RESUMO
Objective To investigate the effect of radix salviae milliorhize on hemodynamics of portal hypertensive rats.Methods 60 Wistar rat models with portal hypertension were induced by CCl_4.Among them 56 rats with portal hypertension were divided at random into 4 groups: radix salviae 3 d group,radix salviae 10 d group,radix salviae 15 d group,and control group.After administration,the blood flow of vena portae,superior mesenteric artery,superior mesenteric artery and pressure of vena portae,average arterial pressure were measured.(Results Compared) with 0.9% Natrii Chloridi control group,the blood flow of vena portae,superior mesenteric artery,superior mesenteric artery and pressure of vena portae,average artery pressure in radix salviae milliorrhize 3,10 and 15 d groups were decreased significantly(P0.05).Conclusion Radix salviae milliorhize can be employed for treating portal hypertension.Radix salviae milliorrhize can decrease the blood flow and portal pressure,moreover,the effect can be enhanced with the elongation of treatment time.
RESUMO
Objective To study the therapeutic mechanism of medication p erfusion by femoral artery for the femoral head necrosis induced by glucocortico id experimentally. Methods Forty eight of New Zealand rabbits were used for esta blishing the model of femoral head necrosis. The rabbits were injected 50 ? g/ kg LPS in 24h twice intravenously, and methy prednisolone intramuscularly thre e times. The animals were divided into three groups: arterial group which was pe rfused urokinase 30 thousands units and 2 ml red sage root by left femoral arter y; venous group which was perfused urokinase 30 thousands units and 2 ml red sa ge root by era marginal vein; control group which was perfused normal saline. Mi crovessels ink perfusion and pathological examination were adopted to observed t he changes of the femoral head.The ratio of empty lacuna, fatty embolism, thromb us, VEGF positive osteoblast, chondrocyte and blood vessel in the subchondral pl ate were measured by means of HE, sudanⅢ , PTAH and immunohistochemistry in dif ferent periods (1,2,3,4weeks) after the medication perfusion.Results The treatme nt of urokinase and methyl- prednisolone could reduce the ratio of empty lacuna , fatty embolism, thrombus and increase the ratio of VEGF positive osteoblast, c hondrocyte and blood vessel, but the effects of femoral medication perfusion wer e more efficiency and quickly than vein injection. Conclusion The mechanisms con sist in re passing the blocked vessel by dissolving fatty embolism and thrombu s, accelerating the vascular developing and ossification by promoting the exudat ion of VEGF. [