Inhibition of photoreceptor apoptosis in mice with retinitis pigmentosa through NF-κB/NLRP3 pathway suppression with Lycium barbarum polysaccharide / 数字中医药（英文）

Objective@# To explore whether Lycium barbarum polysaccharide (LBP) can reduce the apoptosis of retinal photoreceptor cells in retinitis pigmentosa (RP) mice by inhibiting nuclear factor-kappa B (NF-κB)/NOD-like receptor thermal protein domain-associated protein 3 (NLRP3) signaling pathway. @*Methods@# (i) In vitro experiments, mouse retinal ganglion cells (661W cells) were divided into normal, model, LBP low-dose (LBP-L, 40 mg/L), LBP middle-dose (LBP-M, 80 mg/L), LBP high-dose (LBP-H, 160 mg/L), and positive drug control (NLRP3 inhibitor, 160 mg/L) groups. And the 661W cells were exposed to varying concentrations of H2O2 ranging from 50 to 400 μmol/L to determine the optimal concentration for inducing apoptosis (200 μmol/L). Then the cell viability was assessed using Cell Counting Kit-8 (CCK-8), while the apoptosis rate was detected by flow cytometry; the expression of NLRP3 was detected by immunofluorescence; and the expression of apoptosis markers was detected by enzyme-linked immunosorbent assay (ELISA) and Western blot (WB). (ii) In vivo assays were carried out with the use of C57/BL6 and Rd10 mice. The animal experimental groups were divided into normal, model, LBP-L, LBP-M, LBP-H, and NLRP3 inhibitor groups, in which the normal group was C57/BL6 mice and the other groups were Rd10 mice. Ten mice were included in each group, and the corresponding drugs were administered intragastrically for a duration of four weeks. NF-κB/NLRP3 pathway and the expression of apoptosis markers were observed by electroretinogram, histopathological examination, and WB to assess the effects of LBP on retinal photoreceptor cell apoptosis.@*Results@#(i) In vitro experiments, compared with the normal group, the apoptosis rate of 661W cells in model group was significantly increased (P < 0.01), and the expression levels of key proteins of NF-κB/NLRP pathway, such as NLRP3, NF-κB, p-NF-κB, and pro-apoptotic protein caspase-3, were up-regulated (P < 0.01). The rate of Bax/Bcl-2 was increased (P < 0.01), and the concentrations of interleukin (IL)-1β and tumor necrosis factor (TNF)-α were significantly increased (P < 0.01). Compared with the model group, high dose of LBP decreased the apoptosis rate of 661W cells (P < 0.01), and down-regulated the expression levelsof the key proteins of NF-κB/NLRP3 pathway, including NF-κB, NLRP3, p-NF-κB, and caspase-3 (P < 0.01). The rate of Bax/Bcl-2 was decreased (P < 0.01), and the concentrations of IL-1β and TNF-α were decreased (P < 0.01). (ii) In vivo experiments, high dose of LBP significantly increased morphological changes in the outer nuclear layer (ONL) thickness of Rd10 mice, as well as functional changes in the amplitudes of the a-wave and b-wave (P < 0.01), which also down-regulated the expression levels of NF-κB (P < 0.05), NLRP3, p-NF-κB, and caspase-3 (P < 0.01), reduced the Bax/Bcl-2 rate (P < 0.01), and decreased the concentrations of IL-1β (P < 0.01) and TNF-α (P < 0.05). @*Conclusion@#LBP could improve both retinal morphology and function, providing protection to photoreceptors from apoptosis through the inhibition of the NF-κB/NLRP3 pathway.

