SILENT BEAST AROUND YOU: AN OVERVIEW OF SERIAL KILLERS IN INDIA

The worldview that portrays a serial killer as being a white male an evil monster with unusual appearance having dysfunctional behaviours and relationship, engaging in animals torture or being sexually abused in childhood and therefore sadistically killing a targeted or specific population for sexual gratification should be challenged (Bearby-2004) Every serial killer drives to kill multiple victims which may be unique, dependent on his/her childhood history and experiences We try to find out commonalities and differences between them as route to identify possible life events and factors that converted a normal man into serial killers Following factors were identified Ÿ Stress and trauma Ÿ Need for belonging and loneliness Ÿ Power or Control Ÿ Low self-esteem Ÿ Sexually sadistic and violent pornography Ÿ Parents relationship pattern Ÿ Neurodevelopment complication

O crime como ato perverso: uma análise das cartas de assassinos seriais / El crimen como un acto perverso: un análisis de cartas de asesinos en serie / Crime as a perverse act: analysis of letters from serial killers

RESUMO. Este trabalho se insere no campo teórico da psicanálise e tem por objetivo discutir, a partir do conceito de perversão, a relação entre o ato perverso e os assassinatos em série, através das comunicações que certos autores desse tipo de crime realizaram com a mídia e com as forças da lei. Para a realização desse objetivo foi feita uma análise de conteúdo, segundo Bardin (2011), em 35 cartas enviadas por sete assassinos em série diferentes para a grande mídia ou para a polícia. Como resultado, encontra-se o fato de que a estrutura desses documentos é bastante similar e apresentam descrições de seus crimes, seus estados mentais, além de ameaças à população e um deboche direcionado às autoridades e forças policiais. Por fim, nota-se que a estrutura do ato perverso, conforme pensada por Freud e Lacan, está presente nas cartas estudadas, que pertencem a épocas e lugares distintos, e cujos autores não tiveram contato direto entre si.

RESUMEN. Este trabajo es parte del campo teórico del psicoanálisis y tiene como objetivo identificar los comportamientos comunes que están presentes en diferentes actos perversos, más específicamente en las comunicaciones que los asesinos en serie llevan a cabo con los medios de comunicación y las fuerzas de la ley. Para lograr este objetivo, se realizó un análisis de contenido, según Bardin (2011) sobre 35 cartas enviadas por siete asesinos en serie diferentes a los principales medios de comunicación o la policía. Como resultado, existe el hecho de que la estructura de estos documentos es bastante similar y presenta descripciones de sus crímenes, sus estados mentales, además de las amenazas a la población y un libertinaje dirigido a las autoridades y las fuerzas policiales. Finalmente, se observa que la estructura del acto perverso, como lo piensan las teorías de Freud y Lacan, tiende a repetirse en los sujetos estudiados, que pertenecen a diferentes tiempos y lugares y que no tuvieron contacto directo entre sí.

ABSTRACT This work is part of the theoretical field of psychoanalysis and aims to discuss, from the concept of perversion, the relationship between the perverse act and serial murders, through the communications that certain authors of this type of crime made with the media and with the forces of the law. To achieve this objective, a content analysis was carried out, according to Bardin (2011), on thirty-five letters sent by seven different serial killers to the mainstream media or the police. As a result, there is the fact that the structure of these documents is quite similar and presents descriptions of their crimes, their mental states, in addition to threats to the population and a debauchery directed at the authorities and police forces. Finally, it is noted that the structure of the perverse act, as thought by the theories of Freud and Lacan, is present in the studied letters, which belong to different times and places, and whose authors had no direct contact with each other.

