Forecasting of Molecular Sponge Mechanism Mediated by LOC389023 in Patients with Intractable Temporal Lobe Epilepsy / 中山大学学报(医学科学版)

[Objective]To forecast the sponge mechanism mediated by LOC389023 in patients with intractable temporal lobe epilepsy(TLE),through investigating the expression of microRNA interacted with dipeptidyl peptidase 10(DPP10)and LOC389023.[Methods]The expression of DPP10 and Kv4.3 were detected in 15 temporal neocortex from patients with brain trauma (control group)and in 26 temporal neocortex from patients with intractable TLE(epilepsy group)by western blot(WB)and immunohisto?chemical(IHC)staining. The location of DPP10 and voltage dependent potassium channel 4.3(Kv4.3)was detected by immunofluo?rescent(IF)staining. The interaction between DPP10 and Kv4.3 was testified by co-immunoprecipitation(Co-IP). The expression of microRNA obtained by softwares(miRanda,Pita,TargetScan and miRDB)was detected by qPCR.[Results]IHC and WB showed an increased expression of DPP10(P0.05). But decreased expression of LOC389023 and miR-140-5p and increased expression of miR-25-3p and miR-367-3p were found in epilepsygroup compared to control group (P < 0.05).[Conclusion]miR-25-3p and miR-367-3p may be regulated by LOC389023 through the sponge mechanism followed by altered expression of DPP10 in intractable temporal lobe epilepsy.

Identificação de regiões cerebrais de linguagem: estudo de ressonância magnética funcional em pacientes com epilepsia refratária de lobo temporal / Identifying language cerebral functions: a study of functional magnetic resonance imaging in patients with refractory temporal lobe epilepsy

OBJETIVO: Identificar as regiões cerebrais responsáveis pelas funções de linguagem através de Ressonância Magnética funcional (RMf) em pacientes com epilepsia refratária de lobo temporal. METODOLOGIA: Foi realizada RMf utilizando-se o paradigma de geração de verbos. Os pacientes ouviam palavras concretas e eram orientados a pensar na sua finalidade, sem verbalizar (ex: faca-cortar). Utilizou-se o cálculo de índice de lateralidade (IL) e a verificação do grau de ativação das áreas classicamente envolvidas na linguagem. RESULTADOS E CONCLUSÕES: Constatou-se que 92,3 por cento dos pacientes investigados apresentaram linguagem em hemisfério esquerdo, e 7,7 por cento, linguagem mista. Observou-se que existe maior freqüência de pacientes com ativação nas regiões de Broca esquerda, Córtex Pré-motor esquerdo, área de Wernicke esquerda e Córtex Auditivo esquerdo.

PURPOSE: The purpose of this study was to identify the brain regions for language functions with the functional Magnetic Resonance Image (fMRI) in patients suffering from intractable temporal lobe epilepsy. METHODS: During fMRI, patients performed a verb-generation task. They heard concrete words through headphones and were asked to think of their utility (e.g. pencil - to write), without verbalizing or making any facial or tongue movements, keeping silent and with eyes shut. A laterality index (LI) was calculated and we verify the brain regions activities for language. RESULTS AND CONCLUSIONS: Our results identified that that 92.3 percent of the patients analyzed by fMRI presented left hemisphere language and 7.7 percent presented mixed language representation. It was observed more patients with activation in left Broca area, left pré-motor cortex, left Wernicke area and left hearing cortex.

Surgical Outcomes in Children with Intractable Temporal Lobe Epilespy:Electroencephalographic and Pathologic Findings / 대한소아신경학회지

PURPOSE: Epilepsy surgery has become increasingly available in children with medically intractable epilepsy including temporal lobe epilepsy(TLE). TLE in children, however, has many different clinical and electrophysiologic characteristics which make presurgical evaluation difficult. The aim of this study is to evaluate the electroencephalographic(EEG) features which might be one of the predictors of postsurgical seizure outcomes in TLE. METHODS: Standard EEG, video-EEG, subdural or depth EEG were investigated in 12 children who underwent anterior temporal lobectomy, and their seizure semiology, MRI, and pathologic findings were also reviewed. Postsurgical seizure outcomes were divided into favorable(class I, II) and unfavorable(class III, IV) groups by using the Engel's classification. RESULTS: Half of the patients showed favorable outcomes, whose pathologic findings revealed hippocampal sclerosis or gliosis. Such pathologic findings were not demonstrated in MRI. Interictal epileptiform discharges were concordant with the location of the ictal onset in only 2 cases of the favorable outcomes. For the other 4 cases in the favorable group, focal ictal onset patterns were clearly evident in video-EEG monitorings or invasive EEG studies. Six cases in the unfavorable group showed unlocalized or multifocal interictal or ictal discharges -extratemporal, bitemporal or generalized epileptic activities- even in the invasive EEG studies. Five of them revealed cortical dysplasia in the pathology. CONCLUSION: The postsurgical seizure outcomes of hippocampal sclerosis or gliosis were more favorable than those of cortical dysplasia. Invasive EEG recordings should be considered for the localization of epileptic foci in the presurgical evaluation of children with intractable TLE.

Different Outcomes of Intractable Temporal Lobe Epilepsy with Regard to the Timing of Surgical Intervention / 대한소아신경학회지

PURPOSE: We like to examine the clinicopathologic findings of intractable temporal lobe epileptic patients who underwent epilepsy surgery, and different outcomes with regard to the timing of surgical intervention. METHODS: One hundred fourty six patients underwent anterior temporal lobectomy for medically intractable epilepsy between the year of 1993 and 2000. Except 5 patients who had malignancy, 132 patients were included in this study and were followed up for longer than 12 months after surgery. Two groups, under 15 years old(pediatric group) and over(adult group) according to the timing of surgery, were compared with clinical variables(seizure patterns, EEGs and brain MRI findings), pathologic findings and seizure outcomes. Seizure outcomes were divided as favorable in Class I and II or unfavorable in Class III and IV by using Engel's classification. RESULTS: Among 132 patients, 103 patients(78.0%) were classified as favorable and 29 patients(22.0%) unfavorable. Adult group had more favorable outcomes than pediatric group(82.1% vs 55.0%, P=0.007). Pathologic findings were as follows:48 patients(36.4%) had only hippocampal sclerosis, 50(37.9%) hippocampal sclerosis with other pathologic findings, 20(15.2%) cortical dysplasia, 6(4.5%) cortical dysplasia and gliosis, 7(5.3%) only gliosis and 1(0.7%) hippocampal atrophy. Among 98 patients who had hippocampal sclerosis, 81(82.7%) had favorable outcomes. Among 26 patients who had cortical dysplasia, 16(61.5%) had favorable outcomes. In case of hippocampal sclerosis only, pediatric group had more favorable outcomes(85.7% vs 82.9%, P=0.86). But in case of hippocampal sclerosis with other pathologic findings, adult group had more favorable outcomes(50.0% vs 86.4%). In case of cortical dysplasia only, adult group had more favorable outcomes(40.0 % vs 74.3%). CONCLUSION: The seizure outcomes after surgery, pediatric group showed less favorable outcomes than adult group. In case of hippocampal sclerosis only, the outcome of early surgery was good too.