Live biotherapeutic products: the forefront of innovative drug development driven by biotechnology / 生物工程学报

As human microbiome research advances, a large body of evidence shows that microorganisms are closely related to human health. Probiotics were discovered and used as foods or dietary supplements with health benefits in the last century. Microorganisms have shown broader application prospects in human health since the turn of the century, owing to the rapid development of technologies such as microbiome analysis, DNA synthesis and sequencing, and gene editing. In recent years, the concept of "next-generation probiotics" has been proposed as new drugs, and microorganisms are considered as "live biotherapeutic products (LBP)". In a nutshell, LBP is a living bacterial drug that can be used to prevent or treat certain human diseases and indications. Because of its distinct advantages, LBP has risen to the forefront of drug development research and has very broad development prospects. This review introduces the varieties and research advances on LBP from a biotechnology standpoint, followed by summarizing the challenges and opportunities for LBP clinical implementations, with the aim to facilitate LBP development.

Comparison Of Low Back Extensors, Abdominal Core And Abdominal Muscle Endurance In Normal And Overweight Young Females

Background: High Body Mass Index(BMI) has been linked with increased risk of low back pain(LBP) in females. High BMI has been linked with an increased risk of low back pain. Increased body fat could increase the mechanical load on the spine by causing a higher compressive force or increased shear on the lumbar spine structures during various activities. In LBP, the muscular endurance of the back muscles mainly, the abdominal core, back extensors and abdominals is reduced. Hence, there is a need to assess muscular endurance of the trunk muscles in overweight females so as to determine a cause for LBP. The objective of this study is to compare back extensors, the abdominal core and abdominal muscle endurance in overweight and normal females in the age group of 20-30years. Method: A group of 120 healthy females were included in this study. Out of these, 60 females were with normal BMI and 60 were overweight. Back extensor endurance was evaluated by Beiring-Sorenson’s test. The core muscle endurance was assessed by Sphygmomanometer method in prone lying. In both the tests, holding time was measured by stopwatch. The abdominal muscle endurance was assessed by Partial curl-up test. Curl-ups were done according to the metronome beats. The number of repetitions performed was measured. A comparison was done between normal and overweight females. Results: In normal females the mean age was 21.18 with mean BMI of 21.96 whereas in overweight females the mean age was 21.27 with mean BMI of 27.44. The mean back extensor, core and abdominal endurance in normal females were 59.053,10.81 and 18.97 respectively was high as compared to overweight which was 30.33,6.13 and 11.27 respectively. Data analysis was done using unpaired t-test. The muscular endurance was significantly reduced (p<0.00) in overweight females as compared to the normal females. Conclusion: This study concluded that back extensor, core and abdominal endurance are significantly reduced in overweight females as compared to normal.

Does Motor Control Exercise Decreases Lumbar Pain, Improves Muscle Activity And Regional Function In Individuals With Acute And Sub Acute Non-Specific Low Back Pain

Background: Non-specific low back pain (LBP) is defined as LBP that poses signs and symptoms which cannot be related to a recognizable cause, and Motor Control Exercise (MCE) usually is the choice of treatment for conditioning lumbar muscles for chronic LBP group. Limited information is available regarding their clinical application for participants with acute and sub-acute LBP. Hence, the main aim of this study is to find out this clinical utility. Methods: A quasi-experimental study with 30 participants of less than six weeks and twelve weeks duration of LBP were included in the study and are divided into an experimental and control group. Pain intensity using numeric pain rating scale (NPRS), lumbar range using modified Schober’s test, muscle function using surface electromyography (EMG) and functional disability using Roland Morris Disability Questionnaire (RMDQ) were recorded pre and post-treatment. The experimental group received lumbar MCE with general exercises and the control group received only general low back exercises aiming to improve lumbar range and muscle efficiency for six-session spread over three weeks duration along with therapy for pain reduction. Results: Subjects in both experimental & control groups had significant improvement in pain (p<.001) and RMD Questionnaire (P<.001), Lumbar range of motion had improved significantly only in the experimental group (Flexion p<.001, Extension p<.001) compared to control group. Though lumbar muscle activation had improved in both the groups, subjects in the experimental group showed significant and uniform improvement in lumbar muscle activation following MCE than the control group. Conclusion: Motor Control Exercise provides better clinical improvement in pain, lumbar muscle activation and regional functional ability without exacerbating symptoms in subjects with LBP during the acute and sub-acute phase.

