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OBJECTIVE To evaluate the quality of total flavonoids of Lamiophlomis rotata from different producing areas. METHODS Total flavonoids of S1-S15 batches of L. rotata from different producing areas were extracted by percolation and purified by polyamide column. The content of total flavonoids was determined by UV spectrophotometry, and its purity was calculated. HPLC fingerprint chromatograms and control fingerprint of total flavonoids from 15 batches of L. rotata were established with Similarity Evaluation System of Chromatographic Fingerprint of TCM (2012 version). Their similarities were analyzed. Cluster analysis, principal component analysis and orthogonal partial least squares-discriminant analysis were used to evaluate the quality of total flavonoids from 15 batches of L. rotata, and the main components which affected the quality of total flavonoids were analyzed. RESULTS The purities of total flavonoids from 15 batches of L. rotata were 55.82%-94.12%, with an average value of 77.72%; a total of five common peaks were identified in the fingerprint, and No. 3 peak was luteolin; the similarities between the fingerprint of each batch of samples and the control fingerprint were 0.925-1.000. By cluster analysis, S1 and S3-S9 were clustered into the first class, which were samples from Qinghai Province and Tibet Autonomous Region. S14 and S15 were clustered into the second class, which were samples from Yunnan Province. S10-S13 were clustered into the third class, which were all samples from Sichuan Province. S2 was clustered into the fourth class. The principal component analysis showed that the qualities of samples from the first and fourth classes were better; peaks 2, 3 and 5 were identified as the main components that caused the differences among different batches of samples by orthogonal partial least squares-discriminant analysis. CONCLUSIONS The qualities of total flavonoids of L. rotata from Qinghai Province and Tibet Autonomous Region are better.
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Tibetan medicine Lamiophlomis rotata(Benth.)Kudo is a plant belonging to the genus L.rotata(Benth.)in Labiatae,which is a commonly used bulk Tibetan medicine.As a first-class endangered Tibetan medicine,its sustainable utilization of resources is representative,and it is particularly important to master its species characteristics and adaptation mechanism under high Plateau adversity.This paper sorts out the habitat characteristics,morphological characteristics,population ecological characteristics,reproductive characteristics and other aspects of L.rotata(Benth.),analyzes the growth characteristics and adverse ecological factors of L.rotata(Benth.)in the Qinghai Tibet Plateau grassland,and analyzes its adaptation mechanism in drought,cold and different degraded grass from two aspects of biological characteristics and physiological characteristics.The author believes that it is necessary to study its stress resistance mechanism from many aspects,such as gene and molecular biology,and explore the suitable planting mode of monoculture,so as to provide a theoretical basis for introduction and domestication,cultivation management,resource protection and sustainable utilization.
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Lamiophlomis Herba, one of the medicinal herbs commonly used by the Tibetan ethnic minority, has the effect of activating blood, stopping bleeding, dispelling wind, and relieving pain and is frequently used to treat trauma, traumatic bleeding, rheumatic arthralgia, and dampness and dampness-heat. By a review of ancient Tibetan medicine classics, materia medica classics, and modern literature, this paper systematically analyzed the name, nature, taste, original plant, medicinal part, harvest, processing, efficacy, and indications of Lamiophlomis Herba. The textual research showed that Lamiophlomis Herba was first recorded in the Somaratsa (《月王药诊》) in the middle of the eighth century. This medicinal herb was mainly recorded with a plain and warm nature and a sweet, bitter, and astringent taste. The herb was recorded as non-toxic in other books except the Tibetan Medicinal Plants in Gannan of Qinghai-Tibet Plateau (《青藏高原甘南藏药植物志》). In the books of the past dynasties, the aboveground part of Lomiophlomis rotatd was used as medicine. In addition, there were also records of using the whole herbs of Ajuga ovalifolia, A. ovalifolia var. calantha, and Oreosolen wattii as the medicine. In ancient times, the herb was mainly harvested before Frost's Descent in the 8th-9th months of the lunar calendar, while it was mainly harvested during the flowering-fruiting stage in autumn in modern times. Due to the decreased reserve of Lamiophlomis Herba, the medicinal part evolved from whole herb or fresh leaves to the aboveground part. According to the ancient and modern records, this herb mainly has the functions of nourishing bone and marrow, eliminating dampness and dampness-heat, stopping bleeding, and relieving pain. In ancient times, it was used for treating parasitic diseases, menostaxis, spermatorrhea, diarrhea, and nourishing the body. This paper aims to provide a basis for further development and study of Lamiophlomis Herba through the textual research.
