular diagnosis by real-time PCR was of paramount importance for the control of the disease in our country. A large number of young professionals and students from the School of Biology of the University of Havana joined this task. This paper deals with the main activities performed by the students related to the molecular diagnosis of SARS-CoV-2 at the "Pedro Kourí" Institute of Tropical Medicine (IPK) in the first months of the pandemic in our country. The exercise of the profession in the implementation of the skills, and theoretical and practical knowledge; the acquisition of new knowledge; and the promotion of ethical and moral values such as solidarity, companionship, and joint work characterized this experience full of challenges and achievements.
Assuntos
Humanos , Adulto Jovem , Universidades , CubaRESUMO
El Instituto de Medicina Tropical Pedro Kouri (IPK) es una institución con un alto nivel científico, que desarrolla una formación docente de excelencia en doctorados, maestrías, residencias y otros entrenamientos relacionados con las enfermedades tropicales, su diagnóstico y tratamiento. La biblioteca, referencia en esta rama de la medicina, posee un fondo documental que se encuentra desactualizado, los documentos que forman parte de su colección son ediciones antiguas. Se hace necesario establecer una estrategia para la actualización y gestión de la información. Se realizó una búsqueda de información en internet para tener una visión sobre las herramientas y software utilizados para el desarrollo de bibliotecas digitales. Entre las herramientas consultadas y probadas se seleccionó el gestor de biblioteca de libros electrónicos Calibre por ser el software libre que más se adecuaba a nuestras necesidades. Se gestionó, reajustó y organizó la literatura en formato digital con el objetivo de establecer una estrategia para la actualización de la información, con la finalidad de satisfacer las necesidades de los usuarios(AU)
The IPK is an institution with a high scientific level, which develops teaching of excellence in doctorates, masters, residences and other trainings related to tropical diseases, their diagnosis and treatment. The library, a reference in this branch of medicine, has a documentary collection that is outdated, the documents that are part of its collection are old editions. It is necessary to establish a strategy for updating and managing information. An information search was carried out on the internet to have a vision on the tools and software used for the development of digital libraries. Among the tools consulted and tested, the Caliber e-book library manager was selected as the free software that best suited our needs. The literature was managed, readjusted and organized in digital format with the aim of establishing a strategy for updating information, in order to meet the needs of users(AU)
Assuntos
Humanos , Design de Software , Software , Doenças Transmissíveis/diagnóstico , Doenças Transmissíveis/tratamento farmacológico , Automação de Bibliotecas/normas , Serviços de Biblioteca , Estudos ProspectivosRESUMO
Background The molecular biological mechanisms of diabetic retinopathy(DR)is unclear up to now.Researches have proved that endoplasmic reticulum stress(ER Stress)-associated factors are elevated in peripheral blood in patients with diabetic retinopathy.and P58 IPK can inhibit those factors.So the relationship between P58 IPK and DR is worth to investigate. Objective The aim of this study was to detect the dynamic expression of P58 IPK in the retina of diabetic rats. Methods The diabetic animal models were established in 18 clean male SD rats by intraperitoneal injection of stilptozotiein(STZ)at a dose of 60 mg/kg.The rats were sacrificed in 1,3,6 months after injection.The expression change of P58 IPK mRNA in the rats retina was detected by quantitative real-time RT-PCR.Other 6 matched normal rats were used as control groups.This experiment followed the Regulations for the Administration of Affair Concerning experimental Animals by State Science and Technology Commission. Results The rats showed more drinking,more food and more urine after STZ injection with the blood glucose level≥ 16.5 mmol/L.The success rate of diabetic models was 100%.The A value of P58 IPK mRNA/β-actin in rat retina was 0.800±0.005 and 0.975±0.008 after injection of STZ.and that of control rats was 0.725±0.006,showing statistically significant difference between them(t=22.589,t=62.784,P＜0.05).In 6 months after injection of STZ,the expression of P58 IPK mRNA in experimental diabetic rat retina was evidently lower than the eontrol rats(0.671±0.004 versus 0.725±0.006,t=-17.984,P＜0.05).Conclusion P58 IPK has a close relation to the pathogenesis of DR,and it plays a retarding role for DR.