Screening for potential biomarkers of traditional Chinese medicinechest impediment syndromesbased on plasma metabonomics / 中国药理学与毒理学杂志

OBJECTIVE To have a systematic pathomechanism view of three chest impediment-syndromes of Qi Deficiency and Blood Stasis syndrome(QDBS),Qi Stagnation and Blood Stasis syn-drome (QSBS), Cold Obstruction and Qi Stagnation syndrome(COQS) and further investigate the changed metabolome and related pathways for screening potential biomarkers in rat plasma. METHODS According to clinical pathogeny, three kinds of syndrome models were established to simulate the disease of chest impediment. Plasma metabonomics based on UPLC-Q-TOF/MS was applied in this research to detected small molecule metabolites for identifyingthe special potential biomarkers of three chest impediment syndromes, respectively. RESULTS Significant metabolic differences were observed between thecontrol group and three syndrome groups. Furthermore, three syndrome groups were distinguished clearly by pattern recognition method.The particular metabolites contributing most to the classification of three chest impediment syndromes were identified. In the QSBS group, the potential biomarkers could include 2-keto-glutaramic acid, L-methionine, L-homocysteic acid, octadecanamide, stearoylglycine,behenic acid,linoleylcarnitine,lysoPC(14:1(9Z)),indoxyl sulfate and cholic acid.In the COQS group, they could be aminoadipic acid, palmitic amide, oleamide, lysoPC(P-16:0), lysoPC(P-18:0), lysoPC(20:2(11Z,14Z)), 9-HETE and tauroursodeoxycholic acid. Moreover, 4-pyridoxic acid, L-palmi-toylcarnitine, lysoPC(20:0), lysoPC (22:5 (4Z,7Z,10Z,13Z,16Z)), 3- hydroxyhexadecanoic acid and arachidonic acid could be the potential biomarkers for the QDBS group. CONCLUSION Three chest impediment syndromes have their own potential biomarkers.Each special metabolite has its owndifferent metabolic pathway.Both metabolismof cysteine and methionine,and metabolism of alanine,aspartate and glutamate are the main pathways in regulation of metabolic disorders in QSBS syndrome. Lysine biosynthesis and degradation,fatty acid metabolism,and glycerophospholipid metabolism are the main pathways in regulation of metabolic disorders in COQS syndrome.Arachidonic acid metabolism, fatty acid metabolism,fatty acid elongation in mitochondria,and vitamin B6 metabolism are the main pathways in regulation of metabolic disorders in QDBS syndrome.These endogenous substances were indicated as the special potential biomarkers for three chest impediment syndromes and worth studying in depth.

Therapeutic Observation of Acupuncture plus Transcutaneous Electrical Acupoint Stimulation for Periarthritis of Shoulder due to Stagnation / 上海针灸杂志

Objective To observe the effect of acupuncture plus transcutaneous electrical acupoint stimulation (TEAS) on the range of motion and pain in stagnation-induced periarthritis of shoulder.Method A hundred patients with stagnation-induced periarthritis of shoulder were randomized into a treatment group and a control group, 50 cases each. The treatment group was intervened by acupuncture plus TEAS, while the control group was intervened by acupuncture alone. The interventions were given once every other day, 10 sessions as a treatment course, for 1 course in total. Before and after the intervention, the Visual Analogue Scale (VAS) was adopted for evaluating pain before and after the treatment, and the motor function scale of shoulder was used to measure the range of motion of shoulder joint. The clinical efficacies were also compared between the two groups.Result After the intervention, the VAS scores and shoulder motor function scores were significantly changed in both groups (P<0.05); the VAS score in the treatment group was significantly different from that in the control group after the treatment (P<0.05). The markedly effective rate was 74.0% in the treatment group versus 50.0% in the control group, and the between-group difference was statistically significant (P<0.05).Conclusion Acupuncture plus TEAS can produce a significant efficacy in treating periarthritis of shoulder, as it can obviously improve the range of motion and pain.

Literature Study on Acupuncture-moxibustion for Bi-impediment Syndrome in Ming and Qing Dynasties / 上海针灸杂志

