RESUMO
Objective: To develop a RP-ion pair HPLC with wavelength switching for the simultaneous determination of 4 components (marine, oxymarine, salicylic acid, benzoic acid) in Fufang Kusheng Shuiyangsuan powder.Methods: An Agilent ZORBAX SB-C18 column(150 mm× 4.6 mm, 5 μm) was used;the mobile phase was acetonitrile (A)-0.1% phosphoric acid solution (0.2 g sodium heptanesulfonate was added to 100 ml solution) at a flow rate of 1.0 ml·min-1;the detection wavelength was 220 nm in 0-12 min and 280 nm in 12-25 min;the column temperature was 30℃.Results: The linear range of marine, oxymarine, salicylic acid and benzoic acid was 0.006 030-0.120 6 μg (r=0.999 4), 0.016 56-0.331 2 μg (r=0.999 9), 0.717 1-14.34 μg (r=0.999 9) and 0.512 0-10.24 μg (r=0.999 9), respectively;the average recovery was 98.14%, 97.20%, 97.05% and 98.39% with the RSDs of 1.38%, 0.32%, 0.81% and 1.26%(n=6) , respectively.Conclusion: The method is simple and rapid, and can be applied in the simultaneous determination of 4 components in Fufang Kusheng Shuiyangsuan powder.
RESUMO
Objective To establish a HPLC method for the determination of Hyperoside in Bushen-Yijing wan. Methods The HPLC system consisted of the Agilent HC-C18(4.6 mm×250 mm, 5μm)column was adopted. The mobile phase was consisted of acetonitrile(10)-HK2PO4(90)(0.85 g/L sodium heptanesulfonate,H3PO4 adjusted pH to 4.8), the flow rate was 1.0 ml/min, the column temperature was 30 ℃, and the UV detector was set at 360 nm. Results The linear response range was 3.0~60.0μg/ml(r=0.999 9). The average recovery of hyperoside was 98.2%, and RSD1.53%. Conclusions The method is simple, rapid, accurate and repeatable. It can be applied in determination of Hyperoside in Bushen-Yijing wan.
RESUMO
A rapid and simple chromatographic procedure using HPLC-ECD is described for simultaneousdetermination of NE. E. DA. 5-HT with their precursor amino acid (Tyr. Trp) and their main metabolites (HVA. 5-HIAA). Using this assay,eight substrates are measured in cortex, diencephalon and brain stem of mice duing immunological response challenged by SRB6 3 days after challenged, the DA HVA in cortex, NE in diencephalon decrease obviously (p