A Cross-Sectional Study Determining Pain in Lower Back and Calf Muscles among Females Due to High Heel Shoe Wear

To determine pain in lower back and calf muscles among females due to high heels shoe wear. Methodology: A sample size of 87 females working women and students were recruited on the basis of the purposive sampling technique. Participants were requested to wear 5 cm stiletto high heels and they were asked to walk on treadmill at the pace of 4km/hr for 20 minutes. Modified McGill Questionnaire for pain measurement was used as a tool before and after test data collection. Results: In this study observation of muscular pain was significant in lower limb and lower back muscles due to high heels i.e. 29.6% and 24.7% respectively. Conclusion: Back and lower limbs musculature pain contribute only 8.6% whereas, only back pain had been observed in 9.9%. Paired sample t-test was used to determine pain before and after test was significant with p-value 0.000.

Effect of Lycium barbarum polysaccharides on inflammatory cytokines in type 2 diabetes mellitus model mice without myeloid differentiation factor 88 gene / 上海交通大学学报（医学版）

Objective: To investigate the effect of Lycium barbarum polysaccharides on inflammatory cytokines in type 2 diabetes mellitus (T2DM) mice without myeloid differentiation factor 88 gene (MyD88-/-). Methods: Levels of interleukin 1β (IL-1β), IL-6, IL-8, transforming growth factor β1 (TGF-β1), and IL-10 in serum were assessed by ELISA in the MyD88-/- T2DM mice which had been administered with different doses of LBP (20, 40, and 80 mg/kg). Mouse macrophages Raw264.7 were stimulated by lipopolysaccharide (LPS) after treatment with different concentrations of LBP (25, 50, and 100 μg/mL). Then Western blotting was used to detect nuclear translocation level of nuclear factor κB (NF-κB) and protein expressions of inhibitor of NF-κB (IκB) and p-IκB. Results: Serum levels of IL-1β and TGF-β1 in MyD88-/- T2DM mice were down-regulated by LBP (P<0.05). Cell experiment proved that nuclear migration of NF-κB was dose-dependently inhibited by LBP, and the level of p-IκB was reduced by high dose of LBP. Conclusion: LBP can reduce some proinflammatory cytokines in the MyD88-/- T2DM mice, which may be related with its inhibitive effect on the phosphorylation of IκB and nuclear migration of NF-κB in the macrophages.

Trans-Sacral Epiduroscopic Laser Decompression (SELD) for Low back pain secondary to herniated lumbar disc in the Philippines: A case report and review of literature

@#LBP has a lifetime prevalence ranging from 54% to 80%, an annual prevalence of 15-45%, and a point prevalence of 30%. Lumbar radicular pain often results from a Lumbar disc herniation. With recent advances in technique and access in instrumentation, minimally invasive spine surgery has ushered in a renaissance of spine care. SELD has promising positive effects in controlling LBP following HLD. This is a case of a 26 year old with radicular LBP of 2 years duration secondary to HLD, underwent conservative management of LBP but offered no relief and improvement, hence he became the case for the pioneering procedure of SELD in the Philippines. Immediate and significant improvement in the patient was noted. SELD was proven to be an effective therapeutic modality for patients with LBP secondary to HLD.

Protective effects of LBP on cerebral ischemia reperfusion injury in mice and mechanism of inhibiting NF-κB, TNF-α, IL-6 and IL-1β / 中国中药杂志

