RESUMO
Objective To explore the role of the novel hydatidiform mole-related gene F10 in the tumorigenicity of choriocarcinoma cell line JAR in nude mice. Methods By cell transfection and RNA interference, stable F10 gene over-expression and F10 gene-silenced JAR choriocarcinoma cell line were engendered. Thirty SPF nude mice (aged 4-5 weeks) were randomly assigned into 3 groups (10 each): F10 overexpression group (inoculated with F10 gene-overexpressed JAR cells), control group (inoculated with non-treated JAR cells) and F10 gene-silenced group (inoculated with F10 gene-silenced JAR cells). JAR cell suspension (0.2ml with 5 x 107 cells in each mouse) were injected into the subcutaneous tissue of the back of mice neck. After injection, the conditions of the mice were observed, and they were weighed once every 3-4 days. The tumor formation time was recorded, the tumor growth curve was plotted, and tumor formation rate was calculated. The mice were sacrificed, and the subcutaneous tumor mass was weighed 5 weeks after the JAR cell injection. Results The rate of tumor formation was 100% (10/10). There was no significant difference in tumor formation time (F=0.097, P=0.908) among the groups of F10 overexpression (4.4 ± 1.1d), F10 silenced (4.4 ± 1.1d), and control (4.6 ± 1.3d). The growth rate of subcutaneous tumor in F10 overexpression group was significantly higher compared with groups of control and F10 silenced (P<0.05), and significant difference in tumor growth rate was found between control group and F10 silenced-expression group (P<0.05). The weight of tumor tissue was significantly different (F=14.462, P=0.000) among the groups of F10 overexpression (607.49 ± 216.19mg), F10 silenced (270.73 ± 81.53mg) and control (423.87 ± 74.75mg). Conclusion F10 gene is involved in the proliferation of JAR choriocarcinoma cell line, and it might enhance the tumorigenicity of JAR cell line in nude mice.