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Objective Regenerating genes express mainly in gastrointestinal tissues and the injured regenerating pancreatic tissues,which can promote the regeneration of pancreatic β cells and other tissue cells.In recent years,researches on Reg family mainly involved the gene structure of various subtypes of Reg,and its role in diabetes,gastrointestinal cancer,inflammation,anti-microbial and the related mechanisms.Among the various subtypes of Reg,regenerating geneⅢ(Reg3) plays a particularly crucial role in these diseases.Therefore,Reg3 is a promising target for the treatment of these diseases.Based on the relationships of Reg3 with a variety of diseases,our group devote to the role of Reg3 [human REG3A,and mouse Reg3γ(Reg3g)] in type 1 diabetes,inflammation-linked pancreatic carcinogenesis,and the immunological changes participated in these processes.Hence,this review will summarize serial studies on Reg3 and the feasibility of it as drug targets.
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Post-transcriptional regulation of prokaryotes is of pivotal importance to the quality control and stability main -tenance of multiple proteins .The ubiquitin and ubiquitin-like proteins-mediated modifications have widely participated in the manipulation of cell cycle control , subcellular localization and cellular metabolisms .Typical ubiquitin like proteins (UBLs) are ubiquitin,SUMO,Nedd8 and FAT10.Recently, the dynamic regulation mechanisms between sumoylation and de-sumoylation have become the cutting edge in the era of ubiquitin-like modifications.In this review, we briefly summa-rized the entire SUMO-mediated modification systems and the intricate balance regulation .We also discussed the counter acting star molecule SENP1 and its relationship with tumor initiation and progression ,immunological regulation and develop-mental homeostasis .
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Objective: To compare the therapeutic effect of thymosin α1 combining anti-coagulation medication and simple anti-coagulation mediation in treating the patients with deep venous thrombosis (DVT) formation. Methods: A total of 92 patients with lower extremity vascular ultrasound conifrmed diagnosis of DVT were studied. The patients were randomly divided into 2 groups for treatment: Combination group, the patients received thymosin α1 combining anti-coagulation medication,n=45 and Simple group, the patients received only anti-coagulation medication,n=47. The changes of their deep venous thrombosis condition after treatment were compared between 2 groups. Results: By 4 weeks treatment, the proportion of completely dissolved thrombus in Combination group and in Simple group were 67 branches (49.26%) and 54 branches (37.24%); the thrombus progression and recurrence condition were 6 branches (4.41%) and 16 branches (11.03%), allP<0.05. Conclusion: Thymosin α1 combining anti-coagulation medication has the better effect than simple anti-coagulation medication for treating DVT patients.
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Objective: To investigate the effects of Liuwei Dihuang decoction (LW), its San-bu (three tonics) and Sanxie (three eliminators) ingredients on regulating the mmune function of senescence-accelerated mice (SAM). Methods: SAM-prone (SAMP) 8 mice were orally administrated with LW (10 g/kg), San-bu (6.4 g/kg) and San-xie (3.6 g/kg) respectively, once per day for 60 days, while SAM-resistant ( SAMR) 1 mice administrated with distilled water as control group. 3H-TdR incorporation was applied for detecting splenocyte proliferation. Flow cytometry (FCM) was used to observe the percentage of CD3+, CD4+,CD8+ and CD19+ ymphocytes in the spleens. Results: Compared with SAMR1 group, the splenocyte proliferation induced by ConA an LPS, percentages of CD3+, CD4 +, CD8+ and CD19+ lymphocytes and ratios of CD3+/CD19+ and CD4+/CD8+ in spleens decreased, while percentage of CD19+ lymphocytes increased in SAMP8 group. After administration of LW, San-bu and San-xie, the above ndexes were mproved to various degrees. LW showed better effects on improving LPS-induced splenocyte proliferation, percentages of CD3+, CD19+ and CD4+ lymphocytes, and the ratios of CD3+ /CD19+ and CD4+/CD8+ than San-bu and San-xie. San-bu showed better effect on mproving ConA-induced splenocyte proliferation than LW and San-xie, and better effect on mproving the percentages of CD3+, CD19+ and CD4+ lymphocytes in the spleens and the ratios of CD3+/CD19+ than San-xie, while San-xie had better effect on regulating CD4+/CD8+ ratio than San-bu. Conclusion: These results suggest that LW regulates the function of T and B lymphocytes and the mbalance of subsets of CD4+ and CD8+ lymphocytes in the spleens of SAMP8. LW shows better effects than San-bu or San-xie used alone, while San-bu and San-xie have their particular effects. The effects of LW on regulating the immune function might be the integral results of San-bu and San-xie. The results provid some experimental evidences for revealing the compatibility of LW.
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Besides the haemopoietic stem cell, mesenchymal stem cell (MSC) is an adult stem cell in the marrow. It has the ability to self-renew, differentiate into various kinds of tissue and regulate the immunological system. MSC possess low immunogenicity. It shows the immunosuppressive ability in vitro and has function of inducing immune-tolerance. MSC suppress accrementition of T cell respone to the allotigen and mitogen and hinderes the development of cytotoxic T lymphocyte cell. In addition, MSC can influence antigen presenting cell, natural kill cell, B cell, and so on.
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0.05);The rosettes looping rates of the2.5,5.0,10.0 mL/(kg?Bw)polypeptide groups were 33.3%,34.2%,39.6%higher than the control(injected with NS)(P
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Aim To study the antitumor and immunological regulation effects of ardipusilloside-Ⅰ(ADS-Ⅰ) on tumor-bearing mice of S180, H22, EAC and L1210. Methods The tumor-bearing mouse model was made through injecting cancer cells in the right limb oxter(or in abdominal cavity), and the mice were administrated orally with ADS-Ⅰfor 8 days. Then we detached the tumor and counted the inhibition rate of tumor growth. Incorporation of ()~3H-TdR was used to assess the proliferation of T spleen lymphocyte cells; phagocytosis of abdominal cavity macrophage was measured with the method of chicken red blood cells. Results ADS-Ⅰ was proved to be effective on the tumor-bearing mice of S180, H22 and EAC, and the maximal inhibition rate of the dose of 100 mg?kg~(-1) was 38.7%, 48.6% and 50.0%, respectively;ADS-Ⅰ promoted the proliferation of T spleen lymphocyte cells,and strengthened the phagocytosis of macrophage in the tumor-bearing mice significantly. Conclusion ADS-Ⅰ can inhibit the growth of transplanted tumor,and improve the immunological function of tumor-bearing mice.