Effect of ISL1 over-expression on proliferation of HIT-T15 cells / 中华内分泌代谢杂志

The effect of insulin gene enhancer binding protein (ISL1) on proliferation of HIT-T15 cells was investigated.ISL1 significantly promoted cell proliferation.ISL1 also increased the advance of HIT-T15 cell phase significantly.The results showed that ISL1 promoted proliferation of HIT-T15 cells.

Construction of recombinant retroviral vector with rat Islet-1 gene / 吉林大学学报(医学版)

Objective To isolate the rat insulin gene enhancer binding protein 1(Islet-1)gene and construct plEGFP-C1-Islet-1 recombinant retroviral expression vector.Methods The cDNA encoding the rat Islet-1 gene was isolated by RT-PCR method,the cDNA was first cloned into PGET-1 TA vector to facilitate the sequence and then subcloned into the retroviral vector plEGFP-C1.plEGFP-C1-Islet-1 was transfected into PA317 packaging cells with lipofectamine 2000.Transformants were selected in medium containing G418.Results A 1 050 bp DNA fragment was obtained by RT-PCR;plEGFP-C1-Islet-1 recombinant retroviral expression vector was identified by restrictive enzymes digestion,PA317 cells transfected with recombinant vector expressed enhancer green fluorescent protein(EGFP).Conclusion The gene encoding the rat Islet-1 is obtained and plEGFP-C1-Islet-1 expression vector is constructed successfully.