RESUMO
Objective To investigate the expression of hypoxia inducible factor 1α (HIF-1α) ,glucose trans-porter-1 (GLUT-1) and lactate dehydrogenase-5 (LDH-5) in gastric cancer tissues and their correlation with pathological features.Methods From June 2015 to June 2017 ,93 cases of gastric cancer resection were select-ed as the observation group ,and the cancer adjacent tissues were taken as the control group.Immunohisto-chemical staining was used to detect the expression of HIF-1α ,GLUT-1 and LDH-5.The gastric cancer tissues from 93 patients with gastric cancer who underwent gastrectomy in the hospital were taken as the observation group ,and the adjacent tissues were taken as the control group.The expressions of HIF-1α ,GLUT-1 and LDH-5 in two groups were determined by Immunohistochemical MaxVision Ⅲ method.The positive expres-sions of HIF-1α ,GLUT-1 and LDH-5 in two groups were compared ,and the correlation between HIF-1α , GLUT-1 and LDH-5 in gastric cancer tissues ,and the expressions of HIF-1α ,GLUT-1 and LDH-5 in different sex ,age ,clinical stage and differentiation degree were compared.Results The positive expression rates of HIF-1α ,GLUT-1 and LDH-5 in the observation group were higher than those in the control group ,and the differences were statistically significant (P＜ 0.05) ;HIF-1α ,GLUT-1 and LDH-5 were positively correlated with each other ;there were no significant differences of HIF-1α ,GLU T-1 and LDH-5 levels in different gender group and different age group (P＞0.05) ;the positive expression rates of HIF-1α ,GLU T-1 and LDH-5 at Ⅲ and Ⅳ stages were higher than those at Ⅰ and Ⅱ stages ,the positive expression rates of HIF-1α ,GLUT-1 and LDH-5 of low differentiation were higher than those of high and middle differentiation and the differences were statistically significant (P＜ 0.05).Conclusion The high expressions of HIF-1α ,GLUT-1 and LDH-5 were observed in gastric cancer tissue.HIF-1α ,GLUT-1 and LDH-5 were linearly and positively correlated with each other ,and had a significant correlation with the clinicopathological features.With the increase of clinical stage and the degree of differentiation ,the expression of HIF-1 a ,GLUT-1 and LDH-5 were higher.
RESUMO
Objective:To study the curative efficacy of decitabine combine with CAG regimen in the treatment of acute myeloid leukemia and its effects on the serum Interferon-γ (IFN-γ),alpha hydroxybutyrate dehydrogenase (HBDH) and lactate dehydrogenase (LDH) levels.Methods:70 cases of patients with acute myeloid leukemia who were treated from February 2013 to October 2016 in our hospital were selected as research objects.According to the random number table,the patients were divided into the observation group (n=35) and the control group (n=35).Both groups of patients were treated conventional treatment.The control group was treated with CAG scheme,intravenous injection of aclarubicin,20 mg each time,granulocyte colony stimulating factor,300 μg each time,subcutaneous intravenous cytarabine,10～15 mg/m2 each time,while the observation group was treated with intravenous drip of decitabine on the basis of control group,15 mg/m2 each time.Then the therapeutic effect,serum interferon-γ (IFN-γ),alpha hydroxybutyrate dehydrogenase (HBDH),lactate dehydrogenase (LDH) levels before and after treatment,incidence of adverse reactions were compared between two groups.Results:After treatment,the total effective of observation group was significantly higher than that of the control group [91.42％(32/35)vs68.57％(24/35)] (P ＜0.05);the serum IFN-γ,HBDH,LDH levels were significantly higher than those of the control group [(3.21± 1.01)pg/ml vs.(5.13 ± 1.90)pg/mL,(103.62± 26.39)U/L vs.(118.80± 28.60)U/L,(101.36± 27.32)U/L vs.(123.08 ± 30.59)U/L] (P ＜0.05);the incidence rate of adverse reactions was significantly lower than that of the control group [20.00％(7/35) vs.42.85％(15/35)(P ＜0.05)].Conclusion:Decitabine combined with CAG regimen was more effective for acute myeloid leukemia than CAG regimen alone,which might be related to reduce the serum levels of IFN-γ, HBDH and LDH.