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OBJECTIVE:To study the effects and active ingr edients of Tibetan medicine Lamiophlomis rotata against rheumatoid arthritis (RA). METHODS :Fifty-six SD rats were randomly divided into normal control group (0.5% sodium carboxymethylcellulose),model group (0.5% sodium carboxymethylcellulose ),methotrexate group (positive control group ,3 mg/kg),L. rotata water extract low-dose ,medium-dose and high-dose groups (0.5,1,2 g/kg,by crude drug ),L. rotata total flavonoid group (200 mg/kg,by flavonoid extat ),with 8 rats in each group. Except for normal control group ,other groups were given Freund ’s complete adjuvant (FCA)on the rat ’s right hind footpad to induce adjuvant-induced arthritis model. The next day after injection of FCA ,rats in all groups were given relevant medicine intragastrically ,once a day (once every 3 days for methotrexate group )for 30 days. At 15th and 30th day of administration ,the degree of paw swelling of left hind foot was measured,and the arthritis index ,spleen index were calculated. At the end of 30th day of administration ,the levels of TNF-α,IL-1β, IL-6 and IL- 10 in rat serum were determined by ELISA assay ,the thymus index and spleen index were calculated ,the pathological changes of the ankle joints were observed by HE staining. RESULTS :Compared with normal control group ,degree of paw swelling,arthritis index at 15th and 30th day of administration as well as the spleen index and the levels of TNF-α,IL-1β, msxmX0146) IL-6 in serum at 30th day of administration were significantly com increased in model group (P<0.01);while the thymus indexyunbinji- and IL- 10 level at 30th day of administration were ang@swu.edu.cn significantly decreased(P<0.01);synovial cell proliferation and infiltration of articular cavity were observed in ankle joint. Compared with model group ,degree of paw swelling ,arthritis index at 15th and 30th day of administration as well as the spleen index and the levels of TNF-α,IL-1β,IL-6 in serum at 30th day of administration were significantly decreased in each medicine group (P<0.01);while the thymus index and IL- 10 level at 30th day of administration were significantly increased (P<0.01);the pathological changes of arthritis were significantly improved. Compared with L. rotata water extract high-dose group ,there were no significant differences in degree of paw swelling at 15th day of administration as well as the arthritis index ,spleen index ,levels of inflammatory cytokines and pathological changes of ankle joint in L. rotata total flavonoid group (P>0.05). CONCLUSIONS :Tibetan medicine L. rotata shows well anti-RA activity ,and total flavonoids may be the active ingredients of its efficacy.
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AIM To study the chemical constituents from Lamiophlomis rotata (Benth.) Kudo.METHODS The 50% ethanol extract from L.rotata was isolated and purified by silica,ODS and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Ten compounds were isolated and identified as decaffeoylacteoside (1),markhamioside A (2),gentioside (3),6-O-acetylshanzhiside methyl ester (4),phloyoside Ⅰ (5),7,8-dehydropenstemoside (6),phlorigidoside C (7),β-daucosteol (8),caffeic acid (9),n-hexadecanoic acid (10).CONCLUSION Compounds 1-3 are isolated from this plant for the first time.