To observe the protective effects of Lycium barbarum polysaccharides (LBP) on cerebral ischemia reperfusion injury in mice and explore its mechanism. Common carotid artery thread was used to cause middle cerebral artery ischemia, and the thread was taken out after 2 h ischemia to achieve cerebral ischemia reperfusion injury in mice. Therefore, the transient middle cerebral artery occlusion (tMCAO) models were established to observe the effects of LBP (25,50, 100 mg•kg⁻¹) on neurological outcome, infarct size and water contents. HE staining was used to observe its effects on neurocytes of cerebral tissues in mice. Western blotting was used to evaluate the protein expression levels of NF-κB p65. ELISA was used to evaluate the levels of TNF-α, IL-6 and IL-1β in the serum. According to the results, LBP markedly improved neurologic deficits, and decreased infarct size and water contents at 24 h after reperfusion in mice. Pathological section of brain tissues also proved its protective effects on neurocytes. Western blot analysis indicated that LBP markedly down-regulated the protein level of NF-κB p65. ELISA indicated that LBP decreased the levels of TNF-α, IL-6 and IL-1β in the serum 24 h after reperfusion.In conclusion, LBP has protective effects on cerebral ischemia reperfusion injury in mice, and this effect may be associated with inhibiting NF-κB and inflammatory reactions.

Relación entre el dolor lumbar y los movimientos realizados en postura sedente prolongada: Revisión de la literatura / The relationship between low back pain and movements in prolonged sitting posture: Literature review

La evidencia científica muestra que existe asociación entre dolor lumbar y postura sedente prolongada y que una de las poblaciones más propensa a esta enfermedad es la de conductores, con una alta prevalencia. De igual manera, los movimientos posturales en la silla son la respuesta natural del cuerpo debida a la incomodidad percibida por el dolor lumbar, pero esta difiere en función de la historia previa. Con base en esto se realizó una revisión de los estudios relacionados con el dolor lumbar y el movimiento en postura sedente prolongada, incluyendo la población de conductores, con el fin de comprender esta relación. Se recuperaron 6226 artículos, de los cuales 16 fueron publicados entre 1970 y 2010; estos proporcionaron la información necesaria para contestar la pregunta de revisión. Se pudo constatar que ninguno de los estudios fueron realizados en actividades de conducir y la mayoría involucró sujetos voluntarios sin considerar si la población era trabajadora. El movimiento con dolor lumbar en postura sedente prolongada solo se pudo explicar desde el rango de movimiento lumbar, debido a que solo se encontró un artículo con estas características. También se encontró que los sintomáticos se mueven más, pero que el dolor lumbar no disminuye en función del tiempo, es decir que conocemos muy poco acerca de la paradoja movimiento y dolor en postura sedente prolongada.

Scientific evidence shows an association between low back pain and prolonged sitting posture, also one of the most sensitive populations to this disorder are the drivers with a high prevalence. Postural movements in the chair are body's natural response to perceived discomfort caused by back pain but this is different depending on each individual's background of back pain. Based on this, we perform a review of studies related to low back pain and movement in prolonged sitting posture, including the driver population, in order to understand this relationship. We retrieved 6226 articles, of which 16 matched the established criteria and provided the information necessary to answer the review question. We found that none of the studies considered were conducted in driving activities and most of them were done with volunteer subjects regardless if they were workers or not. The movement with low back pain in prolonged sitting posture could only be explained from the range of lumbar motion because only one article matched these characteristics, finding that symptomatic people move more but low back pain does not decrease as a function of time, proving that we know very little about the paradoxical between movement and pain in prolonged sitting posture.

Clinical significance of HPV subtype infection and atypical glandular cells on LBP / 国际检验医学杂志

