Standardized management of central medical laboratory based on informatized website / 医疗卫生装备

Objective To eliminate the problems due to manual operation in the central medical laboratory.Methods Management of the central medical laboratory was enhanced by ideas of informatization and standardization,informatized website,standardized transaction management as well as cooperation of laboratory staffs.Results Informatized website gained advantages over the traditional management mode in electronic record,information capacity,conciseness,timely feedback and etc.Conclusion Standardized management of central medical laboratory based on informatized website contributes to enhancing scientific research environment and application efficiency of large devices.

Standardized management of central medical laboratory based on informatized website / 医疗卫生装备

Objective To eliminate the problems due to manual operation in the central medical laboratory.Methods Management of the central medical laboratory was enhanced by ideas of informatization and standardization,informatized website,standardized transaction management as well as cooperation of laboratory staffs.Results Informatized website gained advantages over the traditional management mode in electronic record,information capacity,conciseness,timely feedback and etc.Conclusion Standardized management of central medical laboratory based on informatized website contributes to enhancing scientific research environment and application efficiency of large devices.

Digital forensics: A technological revolution in forensic sciences.

Computer Forensics World is a growing community of professionals involved in the digital forensics industry. It is an open resource, free for all to access and to use. Digital investigations and crime regularly cross international and language borders today. Open database connectivity technology is now providing access to a wide range of database technologies, such as neural networks and pattern recognition databases, which are being used to analyze shoe prints and tool marks. These new computer-aided analysis tools can link and chart case information, allowing the investigator to question the data and pose scenarios as well as suggest and follow possible investigative paths. As a result, connections that often took weeks or months to come to light are revealed in a fraction of the time. Forensics is just beginning to get looked at, but the problems are cost and government is slow to adopt new technology. In this paper we discussed about the kingdom of Digital Forensics and Computer Forensic World. The study consists of Computer Forensic World, Digital Forensics (MEK, AFF, and Keyword Search etc.), and the Future Forensics Labs.

A DNA Microarray LIMS System for Integral Genomic Analysis of Multi-Platform Microarrays

The analysis of DNA microarray data is a rapidly evolving area of bioinformatics, and various types of microarray are emerging as some of the most exciting technologies for use in biological and clinical research. In recent years, microarray technology has been utilized in various applications such as the profiling of mRNAs, assessment of DNA copy number, genotyping, and detection of methylated sequences. However, the analysis of these heterogeneous microarray platform experiments does not need to be performed separately. Rather, these platforms can be co-analyzed in combination, for cross-validation. There are a number of separate laboratory information management systems (LIMS) that individually address some of the needs for each platform. However, to our knowledge there are no unified LIMS systems capable of organizing all of the information regarding multi-platform microarray experiments, while additionally integrating this information with tools to perform the analysis. In order to address these requirements, we developed a web-based LIMS system that provides an integrated framework for storing and analyzing microarray information generated by the various platforms. This system enables an easy integration of modules that transform, analyze and/or visualize multi-platform microarray data.

TGF-beta1-induced PINCH-1-ILK-alpha-parvin complex formation regulates mesangial cell proliferation and hypertrophy

TGF-beta1-induced glomerular mesangial cell (GMC) injury is a prominent characteristic of renal pathology in several kidney diseases, and a ternary protein complex consisting of PINCH-1, integrin-linked kinase (ILK) and alpha-parvin plays a pivotal role in the regulation of cell behavior such as cell proliferation and hypertrophy. We report here that PINCH-1-ILK-alpha-parvin (PIP) complex regulates the TGF-beta1-induced cell proliferation and hypertrophy in cultured rat GMCs. When GMCs were treated with TGF-beta1 for 1, 2 and 3 days, the PIP complex formation was up-regulated after 1 day, but it was down-regulated on day 2. Cell numbers were significantly elevated on day 2, but dramatically decreased on day 3. In contrast, a significant increase in cellular protein contents was observed 3 days after TGF-beta1-treatment. TGF-beta1 induced early increase of caspase-3 activity. In GMCs incubated with TGF-beta1 for 2 days, cytosolic expression of p27(Kip1) was dramatically reduced, but its nuclear expression was remarkably elevated. A significantly decreased expression of phospho-Akt (Ser 473) was observed in the cells treated with TGF-beta1 for 1 day. TGF-beta1 induced early increase of phospho-p27(Kip1) (Thr 157) expression with subsequent decrease, and similar responses to TGF-beta1 were observed in the p38 phosphorylation (Thr 180/Thr 182). Taken together, TGF-beta1 differently regulates the PIP complex formation of GMCs in an incubation period-dependant fashion. The TGF-beta1-induced up- and down-regulation of the PIP complex formation likely contributes to the pleiotropic effects of TGF-beta1 on mesangial cell proliferation and hypertrophy through cellular localization of p27(Kip1) and alteration of Akt and p38 phosphorylation. TGF-beta1-induced alteration of the PIP complex formation may be importantly implicated in the development and progression of glomerular failure shown in several kidney diseases.

Development of Flexible Interface Software for Autoanalyzer using Unidirectional and ASTM Bidirectional Protocol / 대한임상병리학회지

BACKGROUND: The Laboratory Information Management System (LIMS) requires instrument interfacing for good efficiency. However, most instrument interfacing cannot be used easily because instruments have different interface protocols and database systems are different in each hospital LIMS. Therefore, it is necessary to establish flexible interface software that would be useful for various instrument interfacing and can be handled by laboratory workers. METHODS: We categorized the raw data acquisition format of instruments into 7 classification according to the field delimiter, the field position and the test ID, and created software so that anyone could make the interface protocols for any instrument that supports the unidirectional or ASTM interface protocol, according to classification. The software also provides various functions, such as host communications and printing. RESULTS: With this software, we have interfaced 23 instruments without program languages (C, C++, Basic, Pascal, etc.) coding. It took about 1-4 hours for each instrument interface. The software supports a maximum of 8 simultaneous instrument connections with one personal computer. Also, it is possible to retrieve acquisition data from instruments with Microsoft Excel without LIMS. CONCLUSTIONS: The advantages of this software are as follows; 1. Markedly shortens the input time for data generated from automated instruments and reduces errors of manual data entry, 2. Effective increase in host computer performance, 3. Significantly saves time and cost for instrument interfacing. Therefore, this software was considered to be very useful for laboratory instrument interfacing.