La neurobiología del asesino: aspectos neuroanatómicos, genéticos, bioquímicos, extrínsecos y sociales / The neurobiology of the killer: neuroanatomical, genetic, biochemical, extrinsic and social aspects / A neurobiologia do assassino: aspectos neuroanatômicos, genéticos, bioquímicos, extrínsecos e sociais

El cuestionamiento del por qué alguien querría asesinar a otra persona aún no tiene una respuesta única: ¿supervivencia, odio, placer, venganza? Frente a este fenómeno, las neurociencias ofrecen un espacio de comprensión relevante y actual para dar respuesta a este interrogante. Diversos estudios clínicos y en neuroimagen han mostrado diversas alteraciones anatómicas, bioquímicas, genéticas y su relación con una variedad de redes neuronales asociadas a conductas agresivas. Subregiones en la corteza prefrontal, la amígdala, el hipocampo y el lóbulo temporal juegan un papel importante en el desarrollo y el funcionamiento de la biología de la agresión. Variaciones genéticas en la regulación de proteinas y neurotransmisores tales como la serotonina y la dopamina han sido establecidas como mediadores en las conductas agresivas y homicidas. Las interacciones epigenéticas y los mediadores sociales representan importantes factores de riesgo adicionales para la agresividad. El presente artículo sistematiza algunos de los factores que influyen en la conducta homicida. Delimita sus factores de riesgo y correlato neurobiológico, así como aporta información basada en evidencia que ayude en la prevención de dichos comportamientos, la comprensión multidimensional del delito y el desarrollo de intervenciones efectivas fundamentadas en las neurociencias cognitivas forenses.

The question of why someone would want to murder another person still has no single answer: survival, hatred, pleasure, revenge? In the face of this phenomenon, neuroscience offers a relevant and current area of understanding to answer this question. Several clinical and neuroimaging studies have shown diverse anatomical, biochemical and genetic alterations and their relationship with a variety of neural networks associated with aggressive behaviors. Subregions in the prefrontal cortex, amygdala, hippocampus and temporal lobe play an important role in the development and functioning of the biology of aggression. Genetic variations in the regulation of proteins and neurotransmitters such as serotonin and dopamine have been established as mediators of aggressive and homicidal behaviors. Epigenetic interactions and social mediators represent additional important risk factors for aggression. The present article systematizes some of the factors that influence homicidal behavior. It delineates their risk factors and neurobiological correlates, as well as provides evidence-based information to aid in the prevention of such behaviors, the multidimensional understanding of crime, and the development of effective interventions based on forensic cognitive neuroscience.

A questão de pôr que alguém quereria matar outra pessoa ainda não tem uma única resposta: sobrevivência, ódio, prazer, vingança? Diante deste fenômeno, as neurociências oferecem uma área relevante e atual de compreensão para responder a esta pergunta. Vários estudos clínicos e de neuroimagem têm mostrado diversas alterações anatômicas, bioquímicas e genéticas e sua relação com uma variedade de redes neurais associadas a comportamentos agressivos. Sub-regiões no córtex pré-frontal, amígdala, hipocampo e lobo temporal desempenham um papel importante no desenvolvimento e funcionamento da biologia da agressão. Variações genéticas na regulação de proteínas e neurotransmissores como a serotonina e a dopamina foram estabelecidas como mediadores de comportamentos agressivos e homicidas. As interações epigenéticas e os mediadores sociais representam fatores de risco adicionais importantes para a agressão. Este artigo sistematiza alguns dos fatores que influenciam o comportamento homicida. Ela delineia seus fatores de risco e correlatos neurobiológicos e fornece informações baseadas em evidências para ajudar na prevenção de tais comportamentos, na compreensão multidimensional do crime e no desenvolvimento de intervenções eficazes baseadas na neurociência cognitiva forense.

