RESUMO
The neurovascular unit (NVU) is a dynamic functional module composed of neurons,microglia,blood-brain barrier (BBB) and the extracellular matrix that maintains the integrity of brain tissues.The chief members of NVU are one of the structural bases of the development of nervous system diseases.And they are involved in the pathological process of depression.The NVU instability of depression is characterized by the uncoupling of nerve and blood vessels,the increase of BBB permeability and the inflammatory response.Traditional Chinese medicine (TCM) has many advantages in the treatment of depression,and the effect of Kai-Xin Jie-Yu (KXJY) decoction in treating depression is remarkable.The purpose of this study was to explain the effect of KXJY decoction on NVU in terms of the functional connectivity of brain white matter,the protection of neurons and glial cells,the protection of vascular endothelial cells,the repair of BBB and the reduction of inflammation.It provided a new theoretical basis and method for the treatment of depression in TCM.
RESUMO
This study was aimed to detect the expression of miR-665 in the hippocampal of depression rat model treated with Kai-Xin Jie-Yu (KXJY) decoction and bioinformatic analysis of target genes of miR-665,in order to investigate the role of miR-665 in the pathogenesis of depression and the antidepressant mechanism of KXJY decoction.The rat model of chronic stress depression was established and then treated with KXJY decoction for 42 days.The total RNA from hippocampus tissues was extracted.And the relative expression of miR-665 in hippocampus of rats in each group was detected by RT-qPCR.TargetScan and microRNAorg databases were used to predict target genes for miR-665.DAVID database was used to classify GO function and to analyze KEGG signaling pathway of target genes.The results showed that compared with the normal group,the expression level of miR-665 in hippocampus of the model group was significantly higher with significant difference (P ＜ 0.01).Compared with the model group,the expression level of miR-665 in hippocampus of Chinese medicine group and western medicine group decreased significantly with significant difference (P ＜ 0.01).The biological functions of miR-665 target genes were mainly concentrated in the response to organic substance.The signal pathway was mainly concentrated in N-Glycan biosynthesis.It was concluded that miR-665 may be involved in the pathological process of depression,by correcting the abnormal expression of miR-665,which may be one of the antidepressant mechanisms of KXJY decoction.Through the analysis and prediction of the target genes,it provided a certain direction and theoretical basis for further study on the specific mechanism of KXJY decoction intervention on miR-665.