Immunohistochemical study of amelogenin binding proteins in an amelogenin point mutation mouse / Estudio inmunohistoquímico de proteínas de unión de la amelogenina en un ratón con mutación puntual de amelogenina

Amelogenin is one of the enamel matrices secreted by ameloblasts. A mutation of the amelogenin gene can cause hereditary dental enamel defects known as amelogenesis imperfecta (AI). Since lysosome-associated membrane protein-1 (LAMP-1), -3 (LAMP-3), and 78kDa glucose-related protein (Grp78) were identified as binding proteins of amelogenin, several studies have suggested the involvement of these binding proteins with the cell kinetics of ameloblasts in normal or abnormal conditions. The purpose of this study is to investigate the distribution of these amelogenin binding proteins in the ameloblast cell differentiation of mice with a point mutation of the amelogenin gene (Amelx*). The incisors of Amelx* mice had a white opaque color and the tooth surface was observed to be rough under a scanning electron microscope. Among the sequential ameloblast cell differentiation in the Amelx* mice, the shape of ameloblasts at the transition stage was irregular in comparison to those in wild-type (WT) mice. Immunostaining of Grp78 revealed that the whole cytoplasm of the transition stage ameloblasts was immunopositive for Grp78 antibody, while only the distal part of cell was positive in the WT mice. Furthermore, in the Amelx* mice, the cytoplasm of the transition stage ameloblasts was immunopositive for LAMP-1 and LAMP-3. These results suggest that Amelx* may cause the abnormal distribution of amelogenin binding proteins in the cytoplasm of ameloblasts.

La amelogenina es una de las matrices de esmalte secretadas por los ameloblastos. Una mutación del gen de amelogenina puede causar defectos hereditarios del esmalte dental conocidos como amelogénesis imperfecta (AI). Dado que la proteína de membrana asociada a lisosoma-1 (LAMP-1), -3 (LAMP-3) y la proteína relacionada con la glucosa de 78 kDa (Grp78) se identificaron como proteína de unión a amelogenina, varios estudios han sugerido la participación de estas proteínas con la cinética celular de los ameloblastos en condiciones normales o anormales. El objetivo del estudio fue investigar la distribución de LAMP-1, LAM-3 y Grp78 durante la diferenciación celular de ameloblastos de ratones con una mutación puntual del gen de amelogenina (Amelx*). Los incisivos de los ratones Amelx* presentaron un color blanco opaco y se observó en microscopio electrónico de barrido que la superficie del diente era áspera. La diferenciación celular secuencial y la forma de los ameloblastos en la etapa de transición en los ratones Amelx* fue irregular en comparación con los ratones silvestres (RS). La inmunotinción de Grp78 reveló que todo el citoplasma de los ameloblastos en etapa de transición fue inmunopositivo para el anticuerpo Grp78, mientras que solo la parte distal de la célula fue positiva en los ratones RS. Además, en ratones Amelx*, el citoplasma de los ameloblastos en etapa de transición fue inmunopositivo para LAMP-1 y LAMP-3. Estos resultados sugieren que Amelx* puede causar distribución anormal de proteínas de unión a amelogenina en el citoplasma de los ameloblastos.

Evaluation of efficiency of traps baited with frequency trembler grid lamps and trap plants for control Heortia vitessoides infectwed in Aquilaria sinensis / 中国中药杂志

Heortia vitessoides has been a serious defoliating pest of Aquilaria sinensis forests in recent years.The adults displayed strong tropism to the frequency trembler grid lamps and the nectar source plants.The favorite nectar source plants of H.vitessoides adults as the trap plants and the frequency trembler grid lamps in the integrated management of H.vitessoides were studied in the adult eclosion period through both the laboratory and field.The results showed that Kuhnia rosmarnifolia and Santalum album plants showed strong attraction to the H.vitessoides adults, with significant differences among the different nectar source plants.K.rosmarnifolia and S.album as trap plants with board type of planting area to total planting area of 5%-10%, and the frequency trembler grid lamps trapped significantly more adults of H.vitessoides. These results suggested that the frequency trembler grid lamps and trap plants could play an important role in the integrated management of the pest H.vitessoides of A.sinensis.