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Objective@#To investigate the correlation between the syndrome types of traditional Chinese medicine (TCM) and clinical symptoms of benign prostatic hyperplasia (BPH) with chronic prostatitis (BPH-CP).@*METHODS@#We selected 150 cases of BPH-CP in this study and divided them into 7 TCM syndrome types. Using univariate and multivariate logistic regression analyses, we studied the correlation of each TCM syndrome type with the age, disease course, prostate volume, postvoid residual urine volume (PVR), prostate-specific antigen (PSA) level, maximum urinary flow rate (Qmax), and International Prostate Symptoms Score (IPSS).@*RESULTS@#Kidney-yin deficiency was correlated positively with the prostate volume but negatively with Qmax and IPSS; kidney-yang deficiency positively with the age and prostate volume but negatively with IPSS; the damp heat syndrome positively with the PSA level but negatively with the disease course, prostate volume and Qmax; the spleen-qi deficiency syndrome positively with the prostate volume but negatively with the disease course; liver-qi stagnation positively with the disease course but negatively with the age, prostate volume and PVR; the syndrome of qi stagnation and blood stasis positively with the disease course and IPSS but negatively with PVR; the syndrome of lung-heat and qi blockage positively with the age, Qmax and IPSS but negatively with the disease course.@*CONCLUSIONS@#The TCM syndrome types of BPH-CP are closely correlated to their clinical symptoms. The analysis of the clinical objective indexes of BPH-CP can provide some reliable evidence for accurate identification of the TCM syndrome type of the disease.
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Humanos , Masculino , Fatores Etários , Doença Crônica , Progressão da Doença , Nefropatias , Diagnóstico , Hepatopatias , Diagnóstico , Medicina Tradicional Chinesa , Tamanho do Órgão , Antígeno Prostático Específico , Sangue , Hiperplasia Prostática , Classificação , Diagnóstico , Prostatite , Classificação , Diagnóstico , Qi , Análise de Regressão , Esplenopatias , Diagnóstico , Avaliação de Sintomas , Classificação , Métodos , Micção , Deficiência da Energia Yang , Diagnóstico , Deficiência da Energia Yin , DiagnósticoRESUMO
Objective@#To study the therapeutic effect of Lamiophlomis Rotata Capsule (LRC) in the treatment of type ⅢB prostatitis.@*METHODS@#We randomly divided 225 patients with type ⅢB prostatitis into an experimental group (n =125) and a control group (n =120), the former treated orally with LRC at 3 capsules tid while the latter with tamsulosin hydrochloride sustained-release capsules at 0.2 mg qd, both for 4 weeks. We compared the therapeutic effects between the two groups of patients based on the National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) obtained before, immediately after and at 4 weeks after medication.@*RESULTS@#A total of 120 patients completed the treatment in the experimental group, which showed remarkable decreases as compared with the baseline in the pain score (5.30 ± 1.23 vs 14.68 ± 1.51, P0.05). The experimental group achieved even markedly lower scores than the controls in the pain symptom (5.30 ± 1.23 vs 13.67 ± 1.49, P<0.05), QoL (6.46 ± 0.93 vs 7.47 ± 0.88, P<0.05) and total NIH-CPSI (17.50 ± 2.77 vs 25.77 ± 2.01, P<0.05) but a higher urination symptoms score than the latter after medication (7.16 ± 1.04 vs 5.68 ± 1.34, P<0.05). At 4 weeks after drug withdrawal, the experimental group also showed significantly lower scores of the pain symptom (7.23 ± 1.03), QoL (6.58 ± 0.87) and total NIH-CPSI (22.18 ± 2.03) than the baseline (all P<0.05) and those in the control group (14.14 ± 0.98, 8.12 ± 0.72 and 26.89 ± 1.67) (all P<0.05). Apart from dizziness in 2 of the patients, who gave up medication halfway, no other obvious adverse reactions were observed during the experiment.@*CONCLUSIONS@#Lamiophlomis Rotata Capsule deserves to be recommended for the treatment of type ⅢB prostatitis for its safety and effectiveness in reducing the pain and improving the life quality of the patients.
