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Chinese Journal of Natural Medicines (English Ed.) ; (6): 30-37, 2014.
Artigo em Inglês | WPRIM | ID: wpr-812312

RESUMO

AIM@#To evaluate the chemical profiles and cytotoxic effects among the total saponin fraction (TSF), 25% ethanol fraction (25EF), 50% ethanol fraction (50EF), and 85% ethanol fraction (85EF) prepared by macroporous resin from the leaves of Panax notoginseng.@*METHOD@#The simultaneous determination of thirteen main saponins, as well as the chemical profiles of saponin fractions of different polarity, was made by HPLC-DAD and LC-ESI-MS(n) analysis. The cytotoxic effects were determined against KP4 cells (human pancreatic cancer), NCI-H727 cells (human lung cancer), HepG2 cells (human hepatocellular cancer), and SGC-7901 cells (human gastric adenocarcinoma).@*RESULTS@#Chemical analysis indicated that 85EF possessed the most abundant cytotoxic protopanaxadiol saponins, including the marker saponins F2, 20(R)-Rg3, 20(S)-Rg3, and Rh2. The MTT assay showed that 85EF also had the strongest cytotoxic effects among the four fractions. 25EF showed no anti-proliferative effects, while 50EF and TSF exhibited weak anti-proliferative activity.@*CONCLUSION@#From the aspect of comprehensive utilization of resources, 85EF, enriched with low polarity PPD group saponins, is a new alternative source of anticancer saponins, and a promising botanical preparation for further anticancer studies.


Assuntos
Humanos , Antineoplásicos Fitogênicos , Química , Farmacologia , Linhagem Celular Tumoral , Proliferação de Células , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas , Química , Farmacologia , Espectrometria de Massas , Panax notoginseng , Química , Folhas de Planta , Química , Saponinas , Química , Farmacologia
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