RESUMO
Background: To study the role of cell adhesion molecules like E-cadherin and ?-catenin expression in oral cancers in predicting tumor invasion and metastasis. The intercellular adhesions loss in epithelial malignancy is an important component of the acquisition of invasive and malignant properties. Material and Methods: All biopsies/ specimens were fixed in 10% neutral buffered formalin solution. Diagnosis was made on histopathology by using hematoxylin and eosin stains and further typing was done. Immunohistochemistry has been applied to see the expression of E-cadherin and ?-catenin in different grades of oral squamous cell carcinoma. Results: In this study, the assessment of E-cadherin and ?-catenin immunohistochemical stained slides were examined for pattern of staining (membranous, cytoplasmic or nuclear), proportion and intensity of staining of the tumor cells. The total score ranges between 0-12. Immunoreactivity was divided into three groups on the basis of final score: a total score of 0 is considered as negative immunoreactivity score, 1-4 score as low immunoreactivity score and >4 as high immunoreactivity score. Conclusion: In the present study, it was found that the reduced expression E-cadherin and ?-catenin is an indicator of a poor prognosis in oral squamous cell carcinoma.
RESUMO
Purpose: To enhance one's ability to diagnose intracranial microcystic meningioma. Methods: Fifteen cases of intracranial microcystic meningioma have been studied either clinicopathologically, or ultrastructurally or immunohistochemically. Results: The results indicate that electron microscopy and immunohistochemically are very helpful for the diagnosis of the tumor. There was no predilection as to location, however the tumor was more common on the base of skull. Vacuole-like structure and/or vesicular dilatation could be seen in the cytoplasm and capillaries could be observed in between the spindle cells by light microscopy. Under electron microscopy, the processes separated from each other and formed into a cystic structure, and bundles of collagenous fiber could be found in it. In immunohistochemistry, the stains with vimentin and epithelium membrane antigen (EMA) were positive. Conclusions: Intracranial microcystic meningioma has some characteristics under the microscope, immunohistochemistry is helpful in its diagnosis, and election microscopy can confirm this diagnosis.