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Humanos , Masculino , Cápsulas , Doença Crônica , Preparações de Ação Retardada , Medicamentos de Ervas Chinesas , Usos Terapêuticos , Manejo da Dor , Prostatite , Tratamento Farmacológico , Patologia , Qualidade de Vida , Tansulosina , Usos Terapêuticos , Micção , Agentes Urológicos , Usos TerapêuticosRESUMO
Objective: The macroporous adsorptive resin chromatographic column combining with polyamide chromatographic column was used to separate the flavonoides, phenylethanoidglycosides, iridoid glycosides, and high polarity ingredients (such as alkaloid salts and sugars) in industrialization. Methods: Water extract from Lamiophlomis rotata (WELR) was separated and enriched by series and parallel method of polyamide and macroporous adsorptive resins and CCPP serial laminar analysis, and was ultrafiltrated by LM-125 ultrafiltration apparatus, then was dried under the reduced pressure by JYT-50 LN multipurpose kinesis back flow extract concentration subassembly. WELR, 70% ethanol solution eluate I (70% ethanol solution eluted polyamide column), 70% ethanol solution eluate II (70% ethanol solution eluting macroporous resin column), and ultrafiltration matter. The total flavonoids and total phenylethanoid glycosides were determined by aluminum nitrate-sodium nitrite UV spectrophotometry. The total iridoid glycosides were determined by the first derivative spectrophotometry. Shanzhiside methylester and 8-O-acetyl shanzhiside methylester were determined and the changes of total iridoid glycosides ingredients were investigated in the different elution requirement by HPLC. Results: Ethanol (70%) was used as dissolvent. Using the "introduction from superior surface" sample introduction mode and "introduction from superior surface" eluting mode in polyamide chromatographic column, the "introduction from inferior surface" sample introduction mode and "introduction from superior surface" eluting mode in the macroporous adsorptive resin chromatographic column, yields of 70% ethanol solution eluate I, 70% ethanol solution eluate II, and ultrafiltration matter were 2.43%, 20.80%, and 82.60%, respectively. Conclusion: This production technology may transform the experimental achievement to industrial production successfully.
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AIM : To set up a method of determinting the contents of four iridoid glycosides in different preparations of Lamiophlomis rotata ( Benth.) kudo.METHODS : The quantitative analysis was carded out on a column of Waters Symmetry by HPLC using a mobile phase of CH_3OH-H_2O under a flow rate of 1.0 mL/min.235 nm was selected as the detective wavelength.RESULTS: The linear ranges were 0.236-1.18 μg for sesamoside,0.440-2.20 μg for shanzhiside methyl ester,0.094-0.470 μg for 7,8-dehydropenste moside、0.750-3.75 μg for 8-O-ace-tylshanzhiside methyl ester.The recoveries of them were all between 96% and 104% ( RSD < 5% ).CONCLUSION: The method is accurate,stable,reliable,and can be used as a quantitative standard for determining the contents of iridoid glycosides in different Duyiwei preparations.
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OBJECTIVE: To establish an HPLC method for the determination of 8-O-acetyl-shanzhiside methylester in Tibet medicinal Lamiophlomis rotata.METHODS: HPLC analysis was performed on Symmetry C18(150 mm?4.6 mm,5 ?m) column with the mixture of acetonitrile-water(15∶85) served as the mobile phase.The detection wavelength was set at 234 nm.RESULTS:The linear range of 8-O-acetyl-shanzhiside methylester was 1.97~19.68 ?g?mL-1(r=0.999 6) and its average recovery was 99.53%(RSD=1.73%,n=9).CONCLUSION: The method is simple,accurate and specific,and it applies reference for quality evaluation and utilization of Tibet medicinal L.rotata.
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0.05).Conclusion: Lamiophlomis rotata capsule showed the similar curative effect and safety as Radix Notoginseng capsule did on dysmenorrhea.
