Construction and management practice of morphologic experimental center in medical university / 中华医学教育探索杂志

Based on the construction and management practice of the morphologic experimental center in Xi'an Medical University, the achievements in laboratory daily operation and institutional man-agement were summarized in the area of lab rules and regulations, instrument and equipment, experiment teaching, lab environment and safety, lab staff administration and so on. The management work has been refined using the practice model of resource sharing, system administration, individual responsibility, and unified staff supervision. The lab rules and responsibilities were also effectively implemented on specific person. Taking the opportunity in teaching evaluation at the experimental center, the lab connotation con-struction was further strengthened. The evaluation system was thoroughly examined in order to look for gaps and promote the lab construction. Further work could be carried out on the laboratory software and hard-ware, such as instrument and equipment update, experimental teaching system reformation in morphology, promotion on multidisciplinary integration and unified management of lab staff.

Desafios para a rede nacional de laboratórios de vigilância sanitária: o caso dos medicamentos manipulados / Challenges to the national network of sanitary surveillance laboratories: the case of manipulated drugs

A partir da pesquisa "Situação dos medicamentos manipulados (MMs) nos laboratórios de vigilância sanitária (LVS)", produto do convênio Ensp/Anvisa, apresentam-se e problematizam-se elementos que possam contribuir para a reflexão sobre a estruturação da Rede Nacional de LVS no enfrentamento de demandas analíticas, usando os MMs como organizadores da discussão. Identificaram-se os laboratórios públicos (LP) que avaliaram MMs no período de 2000 a 2005, perscrutaram-se seus analistas e as amostras e escolheram-se três laboratórios para visita. Analisou-se o processamento da amostra desde sua entrada até a emissão do laudo. O estudo das amostras de MMs possibilitou identificar: os requerentes e a documentação da amostra; os LVS executores e como recebem, processam e respondem à demanda das solicitações de análises. Os requerentes são o Judiciário (20 por cento) e a Visa (74 por cento). Demandas infundadas somam 25 por cento. Sete LVS analisaram MMs. 45 por cento das amostras passaram antes por 15 LP; RH insuficiente para a complexidade analítica das denúncias; pouca cultura institucional em lidar com amostras de MMs e da Visa; 31 por cento das amostras sem conclusão. 40 por cento dos laudos satisfatórios e 23 por cento dos insatisfatórios não realizaram ensaios adequados à resposta dos motivos da demanda. Os LVS precisam avaliar seu processo de trabalho.

From the research on the "Status of Pharmacy Formulated Medicines (PFM) in health surveillance laboratories (HSL)" as result of an agreement between Ensp and Anvisa, we present and question issues that may contribute for reflections about the National Network of HSL organization in confronting analytical demands, having PFMs as discussion organizers. The Public Laboratories (PL) that evaluated PFMs from 2000 to 2005 were identified; its analysts and samples were scanned, and three laboratories were selected to be visited. Sample processing was analyzed from its entry till the final report's issuance. Samples study allowed identifying: applicants and sample's documents; HSL executors and how they receive, process and reply the analysis requests demands. Applicants are judiciary (20 percent) and the health surveillance (HS) (74 percent). Baseless claims represent 25 percent. Seven HSL analyzed PFMs. 45 percent of the samples previously passed by 15 PL; HR were insufficient to meet the claims' analytical complexity; little institutional culture in dealing with samples of PFM and HS; 31 percent of samples have no conclusion; 40 percent of the satisfactory reports and 23 percent of the unsatisfactory have not conducted adequate tests to answer the demand's reasons. In conclusion, HSLs must evaluate their working process.

Clinical and laboratory evaluation of schistosomiasis mansoni patients in Brazilian endemic areas

A total of 60 percent of the territory of Alagoas (AL) is considered endemic for the occurrence of schistosomiasis and the classification of clinical forms of the disease are not known. This paper aimed to evaluate an endemic schistosomiasis population in AL, taking into account the prevalence, classification of the clinical forms and the results of laboratory analyses. The sample consisted of residents in endemic areas. The participants were submitted to a stool examination by the Kato-Katz technique and the diagnosis was based on the reading of two microscopic slides for each sample. The patients whose examinations were positive for schistosomiasis mansoni were submitted to a clinical examination and blood collection. Based on this examination, 8.11 percent of the study population were positive for schistosomiasis. The medium parasite load was 79.1 ± 174.3 eggs. The intestinal (90.57 percent) and hepatointestinal (9.43 percent) forms were found at statistically significant levels (p < 0.001). The results of the present study update information on schistosomiasis in the city of Rio Largo. These data, although referring only to three locations in that city, suggest a decrease either in the parasite load or in the severity of clinical forms.

Establishment of hemolytic anemia rat model and its laboratory evaluation / 重庆医科大学学报

Objective:Study on establishing HA animal model,which isoperated simply and have short model building period and high successful rate,to provide favorable test materials for HA-related scientific research.Methods:10 SD rats were randomly selected to set up the model by using APH intraperitoneal injection,meanwhile rats intraperitoneally infused with saline served as controls group.After 8 days of experimental treatment,we evaluated the animal model by using blood analysis,reticular count,myelogram and other laboratory experimental results.Results:On eighth day after experimental treat,the results of RBC、HGB、PLT、Ret% in Rats'peripheral blood are respectively(2.98?0.623)?1012/L、(100?14.2)g/L、(320?358.8)?109/L、90.7%?4.23%,the concentration of indirect bilirubin and free-haemoglobin in plasma are respectively(10.3?3.30)?mol/L、(1 248?282.1)mg/L.Compared to the control group,the difference of the results all have the statistical significance;bone marrow exhibited that karyocytes proliferate obviously,mainly the erythroid system.Conclusion:APH intraperitoneal injection can set up HA animal model shortly and conveniently,and the animal model has the clinical features.So the method of APH intraperitoneal injection is an ideal way to establish HA animal model