Diseño y optimización de un tubo policromático de citometría de flujo para inmunofenotipo linfocitario periférico / Design and optimization of a polychromatic tube of flow cytometry for peripheral lymphocyte immunophenotype

Introducción: La citometría de flujo permite la cuantificación de las subpoblaciones de linfocitos con una elevada sensibilidad, especificidad y objetividad. Estas ventajas solo se logran con un proceso laborioso de diseño individualizado y controlado para cada experimento. Objetivo: Diseñar un protocolo de un solo tubo policromático de citometría flujo para inmunofenotipo linfocitario periférico. Métodos: Se realizó un estudio experimental in vitro con muestras de sangre periférica obtenidas de tres voluntarios sanos, en el Centro Nacional de Genética Médica, en marzo de 2019. El tubo se compuso de seis marcadores de linaje para identificar linfocitos B, T, natural killer y natural killer T. Se desarrolló un protocolo de lisis de hematíes sin lavado. Se emplearon anticuerpos monoclonales conjugados con fluorocromos. El punto óptimo de concentración correspondió al mayor índice de tinción y conservación de los porcentajes de positividad de cada población. Se realizó la construcción progresiva del tubo y se propuso una estrategia lógica de secuencia de ventanas para el análisis de datos. Resultados: Los marcadores seleccionados permitieron realizar correctamente el inmunofenotipo linfocitario periférico. En los cinco puntos de titulación se observaron buenas discriminaciones entre las señales positivas y negativas, excepto para el anti-CD56 que presentó una tendencia decreciente del índice de tinción. El volumen total de conjugados requeridos para la determinación de los 6 antígenos fue de 3,75 μL por tubo. Conclusiones: Se obtuvo un tubo policromático que permite el inmunofenotipo periférico de forma rápida y precisa por seis antígenos linfocitarios simultáneamente, con el empleo de pequeños volúmenes de conjugado y sangre(AU)

Introduction: Flow cytometry allows quantification of lymphocyte subpopulations with high sensitivity, specificity and objectivity. These advantages are only achieved through the hardworking process of individualized and controlled design for each experiment. Objective: To design a flow cytometry protocol of a single polychromatic tube for peripheral lymphocyte immunophenotype. Methods: An experimental in vitro study was carried out, in March 2019, with peripheral blood samples obtained from three healthy volunteers, at the National Center for Medical Genetics. The tube was made up of six lineage markers for identifying natural B and T lymphocytes, natural killers and natural killer T cells. A protocol was developed for red blood cell lysis without washing. Fluorochrome-conjugated monoclonal antibodies were used. The optimal point of concentration corresponded to the highest staining index and preservation of the positivity percentages of each population. Progressive tube construction was performed and a logical window sequence strategy was proposed for data analysis. Results: The chosen markers allowed to carry out correct peripheral lymphocyte immunophenotype. Good discriminations between positive and negative signals were observed at the five titration points, except for anti-CD56, which presented a decreasing trend in the staining index. The total volume of conjugates required for determination of the six antigens was 3.75 μL per tube. Conclusions: A polychromatic tube was obtained that allows to carry out peripheral immunophenotype quickly and precisely by six lymphocyte antigens simultaneously, with the use of small volumes of conjugate and blood(AU)

Knowledge, Attitude & Practice of Self-Medication with Painkillers among Young Adults, Bangladesh

@#Self-medication, the unsolicited use of prescription and over the counter (OTC) drugs, is practised all over the world, particularly in countries like Bangladesh. So, this study is focused on the prevalence and attitude and knowledge about various aspects of self-medication with painkillers among students of the selected residential university of Dhaka, Bangladesh. Methods It is a cross-sectional interview-based study. The study population was the students of the university who lives in attached residential halls. The total number of respondents was 209. Results Every respondent both male (105) and female (104) have reported that they had practiced self-medication with painkillers for their both acute and chronic pain like headache, menstrual cramp, back/muscle/joint pain etc. Drugs commonly used by the students were Napa (19.6%), Paracetamol (12.9%), Etorix (6.2%), ACE (10.0%) HPR ds (10.0%), Rolac (9.6%) etc. Many of the drugs were non-OTC drugs. The main sources of information were advised by the manager of the pharmacy (36.8%), self-decision (16.7%), media (12.0%) and advice from friends/relatives (23.0%). The prime factors that influenced students to try self -medicated painkillers were dissatisfaction toward the medical centre in the area (23.9%), cost-effectiveness (20.6%), unavailability of medical facilities (11.5%) etc. 64 of the respondents (both M & F) reported that they have no clear idea about the possible side effects of the chronic use of painkillers. Dependency on a painkiller for pain management was (M=32 and F=48). Conclusions 41% of students taking a self-medicated painkiller with analgesics in an emergency perceive it as safe.