Objective To research the clinical outcome of atypical glandular cell (AGC)according to various subtypes of HPV infection and histological pathology results.Methods The data of the liquid-bases cytology (LBP),HPV infection and histology in 102 cases of AGC at the gynecology outpatient department of our hospital from January 1 ,2009 to February 28,2014 were collected and performed the analysis on their clinical outcomes.Results Among 67 218 cases of LBP detection,102 cases were AGC with the total incidence rate of 0.15%.In the cases of AGC-NOS,67 cases were normal or benign lesions,11 cases were precancerous lesion and malignant lesions;in the cases of AGC treading to tumor,the benign,precancerous and malignant lesions were in 7,14 and 3 ca-ses respectively.At the same time in the cases of AGC-NOS,HPV infection was in 64 cases,in which 57 cases were high risk infec-tion(type 16,52,45)and 7 cases were low risk infection(type 6,11 ).The single infection,double infection and multiple infection were in 54,6 cases respectively;in the cases of AGC trends to neoplasm,HPV infection was in 19 cases,in which 18 cases were high risk infection(type 52,16,18)and 1 case was low risk infection(type 6),single infection and double infection were in 15 cases and 4 cases respectively.Conclusion AGC may play an important role for the forecast of cervical malignant lesions.The results of differ-ent HPV subtypes infection in AGC related tumors also play a certain role in the prediction of cervical neoplasia.Their combined a-nalysis is the important signal for judging the occurrence of gynecological cervical precancerous lesion and malignant tumor,i.e., AGC combining with the corresponding HPV subtype infection not only can make a judgement for the cervical lesions,but also pro-vides the basis for predicting the high risk existence of gynecological malignant tumor and provides constructive suggestions for Chi-na regional cervical carcinoma vaccine manufacturing and promotion.

Protective effects of Lycium barbarum polysaccharide on rat hippocampal neurons injured by oxygen-glucose deprivation and reperfusion / 中国药学杂志

OBJECTIVE: To investigate the protective effects of Lycium barbarum polysaccharide(LBP) against injury from oxygen-glucose deprivation/reperfusion (OGD/RP) in primary cultured rat hippocampal neurons. METHODS: Cultured hippocampal neurons were exposed to oxygen-glucose deprivation (OGD) for 2 h followed by a 24 h reperfusion. The MTT assay and the lactate dehydrogenase (LDH)release were used to evaluate the protective effects of LBP. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA)were determined by spectrophotometry using commercial kits. Mitochondrial membrane potential (MMP) and the intracellular free calcium concentration ([Ca2+]i) in hippocampal neurons were measured using confocal laser scanning microscope (CLSM). RESULTS: Treatment with LBP (10-40 mg · L-1) significantly attenuated neuronal damage and inhibited LDH release in a dose-dependent manner. Furthermore, LBP enhanced activities of SOD and GSH-Px but it decreased their MDA content, inhibited [Ca2+]i elevation and decrease of MMP in ischemia-reperfusion treated hippocampal neurons. CONCLUSION: These findings suggested that LBP may be a potential neuroprotective agent for cerebral ischemia-reperfusion injury.

Recognition of lipopolysaccharide pattern by TLR4 complexes

Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria. Minute amounts of LPS released from infecting pathogens can initiate potent innate immune responses that prime the immune system against further infection. However, when the LPS response is not properly controlled it can lead to fatal septic shock syndrome. The common structural pattern of LPS in diverse bacterial species is recognized by a cascade of LPS receptors and accessory proteins, LPS binding protein (LBP), CD14 and the Toll-like receptor4 (TLR4)-MD-2 complex. The structures of these proteins account for how our immune system differentiates LPS molecules from structurally similar host molecules. They also provide insights useful for discovery of anti-sepsis drugs. In this review, we summarize these structures and describe the structural basis of LPS recognition by LPS receptors and accessory proteins.

Influência da Escola de Postura na qualidade de vida, capacidade funcional, intensidade de dor e flexibilidade de trabalhadores administrativos / Influence of the Back School on quality of life, functional capacity, intensity of pain and flexibility of administrative workers

O objetivo do estudo foi analisar os efeitos de um programa "Escola de Postura" em relação à qualidade de vida, capacidade funcional, intensidade de dor e flexibilidade em trabalhadores com dor lombar inespecífica. Participaram 33 trabalhadores do setor administrativo. O programa foi realizado em sete encontros com quatro grupos (com sete ou oito participantes), uma vez por semana, com duração de uma hora cada. Antes e após a intervenção, os voluntários responderam ao questionário de qualidade de vida Short-Form Health Survey (SF-36) e ao questionário de incapacidade funcional Roland-Morris, realizaram o teste sentar e alcançar com o banco de Wells e assinalaram a intensidade de dor na escala visual analógica. O teste estatístico de Shapiro-Wilk foi usado para analisar a normalidade de distribuição dos dados. Os dados foram analisados pelo teste Wilcoxon e pelo teste t de Student, com nível de significância de 5% (p<0,05). Foi observada melhora da qualidade de vida em sete domínios do SF-36 (p<0,005), da incapacidade funcional (p<0,005), da intensidade de dor (p<0,005) e da flexibilidade (p<0,005). O índice de adesão foi de 58,93%. O programa "Escola de Postura" proposto melhorou significativamente a qualidade de vida, capacidade funcional, flexibilidade e intensidade de dor de adultos trabalhadores de setores administrativos.

