Susceptibility of common bean and soybean to water stress evaluated at the sitis phenotyping platform / Suscetibilidade do feijão-comum e da soja à deficiência hídrica avaliada na plataforma de fenotipagem sitis

The great challenge of breeding programs focused on tolerance to water stress is the precise, in large scale, and automated phenotyping. Therefore, the objective of this study was to assess whether the controlled conditions of SITIS Automated Phenotyping Platform and the protocol used are suitable for discriminating cultivars of common bean and soybean for tolerance to this stress. Two experiments were carried out in randomized block design, in a split plot scheme, with four replications. The main plots had five water regimes, applied after flowering: daily replacement of 100 (control), 80, 60, 40, and 20% of water evapotranspired in control. The subplots consisted of two common bean cultivars (BRS Pontal and BRS Pérola) in the 1st experiment, and two soybean cultivars (MG/BR 46 Conquista and BR- 16) in the 2nd one. In each species, the first cultivar is more tolerant to water stress, and the second one is more susceptible. It is possible to use the SITIS Platform and the proposed protocol to evaluate common bean and soybean cultivars for tolerance to water stress. The common bean cultivars evaluated were more sensitive to this stress than soybeans. The best water status of cultivars BRS Pontal and MG/BR 46 Conquista under water stress confirmed their greater tolerance.

O grande desafio dos programas de melhoramento com foco na tolerância à deficiência hídrica é a fenotipagem precisa, em larga escala e automatizada. Assim, objetivou-se avaliar se as condições controladas da Plataforma Automatizada de Fenotipagem SITIS e o protocolo utilizado são adequados para discriminar cultivares de feijão e soja quanto à tolerância a esse estresse. Foram conduzidos dois experimentos em blocos ao acaso, em parcelas subdivididas, com quatro repetições. Nas parcelas foram conduzidos cinco regimes hídricos, aplicados após a floração: reposição diária de 100 (controle), 80, 60, 40 e 20% da água evapotranspirada no controle. As subparcelas consistiram de duas cultivares de feijão (BRS Pontal e BRS Pérola), no 1º experimento, e duas de soja (MG/BR 46 Conquista e BR-16), no 2º. Em cada espécie, a primeira cultivar é mais tolerante à deficiência hídrica e a segunda é mais suscetível. É possível usar a Plataforma SITIS e o protocolo proposto para avaliar cultivares de feijão e soja quanto à tolerância à deficiência hídrica. As cultivares de feijão foram mais sensíveis a esse estresse do que as de soja. O melhor status hídrico das cultivares BRS Pontal e MG/BR 46 Conquista sob deficiência hídrica confirmou sua maior tolerância a esse estresse.

Pérdida de pubescencia foliar y sus efectos fisiológicos en Espeletia paipana(Asterales, Asteraceae), en el departamento de Boyacá-Colombia / Loss of leaf pubescence and its physiological effects on Espeletia paipana (Asterales, Asteraceae), in the Boyacá-Colombia department

Espeletia paipanaes una roseta caulescente gigante en peligro de extinción, endémica del departamento de Boyacá. Con el fin de establecer si una enfermedad en la planta, atribuida a la acción patogénica de microorganismos endófitos y caracterizada por la pérdida de pubescencia foliar (PPF), puede ser causante de la acelerada mortandad poblacional, se evaluó el desempeño fisiológico de la especie con y sin PPF. La incidencia (% de hojas afectadas por cada uno de los 27 individuos de la población actual) y severidad (% de área foliar afectada en 135 hojas) de la PPF fueron monitoreadas durante un periodo de nueve meses, en tres zonas topográficas a diferentes altitudes. Parámetros fisiológicos como conductancia estomática (Gs) y temperatura foliar (Tfol) e índice de contenido de clorofila (ICC) fueron medidos en hojas sanas y enfermas durante cuatro días consecutivos, tanto en la época seca como en la época húmeda. El estudio fue complementado con aislamientos y pruebas de patogenici-dad para identificar el agente causal de la PPF. En general, aunque la incidencia de la enfermedad en E. paipanafue constante a lo largo del tiempo, la severidad avanzó superando el 60 % del área foliar. El aumento de la severidad en el haz fue atribuido al efecto foto-oxidativo de las altas radiaciones entre las 11:00 y14:00 h. La reducción del área foliar funcional a causa de la PPF, llevó a una baja Gs con serias implicaciones en la fijación de carbono, y por tanto limitando el crecimiento y la renovación de biomasa. El efecto estacional en la Tfol varió de acuerdo con la zona topográfica, mientras que el ICC no presentó un patrón definido con relación a la PPF; sus valores bajos podrían estar relacionados con la producción de otros pigmentos. Finalmente, aunque no es posible asegurar que Botrytissp. es el causante de la pérdida de pubescencia foliar, se postula como el agente causal más probable debido a su alta representatividad en los aislamientos y su potencial de infección durante las pruebas de patogenicidad. En general, la reducción de biomasa foliar sana y disminución del rendimiento fisiológico sugieren que la PPF afecta negativamente la sobrevivencia de E. paipana,lo que significa que el uso de biocontroladores podría ser una estrategia para el rescate poblacional.