Linfoma Extranodal de Células T/NK en Cavidad Oral. Reporte de un Caso en Chile / Extranodal Lymphoma of T / NK Cells in Oral Cavity. Case Report in Chile

RESUMEN: El linfoma extranodal de células T/NK es una neoplasia maligna agresiva que se caracteriza por una destrucción de estructuras de la línea media de la cara como paladar y fosa nasal. Presentamos el caso de un paciente de sexo masculino, 48 años de edad, consumidor de cocaína, que consulta en la Facultad de Odontología de la Universidad de Chile en Septiembre del 2015 por síntomas de disfagia, rinorrea y que presenta al examen clínico un tumor ulcerado que compromete paladar duro y blando, de un mes de evolución. Se confirma diagnóstico de linfoma de células T/NK con una batería de pruebas inmunohistoquímicas. Esta patología, aunque infrecuente, siempre debe ser considerada dentro los diagnósticos diferenciales en tumores ulcerados en esta localización.

ABSTRACT: Extranodal T / NK cell lymphoma is an aggressive malignant neoplasm characterized by destruction of midline structures of the face such as the palate and nasal fossa. We present the case of a male patient, 48 years old, cocaine user, who consults at the Faculty of Dentistry of the Universidad de Chile in September of 2015 due to symptoms of dysphagia, rhinorrhea and presenting to the clinical examination an ulcerated tumor which compromises hard and soft palate, a month of evolution. Diagnosis of T / NK cell lymphoma is confirmed with a battery of immunohistochemical tests. This pathology, although infrequent, should always be considered within the differential diagnoses in ulcerated tumors of this location.

The relationship between DC-CIK and colorectal cancer prognoses and its influen-cing factors / 药学实践杂志

Objective To discuss dendritic cell-cytokine-induced killer (DC-CIK ) cell therapy effects and clinical out-comes in patients with colorectal cancer in order to have better clinical treatment .Methods A retrospective analysis of the data of 66 patients with colorectal cancer from the Biological Therapy Department of the Eastern Hepatobiliary Surgery Hospital was performed from January 2012 to January 2014 ,and then was followed up .Taking gender ,age ,degree of pathological differen-tiation ,TNM staging ,surgical methods ,and targeted therapy as the research basis ,by the Kaplan-Meier single factor and Cox multiple factors analysis we mainly discuss the DC-CIK cell treatment′s effect on the prognoses of patients .Results Kaplan-Meier single factor analysis results indicate :to a certain extent ,DC-CIK cell therapy can improve the prognoses of patients ;Cox multi-factor analysis results indicate whether accepting DC-CIK cell therapy is an independent factor influencing the prog-noses of patients .Conclusion DC-CIK cells therapy can improve the prognoses of patients with colorectal cancer .

Assassinos seriais e os efeitos da sideração no psiquismo e no laço social / Serial killers and the effects of utter consternation on the psyche and social bonds / Les assassins en série et les effets de la sidération dans le psychisme et lien social / Los asesinos en serie y los efectos del encantamiento en la psique y el lazo social

Os processos que caracterizam a constituição da subjetividade dos assassinos seriais relacionam-se ao papel que neles têm o superego arcaico. Ao analisarmos as interfaces destes tipos de configurações psíquicas com os laços sociais, nos deparamos com as figuras dos assassinos seriais tratados como ícones da cultura pós-moderna, uma vez que são idealizados em filmes, livros e séries de TV, além de serem cultuados por muitos por sua capacidade de encanto, inteligência e engodo, movimentos que sideram e compactuam com os laços sociais perversos observados na contemporaneidade.