Occupational hazard risk assessment with Singapore exposure semi -quantitative evaluation methods in energy -saving lamp industry / 预防医学

Objective To explore and establish an appropriate occupational risk assessment model for energy -saving lamp industry in China,and further to provide reference for risk management.Methods Based on actual conditions on site of 3 different scale energy -saving lamp manufacture factories,Singapore exposure semi -quantitative evaluation methods was used to analyze and evaluate the harm of the work sites contacted with chemical factors,according to which can provide a proposal for risk management.Results The primary occupational chemical hazards in these 3 factories include carbon monoxide,carbon dioxide,aluminium oxide dust,butyl acetate,mercury metal(vapor)and cement dust(free SiO2 <10%).Mercury -injecting of the big scale one has medium risk besides all work sites have low risks or below.Conclusion This methods has confirmed the actual risk levels of the work sites,using the test data in work place,but has a shortage while the sites contacted with physical agents such as noise and heat.

Análise da eficiência energética, ambiental e econômica entre lâmpadas de LED e convencionais / Analysis of energy efficiency, environmental and economical between LED and conventional lamps

RESUMO Os Diodos Emissores de Luz, mais conhecidos como LED's, são componentes eletrônicos semicondutores que conseguem transformar a energia elétrica em luz, diferente das lâmpadas convencionais. O LED é um componente bipolar, que quando passa corrente elétrica, a luz é gerada. As lâmpadas modernas utilizam LED em sua formação, as quais liberam menos calor e utilizam menos energia, sendo assim mais econômicas que as incandescentes e as fluorescentes. Além de apresentarem maior durabilidade que as demais, as lâmpadas LED não promovem o aquecimento dos ambientes internos e seu descarte tem reduzido impacto ambiental. A associação de vantagens ambientais e econômicas torna esta tecnologia atrativa ao consumidor, embora o custo para sua aquisição seja superior aos demais modelos de lâmpadas disponíveis no mercado. Neste estudo foram realizados testes de verificação do fluxo luminoso e eficiência energética, além de análise ambiental das lâmpadas domésticas. Os resultados obtidos comprovaram o potencial da tecnologia LED em relação às demais lâmpadas analisadas, especialmente quanto à questão econômica e ambiental conforme estudos prévios analisados.

ABSTRACT Light Emitter Diode, more commonly known as LED´s, are semiconductor electronic components that can transform electrical energy into light, unlike conventional lamps. LED has a bipolar component, when polarized, allows the passage of electric current creating light. Modern lamps use LED in their formation, which release less heat, use less energy, and are more economic than incandescent and fluorescent. Besides present more durability than the others, LED lamps do not heat up the environment and their disposals do not attack the environment. The association of environmental and economic advantages makes this technology attractive to consumer, although the cost of acquisition is higher than the other lamps models available in market. The verification of luminous flux and energy efficiency tests were realized in this study, beyond an environmental analysis. The obtained results proved the potential of LED technology due to other analyzed lamps, especially economic and environmental issue as previous studies analyzed.

The lipid-associated membrane proteins derived from Mycoplasma pneumoniae strains induced the expression of HO-1 in THP-1 cells / 中华微生物学和免疫学杂志

