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OBJECTIVE To explore the material basis and potential mechanism of Kazakh classic prescription Wuzdekh （WZDK） in the treatment of enteritis. METHODS LC-MS/MS technology was used to analyze the chemical components in WZDK. Through network pharmacology and molecular docking technology， the main chemical components of WZDK were screened and the target was predicted； therapeutic effect and target of WZDK on acute enteritis were verified through in vivo experiments. The acute enteritis model of mice was induced by dextran sulfate sodium salt； the general condition of the mice was observed during administration and the disease activity index （DAI） score was calculated； pathological changes of the intestine and mRNA expression of core target were validated by HE staining and quantitative real-time PCR. RESULTS A total of 316 chemical components were obtained by LC-MS/MS. The core targets of network pharmacological analysis mainly included interleukin 1β（IL- 1β）， protein kinase B1 （AKT1）， tumor protein p53 （TP53）， IL-6， tumor necrosis factor （TNF） and so on. The results of molecular docking showed that chemical components such as mairin， lappadilactone， costunolide and dehydrocostus lactone were stable in binding to the core target. The results of in vivo experiment showed that， compared with model group， high dose （5.00 g/kg） of WZDK could significantly reduce the DAI score （P＜0.05）， improve inflammatory cell infiltration and mucosal tissue damage of colon tissue， and significantly down-regulated mRNA expressions of IL-6， TNF-α， IL-1β and TP53 in colon tissue（P＜ 0.05 or P＜0.01）. CONCLUSIONS Chemical components of WZDK such as mairin， lappadilactone， costunolide and dehydrocostus lactone may play the role of improving the imbalance of local inflammatory factors in the intestine and repairing damage of colonic mucosal tissue by down-regulating mRNA expressions of TNF-α， IL-6， IL-1β and TP53 in colon tissue.
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Objective To explore the research status quo, hotspots and developing trends of Kazakh medicine. Methods Relevant literature of Kazakh medicine published from January of 1990 to December of 2015 was retrieved from CNKI and Wanfang database. Bibliographic information data were set as the research objects for data cleaning and data conversion. Metrological analysis was conducted from publication number, publication units, key words, and authors. Ucinet, CiteSpace, SATI and R language were used as analysis tools to construct social network and shared network. Results After screening, 142 articles were included. Institute of cooperative social network standardization center point penetration potential was 8.475%; top 3 high-yield authors were Mulati Kiaibek (10 articles), Alhin?Dakai (8 articles) and Maldives Jiang (7 articles); author co-citation map had a total of 189 nodes, 141 lines, and density of 0.007 9; key words were six element theory, traditional therapy, Soerbuen and Qieketarbe. Conclusion Kazakh medicine research is in the initial stage. The distribution of Kazakh medicine research team is relatively concentrated, while the hotspots of research were scattered. Featured medical resources of Kazakh medicine is with large space for mining.
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Objective To explore the research status quo, hotspots and developing trends of Kazakh medicine. Methods Relevant literature of Kazakh medicine published from January of 1990 to December of 2015 was retrieved from CNKI and Wanfang database. Bibliographic information data were set as the research objects for data cleaning and data conversion. Metrological analysis was conducted from publication number, publication units, key words, and authors. Ucinet, CiteSpace, SATI and R language were used as analysis tools to construct social network and shared network. Results After screening, 142 articles were included. Institute of cooperative social network standardization center point penetration potential was 8.475%; top 3 high-yield authors were Mulati Kiaibek (10 articles), Alhin?Dakai (8 articles) and Maldives Jiang (7 articles); author co-citation map had a total of 189 nodes, 141 lines, and density of 0.007 9; key words were six element theory, traditional therapy, Soerbuen and Qieketarbe. Conclusion Kazakh medicine research is in the initial stage. The distribution of Kazakh medicine research team is relatively concentrated, while the hotspots of research were scattered. Featured medical resources of Kazakh medicine is with large space for mining.