The purpose of this study was to analyze the effects of the "Back School" in the quality of life, functional capacity, pain intensity and flexibility for workers with nonspecific low back pain. Thirty-three administrative sector workers participated. The program was conducted in seven meetings with four groups (seven to eight subjects) once a week, lasting one hour each. Before and after the intervention, the subjects answered a questionnaire on quality of life Short-Form Health Survey (SF-36), the incapacity functional questionnaire, Roland Morris, performed the sit and reach test with the Wells' bank, and noted the intensity pain in visual analog scale. The statistical test of Shapiro-Wilk test was used to analyze the normality of data distribution. The data were analyzed by Wilcoxon and Student's t-test with significance level of 5% (p<0.05). In the improvement of quality of life in seven domains of the SF-36 (p<0.005), functional incapacity (p<0.005), pain intensity (p<0.005) and flexibility (p<0.005). The rate of adherence was 58.93%. The "Back School" program offered significantly improved the quality of life, functional capacity, flexibility and intensity of pain in adult administrative workers.

A risk prediction model of the incidence of occupational low back pain among mining workers.

Background: Low Back Pain (LBP) is the most frequently reported musculoskeletal disorder in workers. This study was aimed to develop risk prediction model of low back pain that can be used to prevent the recurring low back pain attack. Methods: The study was case-control design based on the industrial community by using ergonomical approach. Total samples were 91 workers for cases and 91 workers for controls. Workers suffering for low back pain in the last 6 months served as cases, and those from the same age group and receiving the same amount of exposure without any symptoms of low back pain served as controls. Risk factors include socio-demographic factors, socio-ocupational factors, physical working environmental factors, non-physical environmental factors, and biomechanics factors. Receiver Operating Characreistics (ROC) was used to describe relationship between true positive value (in vertical axis) and false positive value (in horizontal axis) in order to discover a risk predictive value of LBP. Results: The determinant risk factors for low back pain (LBP) were bending work postures, waist rotation movement, manual lifting, unnatural work postures, those who had worked for more than 18 years, and irregular sport activities. By using ROC with 91.20% senstivity and 87.90% spesifi city, the calculated prediction value was 0.35. This is the cut-off point to discriminate workers with and without LBP. The risk predictors value of work-induced LBP calculated by linear equation of logistic regression varied between 0-11.25. Conclusion: The prediction model of work-induced LBP can be used for early detection of LBP to reduce the risk and prevent the recurrence of LBP.

Prevalence and Risk Factors Associated with Low Back Pain Among Health Care Providers in a District Hospital

Study design: A cross sectional study among health care providers working at one hospital. Objective: To investigate the prevalence, the consequences and the risk factors associated with low back pain (LBP) among hospital staff. Materials and Methods: The study sample consisted of 931 health care providers who answered a pre established questionnaire including 30 items in two languages. Results: The cumulative life prevalence of LBP was 72.5% and the yearly prevalence was 56.9%. Chronic LBP prevalence was

A Study on Characteristic Factors Related to Low Back Pain and Mental Health of Pregnant Women