Objective To summarize the treatment principle of acupuncture-moxibustion in treating Bi-impediment syndrome from the application rules of meridians and acupoints in Ming-Qing Dynasties by sorting out and analyzing the Chinese medicine literatures about acupuncture-moxibustion for Bi-impediment syndrome in Ming-Qing Dynasties, for providing literature evidence for basic and clinical research of Bi-impediment syndrome.Method Via electronic retrieval ofZhong Hua Yi Dian (Zhen Jiu Tui Na Lei) (Chinese Medical Encyclopedia,Chapter of Acupuncture-Moxibustion and Tuina), the data related to Bi-impediment syndrome in Ming-Qing Dynasties were extracted to establish a database categorized by meridians and acupoint features in Excel for analysis.Result There were 267 items of records about acupuncture-moxibustion in treating Bi-impediment syndrome in Ming-Qing Dynasties, involving the fourteen ordinary meridians, and 131 acupoints including 5 extra points; the frequency of using the Gallbladder Meridian ranked the top, followed by the Large Intestine Meridian; points from the Bladder Meridian were predominant, followed by the Gallbladder Meridian; there were 28 commonly-used acupoints (frequency>5), which were Quchi (LI 11, 26 times), Huantiao (GB 30, 23 times), Hegu (LI 4, 22 times), Chize (LU 5, 16 times),Yanglingquan (GB 34, 15 times), and Weizhong (BL 40, 14 times). Of the specific acupoint, the five Shu points were most frequently used, with a frequency of 217.Conclusion In the treatment of Bi-impediment syndrome with acupuncture-moxibustion, doctors in Ming and Qing Dynasties selected yang meridians more often than yin meridians, and Gallbladder, Large Intestine and Bladder Meridians had comparatively higher frequencies; regarding the application of acupoints, the specific acupoints were often used, especially the five Shu acupoints. The study results provide reference for acupoint selection in the treatment of Bi-impediment syndrome with acupuncture-moxibustion.

Literature Study on Acupuncture-moxibustion for Bi-impediment Syndrome in Ming and Qing Dynasties / 上海针灸杂志

Objective To summarize the treatment principle of acupuncture-moxibustion in treating Bi-impediment syndrome from the application rules of meridians and acupoints in Ming-Qing Dynasties by sorting out and analyzing the Chinese medicine literatures about acupuncture-moxibustion for Bi-impediment syndrome in Ming-Qing Dynasties, for providing literature evidence for basic and clinical research of Bi-impediment syndrome.Method Via electronic retrieval ofZhong Hua Yi Dian (Zhen Jiu Tui Na Lei) (Chinese Medical Encyclopedia,Chapter of Acupuncture-Moxibustion and Tuina), the data related to Bi-impediment syndrome in Ming-Qing Dynasties were extracted to establish a database categorized by meridians and acupoint features in Excel for analysis.Result There were 267 items of records about acupuncture-moxibustion in treating Bi-impediment syndrome in Ming-Qing Dynasties, involving the fourteen ordinary meridians, and 131 acupoints including 5 extra points; the frequency of using the Gallbladder Meridian ranked the top, followed by the Large Intestine Meridian; points from the Bladder Meridian were predominant, followed by the Gallbladder Meridian; there were 28 commonly-used acupoints (frequency>5), which were Quchi (LI 11, 26 times), Huantiao (GB 30, 23 times), Hegu (LI 4, 22 times), Chize (LU 5, 16 times),Yanglingquan (GB 34, 15 times), and Weizhong (BL 40, 14 times). Of the specific acupoint, the five Shu points were most frequently used, with a frequency of 217.Conclusion In the treatment of Bi-impediment syndrome with acupuncture-moxibustion, doctors in Ming and Qing Dynasties selected yang meridians more often than yin meridians, and Gallbladder, Large Intestine and Bladder Meridians had comparatively higher frequencies; regarding the application of acupoints, the specific acupoints were often used, especially the five Shu acupoints. The study results provide reference for acupoint selection in the treatment of Bi-impediment syndrome with acupuncture-moxibustion.

Analysis on Menghe physician Ma Peizhi's medication rule in prescriptions for impediment syndrome based on knowledge-discovery in database / 国际中医中药杂志

Objeetive To analyze the experiences for impediment syndrome of Ma Peizhi of Menghe Medical Genre by using traditional Chinese medicine (TCM) inheritance support system.Method The prescriptions for impediment syndrome of Ma Peizhi were collected and inputted to TCM inheritance support system,from which we can get the frequency of drug usage and the relationship between drugs based on the association rules and clustering algorithm.Results In the 61 prescriptions,the drugs that used most frequently were Chinese Angelica,Largeleaf Gentian Root,and Twotoothed Aehyranthes Root,and the drug combinations that used most frequently were Largeleaf Gentian Root-Chinese Angelica,Chinese Angelica-Largeleaf Gentian Root,Chinese Angelica-Mulberry Twig.And there were also 26 core combinations and 13 new prescriptions mined from the database.Conclusion Ma Peizhi of Menghe Medical Genre was well experienced impediment syndrome by dispelling wind and removing dampness,and promoting blood circulation by removing blood stasis,from which we can make a conclusion that TCM inheritance support system can be used to analyze the doctors' clinical experiences.