Objective To investigate the effects of lipid-associated membrane proteins ( LAMPs) derived from Mycoplasma pneumoniae ( M.pneumoniae) strains on the expression of heme oxygenase 1 ( HO-1) in a human monocyte cell line (THP-1).Methods THP-1 cells were in vitro cultured with different concentrations of LAMPs for different times.The cytotoxicity of LAMPs to THP-1 cells was analyzed by using lactate dehydrogenase ( LDH) releasing test.The expression of HO-1 at protein and mRNA levels were de-tected by Western blot and real-time RT-PCR, respectively.The enzymatic activity of HO-1 protein was ex-amined by colorimetric assay.THP-1 cells stimulated with PBS and LPS were set up as the negative and pos-itive controls, respectively.Results A significantly enhanced LDH releasing rate was observed in THP-1 cells treated with 10 μg/ml of LAMPs.The expression of HO-1 at protein and mRNA levels in THP-1 cells were induced by LAMPs in a dose-dependent and time-dependent manner.The highest level of HO-1 protein was detected in THP-1 cells treated with 5.0 μg/ml of LAMPs.The transcriptional levels of HO-1 induced by LAMPs were significantly elevated at 3 h, peaked at 9 h and were decreased at 12 h.The expression of HO-1 protein in THP-1 cells was enhanced after 8 h of treatment with LAMPs and a significant decrease was observed at 20 h after reaching peaks at 12 h and 16 h.The activity of HO-1 protein was significantly en-hanced along with the increased expression of HO-1 protein.Conclusion The LAMPs derived from M.pneumoniae strains induced the expression of HO-1 at mRNA and protein levels.Moreover, the enzyme activity of HO-1 protein was enhanced in LAMPs treated THP-1 cells.

Immunohistochemical study of amelogenin and lysosome-associate membrane proteins (LAMPs) in cartilage / Estudio inmunohistoquímico de la amelogenina y proteínas de membrana asociadas a lisosomas (LAMPs) en el cartílago

Amelogenin is one of the enamel matrix proteins secreted by ameloblasts during enamel formation in tooth development. Recent studies showed that the amelogenin is expressed in chondrocyte. Lysosome-associated membrane proteins (LAMPs) have been identified as binding partner proteins to amelogenin and it has been suggested they act as signaling receptors of amelogenin. The purpose of this study is to clarify the localization of amelogenin and LAMPs in growth plate cartilage and cartilaginous nodules in micromass culture. Mouse knee joints including tibia growth plate at 4 weeks old and micromass cultures of limb bud mesenchymal cells after 2 weeks were fixed in paraformaldehyde, routinely processed, sections were cut and immunostained with amelogenin, collagen type II and type X, LAMP-1 and -3. The positive immunoreaction of amelogenin was observed both in proliferation and hypertrophic zone cartilage of growth plate after enzymatic pretreatment in immunostaining. Furthermore, cartilaginous nodules in micromass culture were immunopositive to amelogenin. The chondrocytes in the proliferation zone of the growth plate were immunopositive to LAMP-1 but weakly stained in the chondrocytes of hypertrophic zone. These observations indicate that amelogenin may be present in cartilage matrix produced in vivo and in vitro and amelogenin may involve cartilage formation through the LAMP-1 signaling pathway.

La amelogenina es una de las proteínas de la matriz del esmalte secretadas por ameloblastos durante la formación del esmalte en el desarrollo dentario. Estudios recientes demuestran que la amelogenina se expresa en los condrocitos. Las proteínas de membrana asociadas a lisosomas (LAMPs) se han identificado como proteínas de unión asociadas a la amelogenina; se ha sugerido que actúan como receptores de señalización de la amelogenina. El propósito de este estudio fue aclarar la localización de la amelogenina y las LAMPs en el cartílago de crecimiento y nódulos cartilaginosos en cultivos de micromasa. Articulaciones de la rodilla del ratón, que incluían la placa de crecimiento tibial de 4 semanas de edad y cultivos de micromasa de células mesenquimales del brote del miembro después de 2 semanas se fijaron en paraformaldehído y procesaron rutinariamente. Los cortes fueron sometidos a inmunotinción con amelogenina, colágeno tipo II y X, LAMP-1 y LAMP-3 . Se observó inmunorreacción positiva de amelogenina tanto en la zona proliferación e hipertrófica del cartílago de crecimiento después del pretratamiento enzimático. Además, los nódulos cartilaginosos en el cultivo de micromasa eran inmunopositivos para la amelogenina. Los condrocitos en la zona de proliferación de la placa de crecimiento fueron immunopositivos a LAMP-1, mientras que los condrocitos de la zona hipertrófica se tiñeron débilmente. Estas observaciones indican que la amelogenina puede estar presente en la matriz del cartílago producida tanto in vivo e in vitro, además la amelogenina puede estar implicada en la formación de cartílago mediante la vía de señalización de LAMP-1.