The processes that characterize the constitution of subjectivity of serial killers have a relationship with the role played by the archaic super-ego. When we analyze the interfaces of these types of psychic configurations with social bonds we see serial killers treated as icons of the post-modern culture, to the extent that they are idealized in the cinema, books and television series. They are also idolized by many for their ability to mesmerize, deceive and defraud others. These characteristics leads persons to support and approve perverse social bonds present in contemporary society.

Les processus qui caractérisent la constitution de la subjectivité des assassins en série se rapportent au rôle que le Surmoi archaïque joue chez eux. En analysant les interfaces entre ces types de configuration psychique et les liens sociaux, nous observons que ces assassins en série sont traités comme icônes de la culture post-moderne, étant donné qu'ils sont idéalisés dans les films, les livres et dans les séries TV. Ils sont d'ailleurs admirés par un nombre important de personnes dû à leur intelligence, ainsi qu'à leur capacité d'envoûter et de leurrer leurs victimes, qualités qui sidèrent et pactisent avec les liens sociaux pervers de notre contemporanéité.

Los procesos psíquicos que caracterizan la constitución de la subjetividad de los asesinos seriales se relacionan con el papel que en ellos tiene el Superyó arcaico. El análisis de las interfaces de estes tipos de configuraciones psíquicas con los lazos sociales muestra que las figuras de los asesinos en serie son tratadas como si fueran símbolos de la cultura pós-moderna; son idealizados en las películas, en los libros y series televisivas además de ser objetos de culto, por muchos, por su inteligencia, capacidad de encantamiento y habilidad para engañar, características que pactúan con los lazos sociales perversos observados en la contemporaneidad.

Diferenças em populações de células exterminadoras naturais (Natural Killers-NK) sanguíneas periféricas entre atletas de caiaque e não atletas / Differences in peripheral blood Natural Killer cell populations between elite kayakers and non-athletes

INTRODUÇÃO: O exercício estressante prolongado tem sido associado a uma depressão transitória da função imune, com rotinas de treinamento e competição intensas e prolongadas capazes de levar os atletas a uma deficiência imune. OBJETIVO: O objetivo deste estudo foi observar se o treinamento cr ônico foi capaz de produzir diferenças sustentáveis no sangue periférico (SP) subpopulações de leucócitos (LEU, granulócitos, monócitos, linfócitos totais, linfócitos B e T, e células CD4+ e CD8+T e células natural killers) de atletas de caiaque de elite quando comparados com não atletas. MÉTODOS: A amostra incluiu 13 homens atletas de caiaque de elite, 20 ± 3 anos, 75,0kg ± 7,9 peso e 177,3 ± 7,1 cm estatura. O VO2max foi 58,3 ± 7,8mL.kg.min-1. O grupo de não atletas incluiu sete homens saudáveis, idade 18 ± 1 ano de idade, 81,3 ± 13,8Kg de peso corporal e 171,9 ± 4,5cm de estatura. As amostras de sangue dos atletas foram coletadas no início da temporada de treinamento, após um período fora do treinamento de seis semanas. Populações de células sanguíneas periféricas foram identificadas por análise de citometria de fluxo. Para identificar as diferenças entre os grupos de atletas e não atletas, o teste U de Mann-Whitney foi utilizado. RESULTADOS: N ão foram identificadas diferenças entre os atletas de caiaque treinados e não atletas em repouso, exceto para células natural killers (CD3-CD56+) e os valores da subpopulação CD3-CD56+CD8+ os quais foram mais baixos nos atletas. CONCLUSÃO: Nosso estudo encontrou que, após um período prolongado sem treinamento (seis semanas), somente a população de NK CD3-CD56+ e, em especial, a subpopulação de altamente citotóxica CD3-CD56+CD8+ apresentou níveis mais baixos nos atletas de elite quando comparados com os homens destreinados.