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Objective To establish a method for the isolation and preparation of forsythoside B and acteoside reference substances from Lamiophlomis rotata.Methods Forsythoside B and acteoside in L.rotata were isolated and purified by macroporous resin,Sephadex column,and preparative HPLC.Results Analysis with HPLC showed the content of the prepared acteoside and forsythoside B reached to 98.93 and 99.91%,respectively.Conclusion This method is effective for the high purity of prepared acteoside and forsythoside B.It can be used as reference substances for the qualitative and quantitative analyses of Chinese herbal medicine.
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Objective To study the effect of Tibet medicine of Herba Lamiophlomis rotata on promoting hemostasis and blood coagulation and to explore its mechanism. Methods High dose(2 g/kg),middle dose(1 g/kg) and low dose (0.5 g/kg) of the aqueous extract from Herba Lamiophlomis rotata(HLRE)were given to mice by gastric gavage for 3 days,and then bleeding time (BT),clotting time(CT) and platelet count(PLC)were determined. Different doses of HLRE(3,1.5,0.75 g/kg)were administered by gastric gavage to rats for 14 days,and then blood samples were collected from common carotid artery for the determination of prothrombin time (PT),activated partial thromboplastin time (APTT),thrombin time(TT),fibrinogen(FIB),hepatic function and blood lipid indexes. Results Compared to the blank control group,the bleeding time and clotting time were obviously shortened in 2g/kg HLRE and 1g/kg HLRE groups and positive control group,but the difference of PLC was insignificant. PT and APTT values in all of the treatment groups did not differ from those in the blank control group. However, TT values were obviously shortened in 3 g/kg HLRE group,FIB and Albumen(Alb)values increased,the aminotransferase and blood lipid values tended to decrease. Conclusion The aqueous extract from Herba Lamiophlomis rotata has an effect on promoting hemostasis and blood coagulation ,and its mechanisms may be related to the increase of FIB and Alb contents and the shortening of TT value.
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AIM:To set up a method of determinting the contents of four iridoid glycosides in different preparations of Lamiophlomis rotata(Benth.)kudo.METHODS:The quantitative analysis was carried out on a column of Waters Symmetry by HPLC using a mobile phase of CH_3OH-H_2O under a flow rate of 1.0 mL/min.235 nm was selected as the detective wavelength.RESULTS:The linear ranges were 0.236-1.18 ?g for sesamoside,0.440-2.20 ?g for shanzhiside methyl ester、0.094-0.470 ?g for 7,8-dehydropenste moside、0.750-3.75 ?g for 8-O-acetylshanzhiside methyl ester.The recoveries of them were all between 96% and 104%(RSD
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OBJECTIVE:To study the effect of the hematostatic effective components of Herba Lamiophlomis rotata on rat’s blood conglomeration parameters.The toxicity of these components was simultaneously studied.METHODS:Herba Lamiophlomis rotata water extract (HLRE)(3g?kg-1),Total Flavonoids(P1,0.36g?kg-1),Total Iridoid Glycosides(P2,0.99g ?kg-1),Maximus Polarity Components(P3,1.65g?kg-1)and normal sodium NS were given orally in rats for 14 days.Blood samples were collected from common carotid artery;prothrombin time (PT),activated partial thromboplastin time (APTT),thrombin time (TT)and fibrinogen (FIB)were tested.Different oral doses of(P2 ,1.4,0.7,0.35 g?kg-1)and Yunnan white powder (BY,0.9g?kg-1)were given to rats and conglomeration parameters were determined too.The maximum tolerable dose (MTD)and LD50 of mice were determined after different doses of P2 were given with oral administration and intraperitoneal injection.RESULTS:Compared to NS control group,each composition of Lamiophlomis rotata Kudo can shorten the TT by 18.59%、—3.12%、24.11%and 9.92%respectively,and increase the content of FIB by 25.32%、8.67%、28.29%and 5.36%respectively.There was obvious change in HLRE and P2(P