Estudo sobre reciclagem de lâmpadas fluorescentes / Study about fluorescent lamp recycling

Lâmpadas fluorescentes (LF) contêm mercúrio em sua composição, um metal pesado que pode causar danos à saúde e ao meio ambiente. As LF são amplamente consumidas no Brasil, porém o descarte correto de LF usadas não é amplamente divulgado e os índices de reciclagem são baixos. O objetivo deste estudo foi demonstrar a relevância do descarte adequado, avaliar as tecnologias de reciclagem e a destinação dos materiais, por meio de estudo comparativo entre Brasil e Alemanha. Através de visitas a recicladoras e análise das tecnologias adotadas nos dois países, foi verificado que no Brasil o vidro é destinado à indústria de cerâmica, enquanto que na Alemanha é destinado à fabricação de tubos para LF. Além disso, foi observado que o mercúrio apresenta diversas possibilidades de uso. Um ponto a ser explorado nos dois países, e de grande potencial para reciclagem, é a recuperação de terras raras do pó fosfórico presente nas LF. Tanto Brasil como Alemanha possuem oportunidades de melhoria na logística reversa de LF, por meio da qual se torna possível elevar os índices de reciclagem e reduzir os impactos ambientais causados pelo descarte inadequado de LF usadas.

Fluorescent lamps (FL) contain mercury in their composition, a heavy metal that can be a health hazard and damage the environment. The FL are widely consumed in Brazil, although the right disposal of FL is not well known and the recycling rate is low. This study objective is to demonstrate the importance of proper disposal and to evaluate recycling technologies and material destination through a comparative study between Brazil and Germany. In visits to recycling companies in both countries and analyzing the used technologies, it was verified that in Brazil the glass is sent to ceramics industry, while in Germany it is mainly sent for the manufacture of FL tubes. Also, the mercury has several applications. A point to be explored in both countries, with great recycling potential, is the recovery of the rare earth from phosphoric powder from FL. Both Brazil and Germany have improvement opportunities in the reverse logistics of FL, through which it becomes possible to increase recycling rates and reduce the environmental impact caused by the inappropriate disposal of used FL.

Mercury Concentration in air and in Urine of Workers in Fluorescent Lamp Manufacturing Factories in Korea / 대한산업의학회지

This research was conducted to investigate the correlations between urinary mercury concentration and each independent variable related with urinary mercury levels. the urinary mercury concentrations of 543 workers exposed to metal mercury vapor in a total of 11 fluorescent lamp manufacturing factories and at the same time mercury concentrations in air were measured from June 1989 to December 1989. And annually mercury consumption per workers, mercury consumption per lamp, numbers of breakage lamps, frequency of mercury infusion, numbers of droppers, and numbers of vacuum exhaustion pumps were also investigated. The results were as follows: 1. The geometric mean of airborne mercury concentration in a total of 11 factories was 47.9 microgram/m3 (5.8~352.2 microgram/m3), six factories(54.5%) of them were exceed the threshold limit value(50.0 microgram/m3). 2. The geometric mean of urinary mercury concentration among 543 workers was 84.3 microgram/l (1.13~533.9 microgram/l), the distribution of workers by urinary mercury concentration showed that 26 workers(4.8%) were above the mercury posioning level(300 microgram/l). 3. The correlation coefficient between urinary mercury concentration and monthly numbers of breakage lamps was the highest(0.74) and next was mercury consumption per lamp (0.67), annually mercury consumption per worker(0.63) in order.