Espeletia paipanais an endangered giant caulescent rosette endemic to Boyacá department. In order to establish whether a plant disease, characterized by the loss of leaf pubescence (PPF) and attributed to the pathogenic action of endophytic microorganisms, is the cause of the increasing mortality of population, the physiological performance of the species was evaluated with and without PPF. The incidence (% leaves affected in each of the 27 individuals in the current population) and severity (% leaf area affected on 135 leaves) of the PPF were monitored over a period of nine months, in three topographic zones of different heights. During four consecutive days in both dry and wet season, physiological parameters as chlorophyll content index (ICC), stomatal conductance (Gs) and leaf temperature (Tfol) were measured in healthy and affected leaves. The study was complemented with isolations and pathogenicity tests to identify the causal agent of the PPF. Overall, although the disease incidence in E. paipanawas constant over time, the severity progressed surpassing 60 % of the leaf area. The increasing of severity in the upper side of leaves was attributed to the photo-oxidative effect of high radiation between 11:00 h and 14:00 h of the day. The reduction of functional leaf area because of the PPF, led to low Gs with serious implications for carbon fixation and thus limiting growth and biomass renewal. The effect of season in Tfol varied according to the topographic zone, while the ICC did not present a defined pattern with respect to the PPF; its low values could be associated with the production of other pigments. Finally, although it is not possible to ensure that Botrytissp. is the causative of the loss of leaf pubescence, it is postulated as the most probably causal agent due to its high representativeness in the isolates and its infectious potential during the pathogenicity tests. In general, the reduction of healthy leaf biomass and decrease of physiological performance suggest that PPF affect negatively the survival of E. paipana,which means that the use of biological controllers could be a strategy to mitigate its effect on the population.

Indicadores morfo-fisiológicos do estresse hídrico para a cultura da cana-de-açúcar em função de lâminas de irrigação / Morpho-physiological indicators of water stress on sugarcane as a function of irrigation depths

O presente trabalho foi desenvolvido, objetivando-se encontrar as relações entre lâmina de água (chuva e irrigação) e indicadores morfológico (índice de área foliar) e fisiológicos do estresse hídrico (temperatura foliar, potencial hídrico foliar e índice de clorofila nas folhas) na cana-de-açúcar submetida a diferentes lâminas de irrigação. O experimento foi instalado em outubro de 2010 em Jaíba/MG, em uma área irrigada por pivô central, com cana-de-açúcar no quarto ciclo. Para a aplicação dos tratamentos, trocaram-se os bocais dos emissores, em cada vão entre torres, de modo que fossem aplicadas as lâminas referentes a 25, 50, 75, 100, 125 e 150% da evapotranspiração da cultura, com o manejo da irrigação realizado para atender a demanda de 100%. Foram medidos o índice de área foliar, o potencial hídrico foliar, a temperatura foliar e o índice de clorofila nas folhas. Os valores obtidos foram submetidos a análises de variância e, posteriormente, elaboradas regressões. Diante dos resultados, foi possível concluir que houve tendência de aumento do índice de área foliar (IAF) da cana-de-açúcar à medida que se aumentou a lâmina de irrigação no ciclo da cultura; o aumento do IAF foi mais acentuado nos tratamentos que receberam mais água; o potencial hídrico foliar foi menor para menores lâminas de irrigação, com destaque para o tratamento que recebeu menos água (25%); a temperatura foliar foi próxima à temperatura do ar nos tratamentos que receberam as maiores lâminas (100 a 150%), com tendência de aumento da diferença entre a temperatura das folhas, à medida que se reduziu o suprimento hídrico; houve tendência de aumento do índice de clorofila (ICF) à medida que se aumentou as lâminas, com leve queda nos tratamentos que receberam lâminas maiores que 100% da ETc.

The present study was developed to evaluate relationships between the water depths (rain and irrigation) and morphological (leaf area index) and physiological indicators (leaf temperature, leaf water potential and chlorophyll content in leaves) of water stress on sugarcane subject to different irrigation levels. The experiment was installed in October 2010 in Jaíba/MG, Brazil, in an area irrigated by a center pivot system with sugarcane in the fourth cycle. For the treatments, emitter nozzles were exchanged in each vain between pivots so that the depths applied related to 25, 50, 75, 100, 125 and 150% of evapotranspiration, with the irrigation management performed to meet 100% of the demand. The factors of leaf area index, leaf water potential, leaf temperature and leaf chlorophyll index were measured. The values obtained were subjected to analysis of variance and subsequently developed regressions. From the results, it was concluded that there was a trend of increased leaf area index (LAI) for sugarcane as increasing the water depth in the crop cycle; the increase in LAI was more pronounced in treatments that received more water; leaf water potential was lesser for smaller irrigation depths, with emphasis on the treatment that received less water (25%); leaf temperature was close to the air temperature in the treatment receiving the highest depths (100 and 150%) with a trend for an increased difference between the temperature of the leaves as reducing the water supply; the chlorophyll index (ICF) tended to increase as increasing the depths, with a slight drop in treatments receiving depths greater than 100% of the ETc.