RELATIONSHIP BETWEEN THE LEU-ENKEPHALIN-LIKE IMMUNOREACTIVE NEURONS OF THE HYPOTHALAMUS AND ITS ADJACENT AREA AND THE POSTERIOR PITUITARY IN THE RAT / 解剖学报

Leu-enkphalin-like(L-ENK-L)neurons of the hypothalamus and its adjacentarea of the rat were stained immunohistochemically.It has been found that besidesthe area previously reported in the literature L-ENK-L neurons are also present inSFO and the ependyma of the third ventricle.By combining WGA-HRP retrogradetracing and immunohistochemical method,L-ENK-L neurons in supraoptic nucleus,paraventricular nucleus,periventricular area and anterior fornical nucleus were foundto project to the posterior pituitary,in which some neurons squeeze in between theependymal cells.The subependymal neurons may serve as a link between the ven-tricle and the posterior pituitary.

THE OBSERVATIONS ON 5-HT-, SP-, AND LENK-LIKE IMMUNOREACTIVE ULTRASTRUCTURES OF THE VENTROLATERAL SUBDIVISION OF MIDBRAIN PERIAQUEDUCTAL GRAY IN THE RAT / 解剖学报

5-HT-, SP- and L-ENK-like (5-HT-LI, SP-LI and L-ENK-LI) immunoreactive ultrastructures of the ventrolateral subdivision (VLS) of midbrain periaqueductal gray (PAG) in the rat were observed by immunoelectron microscopical technique in the present study. 5-HT-LI neuronal cell bodies were frequently seen in the VLS of the PAG. 5-HT-LI dendrites were found to form axo-dendritic synapses with non-immunoreactive axon terminals, and the major form of this kind of synapse was asymmetric. A few 5-HT-LI axon terminals formed axo-axonic synapses with non-5-HT-LI axon boutons. 5-HT-LI and non-5-HT-LI axon boutons formed axo-dendritic and axo-somatic synapses with 5-HT-LI dendrites, non-5-HT dendrites and 5-HT-LI neuronal cell bodies, respectively. SP-LI fusiform neuronal cell bodies were only a few and formed axo-somatic synapses with non-SP-LI synaptic boutons which contained pleomorphic vesicles. SP-LI dendrites formed axo-dendritic synapses with non-SP-LI axon terminals, this kind of synapse was the main form of synapses formed by SP-LI structures. SP-LI axon boutons formed axo-somatic and axo-dendritic synapses with non-SP-LI neuronal cell bodies and SP-LI dendrites. L-ENK-LI neuronal cell bodies were also limited. The most common form of synapses of L-ENK-LI structures was L-ENK-LI dendrites formed axo-dendritic synapses with non-L-ENK axon terminals. Non-L-ENK-LI axon terminals constituted axo-somatic synapses with L-ENK-LI neuronal cell bodies. A few L-ENK-LI axon terminals formedaxo-dendritic synapses with L-ENK-LI dendrites. The majority of the synapses mentioned above contained spherical clear vesicles, but some were mixed with a few flat, oval and granular vesicles. The immunoreacrive products were located on the surface of vesicles or on the surface of membranous organelles in the cytoplasm.

DISTRIBUTION AND DEVELOPMENT OF SUBSTANCE P(SP)-, L-ENKEPHALIN (L-ENK)-AND SEROTONIN(5-HT)-LIKE IMMUNO-REACTIVITY IN GUDDEN'S DORSAL TEG-MENTAL NUCLEUS IN THE HUMAN FOETUS AND NEONATE——AN IMMUNOHISTO-CHEMICAL STUDY / 解剖学报

Using immunohistochemical technique, the distribution and development of substance P (SP)-, L-enkephalin (L-Enk)- and serotonin (5-HT)-like immunoreactivity in Gudden's dorsal tegmental nucleus (GDTN) of human foetus (fetal age 11.5 to 35 weeks)and neonate (two days) were demonstrated. Gudden's dorsal tegmental nucleus in the human might be divided into pars centralis and pars peripheralis; and the time of initial appearance for SP-, L-Enk- and 5-HT-like immunoreactivity in GDTN was different. SP-like immunoreactive fibers and terminals appeared first and were distributed mainly in the pars centralis and their density was very high. L-Enk-like imunoreactive cells, fibers and terminals were distributed in the pars peripheralis. 5-HT-like immunoreactive cells, fibers and terminals were distributed in the medial part of the GDTN; As the foetus developed further, SP-, L-Enk- and 5-HT-like immunoreactivity in the GDTN showed their own special ontogenetic changes respectively

AN IMMUNOCYTOCHEMICAL AND RADIOIMMUNOASSAY STUDY ON THE DISTRIBUTION OF VIP,SP,L-ENK AND SOM IN THE SMALL INTESTINE OF THE RAT / 解剖学报

SOM in sequence.The results of RIA wereconsistent with the density of the peptide-containing structures by ICC.