INTRODUCTION: Prolonged strenuous exercise has been associated with a transient depression of immune function, with prolonged intense training schedules and competition able to lead to immune impairment in athletes. OBJETIVE: The objective of this study was to see if chronic training was able to produce sustained differences in the peripheral blood (PB) leukocyte subpopulations (WBC, granulocytes, monocytes, total lymphocytes, B and T lymphocytes, CD4+ and CD8+ T cells and Natural Killer cells) of elite kayakers when compared to non-athletes. METHODS: The sample comprised 13 elite male kayakers, 20 ± 3 years old, 75.0 kg ±7.9 weight and 177.3±7.1 cm stature. The VO2max was 58.3±7.8 mL.kg.min-1. The Non-athlete group comprised 7 health males, aged 18±1 years old, 81.3±13.8 kg of weight and 171.9±4.5cm stature. The athlete's blood samples were collected at the beginning of the training season, after an off period of six weeks of training. Peripheral blood cell populations were identified by flow cytometry analysis. To verify the differences between the athlete and non-athlete groups the Mann-Whitney U Test was used. RESULTS: No differences between the trained kayakers and the non-athletes were found at rest except for Natural Killer cells (CD3-CD56+) and the CD3-CD56+CD8+ subset values that were lower in the athletes. CONCLUSION: Our study found that after a prolonged time without training (six weeks) only the NK CD3-CD56+ population and particularly the highly cytotoxic CD3-CD56+CD8+ subpopulation had lower levels in the elite athletes when compared to the untrained men.

Cytokine-induced Killer Cell Activation by Dentritic Cells Loaded with the Tumor Antigen from Human Lung Cancer Cell Line A549 / 中国慢性病预防与控制

Objective To explore the specific anti-tumor effect of cytokine-induced killer cell (CIK) activation by dentritic cells (DC) loaded with the tumor antigen from human lung cancer cell line A549. Methods CIKs and DCs derived from the health donor's peripheral blood monocyte (PBMC) were isolated and induced by the cytokine in vitro. DCs were generated by culture of adherent cells from PBMC for 7days in the presence of IL-4 and GM-CSF. CIKs were generated by culture of non-adherent cells for 7 or 14 days in the presence of INF-?, IL-2, IL-1?, and mouse anti-human CD3 monoclonal antibody. The phenotype of DC and CIK was analyzed by FCM. The activity of DC was evaluated by MLR; and the cytotoxicity of CIK was assayed by MTT. The CIK expression profile of cytokine and signal transduction genes were detected by the DNA micro-array under different circumstances. Results Phenotypic analysis indicated that CD3+CD56+NKT cells were predominant in the CIK cells generated in the current study, and that DC cells expressed high levels of CD40, CD80, CD86 and HLA-DR. As demonstrated by MTT assay, the CIK cells proliferated rapidly, and the DC cells were able to induce an MLR. Both antigen-pulsed and unpulsed DC stimulated the proliferation of CIK cells, and no significant difference was found between the two kinds of DC cells. Unpulsed DC cells did not enhance cytotoxicity mediated by CIK cells even though they were able to stimulate CIK proliferation. Antigen-pulsed DC, however, stimulated CIK cells to specifically kill target cells. The specific antitumor effect was also observed in nude mice bearing tumors. Conclusion One of the major effects of antigen-pulsed DC is to activate CIK cells and to make them specific in killing tumor cells.

Research on PHA-CIK cells' immunophenotype and cytotoxicity / 中国免疫学杂志

Objective:To research PHA-CIK cells' phenotype and cytotoxicity. Methods: Using phytohemagglutinin(PHA)to stimulate peripheral blood mononuclear cells(PBMNCs) for 24 h,then cultured like incubating cytokine induced killers by traditional method. On 15th day examined its immunophenotype by flow cytometry and using MTT method to evaluate cytotoxicity. Results: The ratio of CD3+,CD8+, CD3+ CD8+ and CD3+ CD56+ cells were high.PHA-CK cells cytotoxicity was strong in some degree.Conclusion:PHA-CIK cells had strong cytotoxicity.The result provides an experimental basis for biotherapy.