RESUMO
Objective To investigate the ontogeny of the islet amyloid polypeptide(IAPP)and explore the relationship IAPP and other classical hormones in human fetal colon and rectum. Methods The localization of IAPP immunoreactive(IR) cells and 5 hydroxytryptamine(5 HT) IR cells in 31 human fetal colon and rectum from 9\|27 weeks of gestation was investigated with immunohistochemical SABC method. Results At 9 weeks of fetal age,a lot of 5 HT IR cells were found in the colon,but IAPP IR cells appeared at 18 weeks.In the rectum,both 5 HT and IAPP IR cells were present at 11 weeks,the number of 5 HT IR cells ascended gradually with fetus aged,reached a peak at 20 weeks and dropped gradually after 21 weeks,while in the whole fetus period,IAPP IR cells appeared fewer and scattered.The co localization of IAPP and 5 HT in some cells of colon and rectum was proved as compared with adjacent sections.Immunohistochemical double staining procedures on the same tissue section also showed that IAPP co existed with 5 HT in some cells.Conclusion\ During the human fetus,IAPP were synthesized and co existed with 5 HT in endocrine cells of the colon and rectum.The possible function on the co existence of IAPP and 5 HT in the colon and rectum of fetal was discussed. [
RESUMO
Objective To observe the expression and localization of the islet amyloid polypeptide(IAPP) in the stomach of human fetus. Methods The localization of IAPP immunoreactive(IR) cells in 12 human fetal bodies of stomach from 11th to 20th week of gestation was investigated with immunohistochemical SABC method. Results At 11th week of human fetal age,the IAPP-IR cells had been found in the gastric mucosa.The IAPP-IR cells were dominantly concentrated in the fundic gland.The number of IAPP-IR cells increased gradually,and the immunostain of immunoreactive cells darkened gradually from 14th to 20th week of gestation.As compared with the adjacent sections which were stained by HE,some IAPP-IR cells were co-localization with parietal cells.Conclusion IAPP-IR cells exist in the human fetal body of stomach..