PURPOSE: The study was to explore the level of low back pain and characteristic factors influencing low back pain (LBP) and mental health during pregnancy. METHODS: The subjects were a total of 383 healthy pregnant women in S City and K-Do. Data were analyzed using descriptive statistics, t-test, ANOVA, and Pearson's correlation. RESULTS: 82.5% of the pregnant women answered the existence of LBP and 19.7% of them had high LBP. The preferred method of controlling LBP was 'Just endure' (42.3%). There were significant differences in pregnancy level (p < .05) and discomfort condition related to pregnancy (p < .01) according to low back pain. There were significant differences in pain intensity according to mental health. The correlation between pain level and pregnancy weeks (p < .001) and BMI in previous pregnancy (p < .001) was significant. The correlation between mental health and age was significant (p < .001). CONCLUSION: The majority of the pregnant women experienced LBP during pregnancy. However, they were not offered the best method of controlling the pain. Thus, for preventing LBP during pregnancy, we recommend regular exercises and BMI control.

The Effects of Self Foot Reflexology on Depression, Sleep and Low Back Pain in Community Dwelling Elderly Women / 기본간호학회지

PURPOSE: The purpose of this study was to identify the effects of self foot reflexology on depression, sleep, and low back pain in elderly women. METHOD: The study was one group pre-posttest design. Data collection was done from December 2007 to January 2008 in D city. The participants were 47 women (65 years of age and over). After two weeks of reflexology education, the women did foot reflexology themselves for approximately 40 minutes, five times a week for four weeks. To verify the effects of foot reflexology, the scores for depression, sleep, and LBP were measured at baseline, after 4 weeks of self-treatment. Repeated measure ANOVA with the SPSS WIN 14.0 program were used to analyze the data. RESULTS: There were significant differences in depression, sleep, and LBP between baseline, after 4 weeks of self-treatment. CONCLUSION: These findings suggest that self foot reflexology could be utilized as an effective nursing intervention for improving depression, sleep, and LBP in elderly women.

Change of lipopolysaccharide activity-modulating protein in serum of cirrhosis patients and its clinical significance / 中国康复理论与实践

@#ObjectiveTo explore changes of lipopolysaccharide (LPS), lipopolysaccharide-binding protein (LBP), bactericidal/permeability-increasing protein (BPI), soluble CD14 (sCD14) in blood of cirrhosis patients and their clinical significance.MethodsSerum samples of 45 cirrhosis patients were detected with chromogenic limulus amebocyte lysate assay for LPS and detected with ELISA for LBP, BPI and sCD14. While, serum samples of 15 normal subjects were used as controls.ResultsLevels of LPS, LBP, BPI and sCD14 in blood of cirrhosis patients with liver function being grade A, B and C were significantly higher than that in normal subjects. Also, those indexes fore mentioned were obviously higher in died cirrhosis patients than that in survived cirrhosis patients.Conclusion High levels of LBP, LPS and relative deficiency of BPI in cirrhosis patients accompanied with intestinal endotoxemia (IETM) may significantly increase the sensitivity of body to endotoxin.

Screening and identification of phage antibodies against human lipopolysaccharide binding protein / 第三军医大学学报

Objective To obtain and identify monoclonal antibody against human lipopolysaccharide binding protein (LBP). Methods Three clones of antibody with better activity against human LBP were isolated after human antibody library screening by human LBP and 5 rounds of enrichment. After plasmid extracting, geneⅢ cutting, self-recircling and electric transforming, soluble Fab was expressed in E.coli. XL1-blue by the induction of IPTG and its characteristics were determined. Results The positive antibody was concentrated to 8.16?104 folds and the highest activity of the three clones was 106 . Conclusion Phage antibody against human LBP has been obtained successfully, which lays a solid foundation for researching its function.

Proteins and signaling pathway in endotoxic effect / 中国药理学通报

This article reviews the proteins and transduction pat hw ay in endotoxic action. Lipopolysaccharide-binding protein(LBP) binds and trans ports the LPS to its receptors, which include CD14 and CD11/CD18. Toll-like rec eptors(TLRs) is closely associated with transducting the signals into cytoplasm. Scavenger receptors are related to hepatic clearance on endotoxin .The intracel lular signal transduction is involved in several paths which finally leads to t he release of cytokines.