Establishment of UPLC fingerprints of Jasminum elongatum and determination of two constituents / 中成药

AIM To establish the UPLC fingerprints of Jasminum elongatum (Bergium) Wild.and to determine the contents of isochlorogenic acid B and ethylcaffeate.METHODS The analysis of methanol extract of J.elongatum was developed on a 25 ℃ thermostatic Agilent Ecplise XDB-C18 column (2.1 mm × 100 mm,1.7 μm),with the mobile phase comprising of acetonitrile-0.1％ methanoic acid flowing at 0.5 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.RESULTS There were eighteen common peaks in the fingerprints of ten batches of samples,with the similarities of more than 0.85.Isochlorogenic acid B and ethylcaffeate showed good linear relationships within the ranges of 7.67-38.35 μg/mL (R2 =0.999 4),9.60-96.0 μg/mL (R2 =0.999 7),whose average recoveries were 98.61％,99.09％ with the RSDs of 0.84％,1.25％,respectively.CONCLUSION This stable and reliable method can be used for the quality control of J.elongatum.

Identification of Ligustrum sinense Lour. And Jasminum elongatum (Bergium) Wild / 广州中医药大学学报

Objective To identify the Ligustrum sinense Lour.and Jasminum elongatum(Bergium) Wild.from the macroscopic appearance and microscopic features.Methods Fresh samples of stems,leaves,flowers and fruits of the two kinds of medicinal plants were harvested.Stereoscopy was used for the observation of macroscopic appearance of Ligustrum sinense Lour.and Jasminum elongatum (Bergium) Wild.,and the microscopy was used for the examination of their microscopic features of stem and leaf cross section,tear film and the powder of leaf upper and lower epidermis.Results Original plant characteristics of the two kinds of species were as follows:Ligustrum sinense Lour.had a racemes and funnel-shaped corolla,while Jasminum elongatum (Bergium)Wild.had a cymes and salverform corolla.Microscopic identification results were as follows:the stem pith of Ligustrum sinense Lour.was smaller,and the stem pith ofJasminum elongatum(Bergium) Wild.was bigger;Ligustrum sinense Lour.stem powder had less pit or texture in the sclereids,and had reticulate vessels,and Jasminum elongatum(Bergium) Wild.stem powder had apparent pit or texture in the sclereids,and had spiral vessels.Conclusion The results will provide a basis for the identification,exploitation and utilization of Ligustrum sinense Lour.and Jasminum elongatum(Bergium) Wild.